SLA种植体表面干扰LRP5信号影响骨结合的实验研究

›› 2016, Vol. 36 ›› Issue (4): 309-313.

SLA种植体表面干扰LRP5信号影响骨结合的实验研究

罗善峰¹,方文²,杨国利²,赵士芳²

1. 浙江省绍兴市口腔医院
2. 浙江大学医学院附属口腔医院

收稿日期:2015-11-11 修回日期:2016-01-11 出版日期:2016-04-28 发布日期:2016-04-24
通讯作者: 赵士芳 E-mail:muye868@163.com
基金资助:
国家自然基金;浙江省自然基金

The effect of shLPR5 on the osseointegration on SLA-treated titanium implant surface

Received:2015-11-11 Revised:2016-01-11 Online:2016-04-28 Published:2016-04-24
Contact: Fang ShiZhao E-mail:muye868@163.com

摘要/Abstract

摘要： 目的通过构建干扰型LRP5慢病毒（LV-LRP5），评估LRP5基因的干扰对大颗粒喷砂酸蚀表面处理（SLA）的种植体表面骨结合的影响。方法种植体表面进行SLA处理，构建LV-LRP5慢病毒载体，通过转染骨髓基质干细胞（BMSCs）检测转染效果。SLA表面处理种植体分为两组：LV-LRP5组和空白组。LV-LRP5的种植体周围注射shRNA病毒液，空白组常规种植。6周后取含有种植体的组织块进行硬组织切片，HE染色观察和评估新骨的形成，测量种植体-骨接触率和种植体螺纹内的骨密度来评价成骨的效果。结果 LV-LRP5能稳定转染BMSCs。种植体植入6周后，空白组种植体与新骨之间的接触较广泛，而LV-LRP5组种植体与新骨之间的接触较局限。LV-LRP5组的种植体-骨接触率及螺纹内骨密度均较空白组低，经统计学分析，两组在6周时具有显著性差异（P＜0.05）。结论 SLA种植体表面干扰LRP5降低了种植体骨结合，该过程可能通过降低Wnt/LRP5信号系统或联结其他成骨信号系统进行。

关键词: LRP5, SLA种植体表面处理, 骨结合

Abstract: Objective To investigate the effect of shLRP5 on the osseointegration on SLA-treated titanium implant surface by constructing shLRP5 lentivirus vetor. Methods One target gene, LRP5, was testified to be involved in the SLA-treated-peri-implant bone formation. The implants were separated into two groups:LV-LRP5 group and blank group. Twenty implants were inserted into tibias of ten rats. We injected shLRP5 lentivirus vetor around implants of LV-LRP5 group. The effect of the SLA surface on peri-implant bone formation was evaluated by histomorphometric analysis. Bone to implant contact (BIC) and bone area (BA) were measured and statistically analyzed. Results LV-LRP5 transfected BMSCs stably. After 6 weeks of implantation, the implant bone contact rate and bone density in the LV-LRP5 group were lower than those in the blank group. Both two groups had significant difference (P＜0.05). Conclusion shLRP5 decreased the osseointegration on SLA-trated titanium implant. The mechanism of shLRP5 that decreased the osseointegration on SLA-trated titanium implant might be the decrease of Wnt/LRP5 pathway or cross talk with other pathways.

Key words: LRP5, SLA treatment, osseointegration

中图分类号:

R349.69

罗善峰方文杨国利赵士芳. SLA种植体表面干扰LRP5信号影响骨结合的实验研究[J]. , 2016, 36(4): 309-313.

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