CKIP-1对不同时间和部位松质骨影响的Micro-CT分析

›› 2017, Vol. 37 ›› Issue (3): 198-202.

CKIP-1对不同时间和部位松质骨影响的Micro-CT分析

秦东泽,胡奥,郭威孝,梁建飞,蒋威,许珊珊,曹强,张浚睿,陆斌,孔亮

陕西省西安市第四军医大学口腔医院颌面外科

收稿日期:2016-11-28 修回日期:2016-12-13 出版日期:2017-03-28 发布日期:2017-04-12
通讯作者: 孔亮 E-mail:kongliang@fmmu.edu.cn
基金资助:
CKIP-1通过抑制经典Wnt信号通路调控的BMMSCs成骨分化影响骨质疏松的机制研究

Micro-CT analysis of the effects of CKIP-1 on cancellous bone in different time and parts

Received:2016-11-28 Revised:2016-12-13 Online:2017-03-28 Published:2017-04-12

摘要/Abstract

摘要： ［摘要］目的探讨酪蛋白激酶2相互作用蛋白1（CKIP-1）对不同时间股骨和下颌骨松质骨变化的影响。方法取3个月和6个月CKIP-1基因敲除小鼠（CKIP-1 -/-）作为实验组，野生型小鼠（WT）作为对照组（n=5、同窝同性别、雄性），分别取股骨和下颌骨行MicroCT扫描，对其股骨和下颌骨松质骨进行定量分析，评估CKIP-1在不同骨发育阶段对不同部位骨量变化的影响。结果在3个月时，实验组较对照组股骨和下颌骨的BV/TV分别增加2.54％和1.69％，在6个月时分别增加3.77％和2.82％（P<0.05）。从3~6个月时，股骨和下颌骨的BV/TV分别下降了4.42％和1.1％（P<0.05）。结论股骨较下颌骨对CKIP1基因调控骨量更为敏感，随着时间变化也更加明显。

关键词: CKIP-1, Micro-CT, 松质骨, 骨量, 骨质

Abstract: Objective　To investigate the effect of CKIP-1 gene on the changes of trabecular bone of femur and mandibular at different time. Methods: 3-month- and 6-month-old mice with CKIP-1 knockout (CKIP-1-/-) were selected as the experimental group, wild type mice (WT) as the control group (n=5, same litter and gender, male). Femurs and mandibulars at 3 months and 6 months were harvested for micro-CT scanning. Quantitative analysis was performed to analyze the trabecular bone of the femur and the mandible. The effect of CKIP-1 on bone metabolism balance in adults with the increase of age was evaluated. Results: At 3 months, compared with the control group, the BV/TV of the femur and the mandible in the experimental group increased by 2.54% and 1.69%, and at 6 months increased by 3.77% and 2.82%（P<0.05）, respectively. During the period from 3 months to 6 months, BV/TV of the femur and mandible decreased by 4.42% and 1.1% (P<0.05) respectively. Conclusion: The femur is more sensitive than the mandible to the CKIP-1 gene regulation of bone mass, and it is more obvious with the time.

Key words: CKIP-1, Micro-CT, cancellous bone, bone mass, bone quality

中图分类号:

秦东泽胡奥郭威孝梁建飞蒋威许珊珊曹强张浚睿陆斌孔亮. CKIP-1对不同时间和部位松质骨影响的Micro-CT分析[J]. , 2017, 37(3): 198-202.

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