白细胞介素-17基因多态性与侵袭性牙周炎的相关性研究

›› 2015, Vol. 35 ›› Issue (6): 417-420.

• 基础研究 • 下一篇

白细胞介素-17基因多态性与侵袭性牙周炎的相关性研究

夏鑫钰¹,耿莹²,叶宇³,李璐⁴,王晓茜²,孙颖⁵,徐艳⁵

1. 江苏省口腔医院
2. 南京医科大学附属口腔医院
3. 南京医科大学口腔研究所，南京医科大学附属口腔医院牙周科
4. 南京医科大学口腔疾病研究江苏省重点实验室；南京医科附属口腔医院牙周科
5. 南京医科大学附属口腔医院牙周科

收稿日期:2015-03-24 修回日期:2015-03-26 出版日期:2015-06-28 发布日期:2015-07-01
通讯作者: 徐艳 E-mail:yanxu@njmu.edu.cn
基金资助:
国家自然科学基金;江苏省高校优势学科建设工程项目

Association between interleukin-17 polymorphisms and aggressive periodontitis patients

Received:2015-03-24 Revised:2015-03-26 Online:2015-06-28 Published:2015-07-01

摘要/Abstract

摘要： 　目的　已有少量文献证实白细胞介素-17（interleukin-17,IL-17）基因多态性与多种免疫炎症性疾病相关。本研究旨在探索中国汉族人群中，IL-17A(rs2275913)和IL-17F(rs763780)位点单核苷酸多态性（Single nucleotide polymorphisms，SNPs）与侵袭性牙周炎(aggressive periodontitis,AgP)的相关性。方法　采集87例AgP、79例牙周健康者的外周静脉血，提取基因组DNA，采用多重SNaPshot技术分析待测位点的基因多态性。结果　IL-17A(rs2275913)位点的基因型分布、IL-17F(rs763780)位点的基因型和等位基因分布在AgP组和健康对照(healthy controls,C)组中差异均无统计学意义（P≥0.05）。IL-17A(rs2275913)位点在AgP组中A等位基因频率显著高于健康对照组（54.0% vs.42.4%，P=0.034，OR=1.596,95%CI =1.034-2.463）。结论　研究结果显示IL-17A(rs2275913) 位点A等位基因可能是AgP发病的危险因素，提示IL-17A(rs2275913) 位点的多态性可能与中国汉族人群的AgP易感性有关。

关键词: 白细胞介素-17, 基因多态性, 侵袭性牙周炎

Abstract: Objective Interleukin-17 (IL-17) polymorphisms have been testified by a few studies to be associated with several immune inflammatory disease. The aim of present study was to explore whether the single nucleotide polymorphisms (SNPs) in IL-17A (rs2275913) and IL-17F (rs763780) genes were correlated with the susceptibility to aggressive periodontitis (AgP) in Chinese Han population. Methods 87 AgP patients and 79 healthy controls were recruited in this study. Genomic DNA was extracted from the peripheral blood samples, and gene polymorphisms were determined by multiplex SNaPshot technique. Results No significant differences were observed in genotype or allele frequency distributions of IL-17A (rs2275913) and IL-17F (rs763780) between AgP and healthy controls (P≥0.05). However, the A allele frequencies of IL-17A (rs2275913) were significantly higher in AgP patients compared with healthy controls (54.0% vs.42.4%, P=0.034, OR=1.596, 95%CI=1.034-2.463). Conclusion These findings indicate that the IL-17A (rs2275913) polymorphism seemed to be a genetic risk factor of AgP. It suggests that the IL-17A(rs2275913) polymorphism may be associated with the susceptibility to AgP in Chinese Han population.

Key words: Interleukin-17, Polymorphisms, Aggressive periodontitis

中图分类号:

R781.42

夏鑫钰耿莹叶宇李璐王晓茜孙颖徐艳. 白细胞介素-17基因多态性与侵袭性牙周炎的相关性研究[J]. 口腔医学, 2015, 35(6): 417-420.

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白细胞介素-17基因多态性与侵袭性牙周炎的相关性研究

Association between interleukin-17 polymorphisms and aggressive periodontitis patients

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