壳寡聚糖抑制变形链球菌的致龋能力

›› 2016, Vol. 36 ›› Issue (7): 587-590.

壳寡聚糖抑制变形链球菌的致龋能力

鞠铎¹,关晓旭²,王慧明²

1. 湖州市中心医院
2. 浙江大学附属口腔医院

收稿日期:2015-11-12 修回日期:2016-01-06 出版日期:2016-07-28 发布日期:2016-07-19
通讯作者: 王慧明 E-mail:huimingwang1960@163.com
基金资助:
变异链球菌frp基因对牙菌斑生物膜致龋性影响的实验研究

Inhibition of chitooligosaccharide on the cariogenicity of streptococcus mutans

Received:2015-11-12 Revised:2016-01-06 Online:2016-07-28 Published:2016-07-19

摘要/Abstract

摘要： 目的研究壳寡聚糖（chitooligosaccharide，COS）对变形链球菌生长、产酸及粘附能力的影响。方法选用变形链球菌标准株ATCC 25175，采用对倍稀释法测定COS对变形链球菌的最低抑菌浓度（minimum inhibitory concentration，MIC）和最低杀菌浓度（minimum bactericidal concentration，MBC）；采用菌落计数法，通过时间—杀菌曲线的变化，分析COS对细菌生长速度的影响；将COS及变形链球菌菌悬液各1 mL接种于无菌试管内厌氧培养，使COS的终浓度分别达到1/2 MIC、1/4 MIC、1/8 MIC，测定上清液pH变化并绘制pH曲线；采用液体闪烁计数法测定壳寡聚糖对变形链球菌粘附羟磷灰石能力的影响。结果 COS对变形链球菌的MIC和MBC分别为2.00 g/L和4.00 g/L；COS能有效抑制变形链球菌的生长，较高浓度时对细菌还具有杀菌作用。亚抑菌浓度的COS可显著影响变形链球菌的产酸性和粘附能力，并且这种抑制作用呈浓度依赖性。结论 COS对变形链球菌的生长、产酸和粘附能力均有抑制作用。

关键词: 变形链球菌, 壳寡聚糖, 生长, 产酸, 粘附

Abstract: Objective To investigate the effect of chitooligosaccharide (COS) on the growth, acid production and adhesion of streptococcus mutans(S. mutans). Methods Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of COS against S. mutans(ATCC 25175) were determined by microdilution technique. The influence of COS on the growth rate of S. mutans was assessed by timekill curve. To measure the impact of COS on acid production of S. mutans, 1 mL bacterial cell suspension containing 1/2 MIC, 1/4 MIC or 1/8 MIC COS was seeded in the sterile culture tubes, and pH value in the collected supernatant was analyzed and pH curve was then figured out. With liquid scintillation counting method, the adverse effect of COS on the bacterial adhesion ability to hydroxylapatite was also fully investigated. Results COS inhibited S. mutans growth with MIC at 2.00 g/L and MBC at 4.00 g/L, respectively. At low concentration, COS exhibited an inhibitory effect on S. mutans growth, while at high concentration, COS was capable of directly killing bacteria. 2.00 g/L (MIC) COS could significantly reduce the acid production and adhesion of S. mutans and this inhibitory effects was in concentrationdependent manners. Conclusion COS can inhibit the growth, acid production and adhesion of S. mutans.

Key words: streptococcus mutans, chitooligosaccharide, growth, acid production, adherence

中图分类号:

R780.1

鞠铎关晓旭王慧明. 壳寡聚糖抑制变形链球菌的致龋能力[J]. , 2016, 36(7): 587-590.

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