齿垢密螺旋体双组分调控系统的研究进展

摘要/Abstract

摘要： ［摘要］口腔密螺旋体是健康牙周人群口腔细菌的成员之一。但牙周袋菌群一旦失调，密螺旋体会大肆繁殖并成为牙周疾病的主要致病菌。目前，关于密螺旋体适应口腔微生态环境并介导牙周疾病的分子机制广泛引起研究学者的青睐。研究密螺旋体的基因序列和双组分调控系统可能为解释上述现象提供了理论依据。因此，本综述将集中讨论齿垢密螺旋体基因组学中双组分调控系统的特征，以期为寻找控制齿垢密螺旋体繁殖、治疗牙周疾病的新靶点和新药物提供一定的理论基础。

关键词: 密螺旋体, 牙周疾病, 基因组学, 双组分调控系统, 综述

Abstract: Abstract： Oral treponema is a member of oral bacteria in the healthy periodontal tissue.However,once flora imbalance occurs in the periodontal pocket,oral treponema will reproduce rapidly and becomes the main pathogenic bacteria of periodontal disease.Currently,researchers all focus on how treponema can adapt to oral micro ecological environment and mediate periodontal disease.Studying the gene sequence of treponema and twocomponent regulatory systems may provide the theoretical basis for explaining the above phenomena.Therefore,this review will focus on the characteristics of twocomponent regulatory systems of treponema,so as to provide certain theoretical basis for controlling breeding of treponema denticola and finding new targets or new therapeutic agents in the treatment of periodontal disease.

Key words: treponema, periodontal disease, genomics, two-component regulatory systems, review

中图分类号:

R781.4

周素平. 齿垢密螺旋体双组分调控系统的研究进展[J]. 口腔医学, 2015, 35(7): 605-608.

参考文献

[1] Dye BA, Tan S, Smith V, et al. Trends in oral health status: United States, 1988-1994 and 1999-2004[J]. Vital Health Stat 11,2007,(248):1-92.
[2] Darveau RP. Periodontitis: a polymicrobial disruption of host homeostasis[J]. Nat Rev Microbiol,2010,8(7):481-490.
[3] Paster BJ, Olsen I, Aas JA, et al. The breadth of bacterial diversity in the human periodontal pocket and other oral sites[J]. Periodontol 2000,2006,42:80-87.
[4] Kshirsagar AV, Offenbacher S, Moss KL, et al. Antibodies to periodontal organisms are associated with decreased kidney function. The Dental Atherosclerosis Risk In Communities study[J]. Blood Purif,2007,25(1):125-132.
[5] Chen YW, Umeda M, Nagasawa T, et al. Periodontitis may increase the risk of peripheral arterial disease[J]. Eur J Vasc Endovasc Surg,2008,35(2):153-158.
[6] Makiura N, Ojima M, Kou Y, et al. Relationship of Porphyromonas gingivalis with glycemic level in patients with type 2 diabetes following periodontal treatment[J]. Oral Microbiol Immunol, 2008,23(4):348-351.
[7] Kavoussi SK, West BT, Taylor GW, et al. Periodontal disease and endometriosis: analysis of the National Health and Nutrition Examination Survey[J]. Fertil Steril,2009,91(2):335-342.
[8] Inaba H, Amano A. Roles of oral bacteria in cardiovascular diseases--from molecular mechanisms to clinical cases: Implication of periodontal diseases in development of systemic diseases[J]. J Pharmacol Sci,2010,113(2):103-109.
[9] Ellen RP, Galimanas VB. Spirochetes at the forefront of periodontal infections[J]. Periodontol 2000,2005,38:13-32.
[10] Radolf JD, Lukehart SA, editors. Pathogenic Treponema: molecular and cellular biology. Norfolk, UK: Caister Academic Press, 2006
[11] Samuels DS, Radolf JD, editors. Borrelia: molecular biology, host interaction and pathogenesis. Norfolk, UK:Caister Academic Press,2010.
[12] 卢思贤,王青. 齿垢密螺旋体的研究进展[J]. 国际口腔医学杂志,2013,40(1):121-124.
[13] Ruby JD, Lux R, Shi W, et al. Effect of glucose on Treponema denticola cell behavior. Oral Microbiol Immunol,2008,23(3):234-238.
[14] Seshadri R, Myers GS, Tettelin H, et al. Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes[J]. Proc Natl Acad Sci U S A,2004,101(15): 5646-5651.
[15] McHardy I, Keegan C, Sim JH, et al. Transcriptional profiles of Treponema denticola in response to environmental conditions[J]. PLoS One,2010,5(10):e13655.
[16] Galperin MY. Structural classification of bacterial response regulators: diversity of output domains and domain combinations[J]. J Bacteriol,2006,188(12):4169-4182.
[17] Galperin MY, Nikolskaya AN, Koonin EV. Novel domains of the prokaryotic two-component signal transduction systems[J]. FEMS Microbiol Lett,2001,203(1):11-21.
[18] Frederick JR, Rogers EA, Marconi RT. Analysis of a growth-phase-regulated two-component regulatory system in the periodontal pathogen Treponema denticola[J]. J Bacteriol,2008,190(18): 6162-6169.
[19] Sarkar J, Frederick J, Marconi RT. The Hpk2-Rrp2 two-component regulatory system of Treponema denticola: a potential regulator of environmental and adaptive responses[J]. Mol Oral Microbiol,2010,25(4):241-251.
[20] Ohta N, Newton A. The core dimerization domains of histidine kinases contain recognition specificity for the cognate response regulator[J]. J Bacteriol,2003,185(15):4424-4431.
[21] Nikolskaya AN, Galperin MY. A novel type of conserved DNA-binding domain in the transcriptional regulators of the AlgR/AgrA/LytR family[J]. Nucleic Acids Res,2002,30(11): 2453-2459.
[22] M?glich A, Ayers RA, Moffat K. Structure and signaling mechanism of Per-ARNT-Sim domains[J]. Structure,2009,17(10):1282-1294.
[23] Revel AT, Talaat AM, Norgard MV. DNA microarray analysis of differential gene expression in Borrelia burgdorferi, the Lyme disease spirochete[J]. Proc Natl Acad Sci U S A,2002,99(3): 1562-1567.
[24] Ojaimi C, Brooks C, Casjens S, et al. Profiling of temperature-induced changes in Borrelia burgdorferi gene expression by using whole genome arrays[J]. Infect Immun,2003,71(4):1689- 1705.
[25] Lai Y, Chu L. Novel mechanism for conditional aerobic growth of the anaerobic bacterium Treponema denticola[J]. Appl Environ Microbiol,2008,74(1):73-79.
[26] Phillips ZE, Strauch MA. Bacillus subtilis sporulation and stationary phase gene expression [J]. Cell Mol Life Sci,2002,59(3):392-402.
[27] Hager J, Staker BL, Bugl H, et al. Active site in RrmJ, a heat shock-induced methyltransferase[J]. J Biol Chem,2002,277(44):41978-41986.
[28] Maki S, Kornberg A. DNA polymerase III holoenzyme of Escherichia coli. II. A novel complex including the gamma subunit essential for processive synthesis[J]. J Biol Chem,1988, 263(14):6555-6560.
[29] Mohammadi T, Karczmarek A, Crouvoisier M, et al. The essential peptidoglycan glycosyltransferase MurG forms a complex with proteins involved in lateral envelope growth as well as with proteins involved in cell division in Escherichia coli[J]. Mol Microbiol,2007,65(4): 1106-1121.