槲皮素对LPS刺激的人牙周膜细胞表达IL-6的影响

摘要/Abstract

摘要： 目的研究槲皮素对接受大肠杆菌脂多糖(lipopolysaccharide,LPS)刺激的人牙周膜细胞（human periodontal ligament cells,hPLDCs）表达IL6（interleukin6）的影响,探讨槲皮素（quercetin,Que）的抗炎作用。方法使用酶消化组织块法培养人牙周膜细胞并鉴定，根据ELISA(enzymelinked immunosorbent assay，酶联免疫吸附实验)法测定不同时间点以及不同浓度下槲皮素对人牙周膜细胞抑制LPS 诱导细胞表达IL6的作用。结果 LPS刺激24 h左右时人牙周膜细胞分泌IL6达到峰值，而槲皮素处理后的细胞再加入LPS刺激所产生的IL6与单纯的LPS组相对比明显降低，有统计学意义。经不同浓度槲皮素处理细胞后加入LPS刺激所产生的IL6无统计学差异。结论槲皮素可以抑制LPS 诱导的人牙周膜细胞表达IL6,提示槲皮素可能通过某些途径调节影响IL6的合成和分泌,因而在炎症的早期发挥其减轻炎症反应的作用。

关键词: 脂多糖, 酶联免疫吸附实验, 人牙周膜细胞, 槲皮素, 白介素6

Abstract: Objective To observe the effects of quercetin on the expression of IL6 (interleukin6) by LPS (lipopolysaccharide) induced human periodontal ligament cells (hPDLCs), and to explore the antiinflammatory action of quercetin (Que). Methods hPDLCs were cultured in enzymic digestion tissue blocks and then identified. The inhibitory effects of quercetin with different concentrations on the expression of IL6 by LPSstimulated hPLDCs at different time points were assayed by ELISA (enzymelinked immunosorbent assay). Results IL6 secreted by hPLDCs peaked after the stimulation of LPS for 24 h. Compared with the LPS group, however, IL6 expressed by cells treated with quercetin before the stimulation of LPS significantly decreased with a statistical significance. No significant difference was found in different concentrations of quercetin groups. Conclusion Quercetin can inhibit the LPSinduced expression of IL6 in hPDLCs, suggesting that it may regulate the synthesis and secretion of IL6 in some way, thus alleviating inflammatory reaction in the early stage of inflammation.

Key words: lipopolysaccharide, enzymelinked immunosorbent assay, human periodontal ligament cells, quercetin, interleukin6

中图分类号:

R780.2

任谞挺徐俊峰金亚峰马益欣. 槲皮素对LPS刺激的人牙周膜细胞表达IL-6的影响[J]. , 2016, 36(7): 603-606.

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