关键词: 煅烧骨, 富血小板纤维蛋白, 牙周骨缺损, BMP-2, OPG, RANKL

Abstract: OBJECTIVE: To observe expression and significance of bone morphogenetic protein-2(BMP-2),osteoprotegerin（OPG）and receptor activator of NFkappa B ligand（RANKL）protein in periodontal bone defects repair with calcined bone compounded with platelet－rich fibrin（PRF）in rabbits. METHODS: A total of 36 healthy rabbits were randomly divided into four groups, and unilateral periodontal tissue defect model was established in each group. These four groups were respectively treated with calcined bone, Bio-oss bone and calcined bone /PRF, and nothing (blank control group). After 4, 8 and 12 weeks, animals were sacrificed respectively for a complete specimen used in gross, Masson staining and Immunohistochemical observation. RESULTS AND CONCLUSION: Masson staining: Calcined bone, Bio-oss bone and calcined bone/PRF set of postoperative 4、8、12weeks the maturity of the new bone were higher than the control group. Calcined bone/PRF group after 8 weeks, visible part bright red when mature bone formation.Immunohistochemical observation: After 4 weeks, 8 weeks of calcined bone, Bio-oss and calcined bone/PRF BMP-2 expression was higher than control group,Statistically significant differences (P<0. 05). After 4 weeks of calcined bone/PRF group BMP-2, OPG strongly positive, and the number is higher than the other three groups, Statistically significant differences (P<0. 05). Expression of RANKL-positive cells 4 weeks were higher than 8 weeks, 12 weeks, reduction in bone remodeling process in varying degrees, Statistically significant differences (P<0. 05). Conclusion: Calcined bone/PRF set of repair of periodontal bone defects would increase the expression of BMP-2,OPG,RANKL, through the promotion of Osteoblast differentiation and stimulated OPG expression, Inhibiting osteoclast, help accelerate the periodontal bone remodeling, shorten the healing process.

Key words: Calcined bone, PRF, Periodontal bone defects , BMP-2, OPG, RANKL