外周血来源的间充质干细胞与内皮祖细胞体外构建血管化组织的研究

›› 2016, Vol. 36 ›› Issue (4): 318-323.

外周血来源的间充质干细胞与内皮祖细胞体外构建血管化组织的研究

刘俊¹,杜月明¹,徐阳²,章非敏¹,陈刚¹

1. 南京医科大学口腔医学院
2. 南京医科大学附属口腔医院

收稿日期:2015-12-18 修回日期:2016-01-08 出版日期:2016-04-28 发布日期:2016-04-24
通讯作者: 章非敏 E-mail:fmzhang@njmu.edu.cn
基金资助:
江苏高校优势学科建设工程资助项目;江苏省医学领军人才

Study of vascularization in vitro with peripheral blood-derived mesenchymal stem cells and endothelial progenitor cells

Received:2015-12-18 Revised:2016-01-08 Online:2016-04-28 Published:2016-04-24

摘要/Abstract

摘要： 目的利用两种外周血细胞在体外构建出血管样组织。方法从外周血提取内皮祖细胞与间充质干细胞并鉴定，在纤维蛋白凝胶中以不同的密度培养,在显微镜下观察至第7天。结果兔外周血可以提取出内皮祖细胞与间充质干细胞，内皮祖细胞与间充质干细胞的比值为100万/mL：200万/mL时可以在体外形成血管样结构。结论外周血中可以提取出特定细胞并在体外共培养形成血管样组织。

关键词: 外周血内皮祖细胞, 外周血间充质干细胞, 纤维蛋白, 体外血管化

Abstract: Objective To engineer vascularized tissue with two types of cells from peripheral blood. Methods Endothelial progenitor cells (EPC) and peripheral blood-derived mesenchymal stem cells (PBMSC) were isolated from rabbit peripheral blood and identified. Then, they were cultured in fibrin at different ratios under the observation of a microscope until the 7th day after the initial creation. Results EPC and PBMSC were successfully isolated from rabbit peripheral blood. Vascularized tissue were acquired at a certain ratio (EPC were 1 million cells/mL, PBMSC were 2 million cells/mL). Conclusion Certain cells isolated from peripheral blood can be used to acquire vascularized tissue by co-cultured strategy at a certain density in vitro.

Key words: EPC, PBMSC, fibin, vascularization in vitro

中图分类号:

R394.26

刘俊杜月明徐阳章非敏陈刚. 外周血来源的间充质干细胞与内皮祖细胞体外构建血管化组织的研究[J]. , 2016, 36(4): 318-323.

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