生长激素对大鼠牙齿移动及牙周组织影响的研究

摘要/Abstract

摘要： ［摘要］目的研究生长激素（growth hormone,GH）对大鼠牙周膜、牙槽骨和正畸牙齿移动的影响。方法 40只7周龄Wistar雄性大鼠，随机分成对照组（C）和实验组（E），每组20只。实验组每只大鼠每天接受腹部皮下注射GH0.15 IU/(kg·d); 对照组每天接受等量的生理盐水注射。所有大鼠的上切牙和左侧上颌第一磨牙间放置一力值0.49 N镍钛拉簧，以使第一磨牙向近中移动。加力后3、7、10和14 d处死大鼠。游标卡尺测量第一磨牙移动的距离。上颌骨切片做HE染色。结果 10 d和14 d时，实验组和对照组相比，牙齿移动距离有显著差异（P<0.05)。与对照组相比，实验组在7 d有更多的破骨细胞，同时在7和10 d表现出更多的血管。结论短期注射GH会影响骨改建及加快正畸牙齿移动，缩短治疗时间。

关键词: 关键词：生长激素, 牙齿移动, 苏木素-伊红染色, 正畸

Abstract: Abstract: Objective To investigate the effect of growth hormone (GH) on periodontal ligament,alveolar bone and orthodontic tooth movement in rats.Methods 40 Wistar rats (gender:male; age:7weeks) were randomly divided into control and experimental groups,each having 20 rats.The experimental group received subcutaneous injections of growth hormone at a dose of 0.15 IU/kg·d,and the control group received equivalent volumes of saline.A nickel titanium spring was fixed to maxillary incisors and the left upper first molar,with a force of 0.49N,so as to move the molar mesially.The rats were sacrificed on day 3,7,10 and 14.The distance of tooth movement was determined by using a vernier caliper.The sections of the maxillae were prepared for hematoxylineosin staining.Results On day 10 and 14,there was a significant difference of total tooth movement between the experimental group and the control group (P <0.05).Compared with the control group,the experimental group showed more osteoclasts on day 7 and more blood vessels on day 7 and 10.Conclusions Shortterm GH administration can affect bone remodeling and accelerate orthodontic tooth movement,shortening treatment time.

Key words: Keywords: Growth hormone, Tooth movement, Hematoxylin-eosin staining, Orthodontics

中图分类号:

R783.5

鞠华龙安晶涛杨莉苹孙婷婷唐林. 生长激素对大鼠牙齿移动及牙周组织影响的研究[J]. 口腔医学, 2015, 35(7): 542-545.

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