关键词: 生长分化因子15, 过表达, 慢病毒载体

Abstract: Objective To construct lentiviral vectors of GDF15 gene overexpression.Methods Based on the mRNA sequence of GDF15 gene,the GDF15 gene was made.The overexpression vectors were constructed and transfected into competent cells which were detected by sequencing.The 293T cells were transfected by the recombinant lentiviral vectors to achieve the lentivirus packaging production.The lentiviruses were transfected into 293T cells.The expression of GDF15 gene in 293T cell was detected by Western blot in order to determine the validity of GDF15 gene overexpression.Results It was confirmed by sequencing that the GDF15 gene was correctly inserted into the vectors,and that the GDF15 gene overexpressed lentiviral vectors were successfully constructed.After the lentiviruses transfected 293T cells,the expression of GDF15 gene increased significantly at protein level.Conclusions The lentiviral vectors of GDF15 gene overexpression were constructed successfully,and they efficiently up-regulated the expression of GDF15 in 293T cells.

Key words: GDF15, Over-expression, Lentiviral vector