他米巴罗汀对牙龈卟啉单胞菌抗原刺激RAW264.7细胞的影响

›› 2015, Vol. 35 ›› Issue (5): 349-353.

他米巴罗汀对牙龈卟啉单胞菌抗原刺激RAW264.7细胞的影响

靳赢¹,王智明²,林晓萍¹

1. 中国医科大学附属盛京医院
2. 中国医科大学

收稿日期:2014-11-28 修回日期:2015-01-04 出版日期:2015-05-28 发布日期:2015-05-13
通讯作者: 林晓萍 E-mail:xiaoping_ba@126.com
基金资助:
高等学校博士学科点专项科研基金;辽宁省科学技术计划项目;沈阳市科技计划项目

Effect of Tamibarotene on P.gingivalis antigen-stimulated RAW264.7 cells

Received:2014-11-28 Revised:2015-01-04 Online:2015-05-28 Published:2015-05-13

摘要/Abstract

摘要： 目的探讨他米巴罗汀（Tamibarotene，Am80）对经牙龈卟啉单胞菌（Porphyromonas gingivalis，P.gingivalis）抗原刺激后的小鼠单核巨噬细胞RAW264.7的影响。方法采用P.gingivalis抗原刺激RAW264.7细胞，分别用不同浓度的Am80进行干预;相差显微镜下观察各组细胞生长情况；MTS法检测不同浓度Am80对抗原刺激RAW264.7细胞的增殖情况；酶联免疫吸附法（ELISA）检测细胞培养上清中抗炎因子IL-10的含量。结果 10 nmol/L Am80能够显著抑制P.gingivalis抗原刺激RAW264.7细胞的增殖分化（P<0.001）；Am80（10 nmol/L）致抗原刺激的细胞IL-10的分泌量显著增加（P<0.05）；倒置相差显微镜下观察显示Am80抑制抗原刺激细胞的增殖分化。结论 Am80（10 nmol/L）时可显著促进P.gingivalis抗原诱导小鼠巨噬细胞分泌抗炎因子IL-10，并可能由此抑制P.gingivalis抗原诱导的炎症反应。

关键词: 他米巴罗汀, 巨噬细胞, Interleukin-10, 牙龈卟啉单胞菌

Abstract: Objective To investigate the effect of Am80 on murine macrophage RAW264.7 after P.gingivalis antigen stimulation.Methods RAW264.7 cells were stimulated by P.gingivalis antigen and treated with different concentrations of Am80.Cell growth was observed in each group under a phase contrast microscope; the proliferation of antigen-stimulated RAW264.7 cells with different concentrations of Am80 were detected by MTS assay; enzyme-linked immunosorbent assay(ELISA) was used to detect anti-inflammatory factor levels of IL-10 in cell culture supernatant.Results 10 nmol/L Am80 significantly inhibited the proliferation and differentiation of P.gingivalis antigen-stimulated RAW264.7 cells（P<0.001）while Am80 (10 nmol/L) significantly increased the secretion of IL-10 by antigen-stimulated cells (P<0.05); inverted phase contrast microscope showed that Am80 inhibited antigen-stimulated proliferation and differentiation of RAW264.7 cells.Conclusions Am80 (10 nmol/L) can significantly promote P.gingivalis antigen-induced murine macrophages secretion of anti-inflammatory cytokine IL-10,and it may inhibit P.gingivalis antigen-induced inflammation.

Key words: Tamibarotene , macrophage , Interleukin-10, Porphyromonas gingivalis

中图分类号:

R780.2

靳赢王智明林晓萍. 他米巴罗汀对牙龈卟啉单胞菌抗原刺激RAW264.7细胞的影响[J]. 口腔医学, 2015, 35(5): 349-353.

参考文献

[1]Wang T,Nagai H,Bouda K,et al.Effect of selective IL-6 inhibitor Am-80 on experimental autoimmune encephalomyelitis in DA rats[J].Acta Pharmacol Sin,2000,21(11):967-976
[2]Keino H,Watanabe T,Sato Y,et al.Oral administration of retinoic acid receptor-alpha/beta-specific ligand Am80 suppresses experimental autoimmune uveoretinitis[J].Invest Ophthalmol Vis Sci,2011,52(3):1548-1556
[3]王琳源,关宁,林晓萍,等.全反式维甲酸对牙周炎小鼠辅助性T17细胞和调节性T细胞及细胞相关因子表达的影响[J].中国医科大学学报,2014,43(7):606-611
[4]Miwako I,Kagechika H.Tamibarotene[J].Drugs Today Barc,2007,43(8):563-568
[5]Takeda N,Manabe I,Shindo T,et al.Synthetic retinoid Am80 reduces scavenger receptor expression and atherosclerosis in mice by inhibiting IL-6[J].Arterioscler Thromb Vasc Biol,2006,26(5):1177-1183
[6]Miyabe Y,Miyabe C,Nanki T.Could retinoids be a potential treatment for rheumatic diseases[J].,2014,:-
[7]Jin Y,Wang L,Liu D,et al.Tamibarotene modulates the local immune response in experimental periodontitis[J].Int Immunopharmacol,2014,23(2):537-545
[8]Battaglino R,Kim D,Fu J,et al.c-myc is required for osteoclast differentiation[J].J Bone Miner Res,2002,17(5):763-773
[9]Chen D,Nie M,Fan M W,et al.Anti-inflammatory activity of curcumin in macrophages stimulated by lipopolysaccharides from Porphyromonas gingivalis[J].Pharmacology,2008,82(4):264-269
[10]叶洁,邓辉,徐春燕,等.脂多糖刺激单核细胞上清对成骨细胞Runx2_Cbf_1表达影响[J].口腔医学,2011,31(3):140-143
[11]Liu Y C,Zou X B,Chai Y F,et al.Macrophage polarization in inflammatory diseases[J].Int J Biol Sci,2014,10(5):520-529
[12]Sima C,Glogauer M.Macrophage subsets and osteoimmunology: tuning of the immunological recognition and effector systems that maintain alveolar bone[J].Periodontol 200,2013,63(1):80-101
[13]Kayal R A.The role of osteoimmunology in periodontal disease[J].Biomed Res Int,2013,2013:639368-
[14]Banchereau J,Pascual V,O'Garra A.From IL-2 to IL-37: the expanding spectrum of anti-inflammatory cytokines[J].Nat Immunol,2012,13(10):925-931
[15]Souza P P,Lerner U H.The role of cytokines in inflammatory bone loss[J].Immunol Invest,2013,42(7):555-622
[16]Sasaki H,Okamatsu Y,Kawai T,et al.The interleukin-10 knockout mouse is highly susceptible to Porphyromonas gingivalis-induced alveolar bone loss[J].J Periodontal Res,2004,39(6):432-441
[17]Wang L,Wang J,Jin Y,et al.Oral administration of all-trans retinoic Acid suppresses experimental periodontitis by modulating the th17/treg imbalance[J].J Periodontol,2014,85(5):740-750
[18]Mohamed S G,Sugiyama E,Shinoda K,et al.Interleukin-10 inhibits RANKL-mediated expression of NFATc1 in part via suppression of c-Fos and c-Jun in RAW2647 cells and mouse bone marrow cells[J].Bone,2007,41(4):592-602