MicroRNA27b 通过靶向调控FZD7抑制口腔鳞状细胞癌细胞的增殖

摘要/Abstract

摘要： 目的探讨microRNA27b （miR27b）对口腔鳞状细胞癌细胞增殖的影响及其作用的分子生物学机制。方法 MTT方法检测细胞增殖水平；实时定量PCR方法检测miR27b表达水平以及卷曲蛋白7（Frizzled7，FZD7）、cyclin D1和cmyc的信使RNA（mRNA）表达水平；Western blot方法检测FZD7的蛋白表达水平；荧光素酶报告基因方法检测miR27b与FZD7的靶向关系以及Wnt信号通路的活性。结果 miR27b在口腔鳞状细胞癌细胞株中表达降低；过表达miR27b可以显著抑制口腔鳞状细胞癌细胞的增殖；miR27b可以靶向FZD7并抑制FZD7的表达及其下游的Wnt信号通路。结论 miR27b通过靶向抑制FZD7的表达来抑制口腔鳞状细胞癌细胞的增殖，其机制可能与抑制FZD7介导的Wnt信号通路的激活有关。

关键词: 口腔鳞状细胞癌, FZD7, miR27b, 细胞增殖

Abstract: Objective To investigate the role of microRNA27b (miR27b) in regulating the cell proliferation of oral squamous cell carcinoma and the underlying molecular mechanism. Methods Cell proliferation was measured by MTT assay. The expression of miR27b, the mRNA expression levels of Frizzled7 (FZD7), cyclin D1 and cmyc were detected by realtime quantitative polymerase chain reaction (qPCR). The protein expression of FZD7 was detected by Western blot analysis. The targeted relationship between miR27b and FZD7, and the activity of Wnt signaling pathway were examined by luciferase reporter assay. Results The expression of miR27b in oral squamous cell carcinoma cell lines was significantly decreased. Overexpression of miR27b markedly inhibited the cell proliferation of oral squamous cell carcinoma. miR27b could target and inhibit FZD7 expression and suppress Wnt signaling pathway. Conclusions miR27b could inhibit the cell proliferation of oral squamous cell carcinoma through targeting and inhibiting FZD7 and FZD7mediated Wnt signaling pathway.

Key words: oral squamous cell carcinoma, FZD7, miR27b, cell proliferation

中图分类号:

R739.8

刘秉尧张森林曹罡董震陈伟徐金科. MicroRNA27b 通过靶向调控FZD7抑制口腔鳞状细胞癌细胞的增殖[J]. , 2016, 36(3): 200-205.

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