Axl基因在口腔鳞状细胞癌中的表达及意义

›› 2016, Vol. 36 ›› Issue (4): 333-336.

Axl基因在口腔鳞状细胞癌中的表达及意义

王俊杰¹,赵腾达²,刘兴光¹,朱王勇¹,梁培盛¹,陶谦³

1. 中山大学光华口腔医学院·附属口腔医院口腔颌面外科，广东省口腔医学重点实验室
2. 山东大学附属省立医院口腔颌面外科
3. 中山大学光华口腔医学院·附属口腔医院口腔颌面外科

收稿日期:2015-11-12 修回日期:2016-01-06 出版日期:2016-04-28 发布日期:2016-04-24
通讯作者: 陶谦 E-mail:taoqian@mail.sysu.edu.cn
基金资助:
广东省自然科学基金;广州市科技计划项目

Expression and clinical significance of the Axl gene in oral squamous cell carcinoma

Received:2015-11-12 Revised:2016-01-06 Online:2016-04-28 Published:2016-04-24

摘要/Abstract

摘要： 目的检测Axl（Anexelekto）在口腔鳞状细胞癌中的表达，探索Axl与口腔鳞状细胞癌发生、发展的关系。方法免疫组织化学法（IHC）检测42例口腔鳞状细胞癌患者的癌组织（口腔癌组）及其相应的癌旁组织（癌旁组）Axl的表达状况。原代培养正常人口腔上皮细胞（HOEC）,并培养1株永生化正常口腔角化上皮细胞系（NOK）及2株人舌鳞状细胞癌细胞系（UM1、UM2）。实时荧光定量聚合酶链反应（qRT-PCR）和蛋白免疫印迹分析（Western blot）检测上述4种细胞中的Axl的mRNA和蛋白的表达情况。结果 Axl在口腔鳞状细胞癌组织中的阳性表达率（61.9%）高于其相应的癌旁正常组织（26.2%），差异具有统计学意义（P＜0.05），qRT-PCR和Western blot结果示在HOEC、NOK、UM2、UM1中Axl基因的mRNA表达和蛋白表达逐步增高，且在UM2、UM1中明显高于HOEC、NOK。结论 Axl在口腔癌组织及口腔癌细胞株中的表达较口腔正常组织和正常口腔上皮细胞者中高表达，提示Axl基因可能与口腔鳞状细胞癌的发生、发展相关。

关键词: Axl, 口腔鳞状细胞癌, 表达

Abstract: Objective To investigate the relationship between the expression of Axl gene and oncogenesis of oral squamous cell carcinoma (OSCC). Methods The immunohistochemical expressions of Axl genes in tumor tissues from 42 patients with OSCC (OSCC group) and in correspondingly adjacent tissues (adjacent group) were examined. In vitro, expression of mRNA and protein of Axl in normal human oral epithelium cell (HOEC), immortalized normal oral keratinocyte cell (NOK), tongue squamous cell carcinoma cell lines UM1 and UM2 were detected by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR) and Western blot (WB), respectively. Results There was significant difference between OSCC group and adjacent group (61.9% vs. 26.2%, P＜0.05) in immunohistochemical expression of Axl. Expression of mRNA and proteins of Axl in UM2 and UM1 were significantly higher than those in HOEC and NOK in vitro. Conclusions Strongly expressed in OSCC tissue and cell lineages compared with their normal counterparts, Axl gene may play a role in OSCC oncogenesis.

Key words: Axl, OSCC, expression

中图分类号:

R739.8

王俊杰赵腾达刘兴光朱王勇梁培盛陶谦. Axl基因在口腔鳞状细胞癌中的表达及意义[J]. , 2016, 36(4): 333-336.

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