关键词: 雌激素受体alpha, 慢病毒载体, 293T细胞

Abstract: Objective To construct a lentiviral vector for over-expression of human estrogen receptor alpha (ERα) genefor further estimating the effects of ERα gene on tooth-derived stem cell research.Methods pEGFP-ERα was used as a template and ERα gene was amplified by RT-PCR.The right sequenced ERα gene was reorganized into the lentiviral vector PMSCV-GFP for construction of PMSCV-ERα.293T cells were transfected with PMSCV-ERα and the expression level of ERα protein was detected by Western Blot.Results ERα gene was amplified and PMSCV-ERα was constructed.Western Blot results showed that the expression level of ERα protein was much higher in PMSCV-ERα group than the control group (PMSCV-GFP).Conclusions A lentiviral vector for over-expression of human ERα was successfully constructed in our research.

Key words: Estrogen receptor alpha (ERα), Lentiviral vector, 293T cells