牵张应变诱导人牙周膜细胞凋亡信号通路初探

›› 2016, Vol. 36 ›› Issue (7): 599-602.

牵张应变诱导人牙周膜细胞凋亡信号通路初探

郝轶,李晶,李隽,马佳音

上海交通大学医学院附属第九人民医院

收稿日期:2015-06-04 修回日期:2015-07-05 出版日期:2016-07-28 发布日期:2016-07-19
通讯作者: 马佳音 E-mail:gangnin@sina.com

Study on stretching forceinduced apoptosis mechanism of human periodontal ligament cells

Received:2015-06-04 Revised:2015-07-05 Online:2016-07-28 Published:2016-07-19

摘要/Abstract

摘要： 目的通过研究Caspase酶在牵张应变作用下的变化，来初步探讨其引起人牙周膜细胞（HPDLCs）凋亡的可能机制。方法体外培养HPDLCs，利用动态机械应变细胞加载装置对HPDLCs加载1%、10%和20%的动态牵张应变6、12、24、48 h，利用酶标仪检测Caspase3活性以及添加Caspase8和Caspase9阻断剂后Caspase3活性。结果 Caspase3活性具有一定的时间和应变值依赖性，各应变组细胞的Caspase3活性在加载24 h时达到峰值，Caspase9阻断剂可以显著降低Caspase3的活性，而Caspase8却并没有明显作用同时发现。结论 Caspase9激活Caspase3介导的凋亡通路参与了牵张应变诱导HPDLCs的凋亡。

关键词: 牵张应变, 凋亡, Caspase酶, 人牙周膜细胞

Abstract: Objective To explore the apoptosis mechanism of human periodontal ligament cells (HPDLCs) induced by Caspase under stretching force. Methods HPDLCs were cultured in vitro, and cells were stretched by dynamic mechanical strain 1%, 10% and 20% for 6 h, 12 h, 24 h and 48 h. The Caspase3 activity was measured after stretching in PDL cells that were treated with a specific inhibitor of either Caspase8 or Caspase9 prior to the application of stretching force. Results The activity of Caspase3 increased substantially in a time and force dependent manner after the application of a cyclic stretching force. It reached a peak at 24 h. The Caspase9 inhibitor abolished the stretching forceinduced activation of Caspase3, whereas the Caspase8 inhibitor demonstrated no effect. Conclusion Cyclic stretching force can induce apoptosis in HPDLCs through the activation of Caspase3 via the Caspase9 signaling cascade.

Key words: stretching force, apoptosis, Caspase, HPDLCs

中图分类号:

R781.4

郝轶李晶李隽马佳音. 牵张应变诱导人牙周膜细胞凋亡信号通路初探[J]. , 2016, 36(7): 599-602.

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