Osterix在小鼠牙本质生成和修复过程中的表达

›› 2017, Vol. 37 ›› Issue (11): 965-968.

Osterix在小鼠牙本质生成和修复过程中的表达

李功臣¹,胡凌玮²,许婷婷²,孙瑶²

1. 同济大学附属口腔医院
2. 同济大学口腔医学院

收稿日期:2017-04-24 修回日期:2017-05-23 出版日期:2017-11-28 发布日期:2017-11-24
通讯作者: 孙瑶 E-mail:Sunyao919@126.com

Expression of Osterix during Mice dentinogenesis and repairation

Received:2017-04-24 Revised:2017-05-23 Online:2017-11-28 Published:2017-11-24

摘要/Abstract

摘要： 目的：通过免疫组织化学方法观察小鼠牙本质生成过程中Osterix（OSX）的表达变化，探索其功能及意义。方法：取小鼠胚胎、不同年龄段及建立修复性牙本质模型的下颌骨，石蜡包埋切片后分别进行HE染色和免疫组织化学染色。结果：在钟状期晚期，OSX在牙尖处成牙本质细胞核内表达。小鼠出生后三天，OSX在牙冠的全部成牙本质细胞核内高表达。2周龄小鼠，冠部成牙本质细胞核内几乎不表达OSX，磨牙根部成牙本质细胞核内OSX高表达。牙尖磨损建模7天后，OSX在成牙本质细胞核内表达升高，14天时表达量下降。结论：OSX在促进成牙本质生成和修复过程中发挥作用。

关键词: Osterix, 成牙本质细胞, 牙本质生成, 修复性牙本质

Abstract: Objective : To explore the expression of Osterix during detinogenesis by the immunohistochemistry(IHC) staining. Methods: The mandibles of the mice were taken as specimens by HE and IHC after processing. Results: The results of immunohistochemistry showed that OSX expressed in the nucleus of odontoblast until the late bell stage. All odontoblasts under the crown express OSX at day 3. While IHC staining revealed that Osx rarely expressed in odontoblasts in the crown at day 14, but highly expressed in the root. OSX were highly expressed in odontoblasts after 7 days of operation, while were down regulated after 14days. Conclusion: It can be concluded that OSX protein may participate in the reparation of odontoblast, and have a role during dentinogenesis.

Key words: Osterix, Odontoblast, dentinogenesis, reparative dentin

李功臣胡凌玮许婷婷孙瑶. Osterix在小鼠牙本质生成和修复过程中的表达[J]. , 2017, 37(11): 965-968.

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