磨牙缺失对幼年大鼠海马神经元凋亡及casepase-3蛋白表达的影响

›› 2017, Vol. 37 ›› Issue (1): 11-15.

磨牙缺失对幼年大鼠海马神经元凋亡及casepase-3蛋白表达的影响

于璐,刘奕杉,李伯琦,李宇,郑丽珠

新疆乌鲁木齐市新市区新疆医科大学第一附属医院

收稿日期:2016-08-02 修回日期:2016-09-09 出版日期:2017-01-28 发布日期:2017-01-17
通讯作者: 刘奕杉 E-mail:lys-tree@126.com
基金资助:
Wnt5a/miRNA-335在牙萌出中调控牙囊破骨、成骨细胞成熟的分子机制;幼年大鼠磨牙缺失后对中枢海马组织损伤的研究;磨牙缺失对幼年大鼠海马区细胞凋亡的研究

Effect of molar-missing on hippocampus nerve cell apoptosis and casepase-3 protein ecpression in young rats.

Received:2016-08-02 Revised:2016-09-09 Online:2017-01-28 Published:2017-01-17

摘要/Abstract

摘要： 目的　观察磨牙缺失后对幼年大鼠海马CA1区神经元细胞凋亡及海马神经元中Caspase3的表达的影响，以探讨磨牙缺失幼年大鼠海马神经细胞凋亡可能的机制。方法　雄性SD大鼠，4~5周龄60只，按完全随机设计平均分成对照组、实验组（磨牙缺失组）（n=30），再依据造模后断头取脑时间将30只大鼠随机分成4周组、8周组、20周组，采用HE染色及免疫组织化学法检测Caspase3蛋白的表达情况，及末端标记法（TUNEL）法观察大鼠脑内神经细胞凋亡的变化。结果　①对照组海马CA1区细胞形态结构正常，无明显病理性改变。实验组细胞出现肿胀、空泡，结构排列紊乱，神经元细胞明显减少。②在4周、8周、20周，对照组Caspase3无统计学差异（P=0.301），实验组海马CA1区Caspase3阳性细胞数增加，差异有统计学意义（P=0.000），任意两组相比Caspase3表达差异均有统计学意义，P＜0.001;③实验组在不同时间段TUNEL阳性细胞数有统计学差异（P=0.000），对照组无统计学差异（P=0.276）；两组相比表达差异有统计学意义（P＜0.001）。结论　长时期咀嚼刺激减少会导致大鼠海马区细胞凋亡增多，同时可上调Caspase3蛋白的表达，提示长时期磨牙缺失诱导加快致发育期的大鼠海马组织细胞发生凋亡。

关键词: 关键词：牙齿缺失, 学习记忆, 海马组织, 细胞凋亡

Abstract: Abstract: Objective To observe the influence of molar-missing on apoptosis of hippocampus CA1 neurons and expression of casepase-3 in hippocampus neurons of young rats ,and to investigate the possible mechanism of juvenile molar-missing on hippocampus neural cell apoptosis in young rats. Methods 60 male SD rats(at the age of 4~5 weeks) were randomly divided into control group and experimental group, 30 in each group .And then 30 rats were randomly divided into three groups(4-week group, 8-week group and 20-week group) based on when the modeling ends down to observe the expression of casepase-3 protein by using HE staining and immunohistochemistry, and to observe the dynamic changes of nerve cell apoptosis in rat hippocampus by using TUNEL method. Results: 1. The morphology and structure of hippocampus CA1 cells were normal in the control group, and there were no significant pathological changes. The cells in the experimental group were swollen, vacuoles appeared, the structural arrangement became disordered, and the neuron cells were significantly reduced.2. With time variation(4,8,20 weeks), positive cells in hippocampus CA1 region of experimental group by the number increased, and the difference was statistically significant (P = 0.000), while in the control group there was no statistical difference (P = 0.301) in casepase-3 expression. The differences were statistically significant in casepase-3 expression between any two groups (P < 0.001). 3.The TUNEL positive cells in experimental group had a statistically significant difference (P = 0.000). The TUNEL positive cells in control group had no significant difference (P = 0.276) . There was a statistically significant difference in the number of TUNEL positive cells between any two groups (P < 0.001).Conclusion: Longrun chewing stimulation decrease may increase apoptosis in hippocampus of rats and may increase the expression of casepase-3 protein at the same time. The results suggest that the long-term molar deletion in rat hippocampus induces the development of apoptosis of hippocampus tissue cells.

Key words: Key words : molar-missing, Learning and memory, Hippocampus tissue, Cell apoptosis

中图分类号:

R318

于璐刘奕杉李伯琦李宇郑丽珠. 磨牙缺失对幼年大鼠海马神经元凋亡及casepase-3蛋白表达的影响[J]. , 2017, 37(1): 11-15.

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