乏氧对IL-17调控牙周膜细胞RANKL及OPG表达的影响

›› 2017, Vol. 37 ›› Issue (5): 385-389.

• 基础研究 • 下一篇

乏氧对IL-17调控牙周膜细胞RANKL及OPG表达的影响

茅飞飞¹,李璐¹,王振婷²,王玉珍²,徐艳³

1. 南京医科大学口腔疾病研究江苏省重点实验室；南京医科大学附属口腔医院
2. 南京医科大学口腔疾病研究江苏省重点实验室
3. 南京医科大学附属口腔医院牙周科

收稿日期:2016-11-25 修回日期:2016-12-29 出版日期:2017-05-28 发布日期:2017-05-19
通讯作者: 茅飞飞 E-mail:feifei_mao@yeah.net
基金资助:
国家自然科学基金;江苏省高等学校大学生创新创业训练计划资助项目;江苏省高校自然科学研究面上项目

Effect of IL-17 on modulating the expression of RANKL and OPG in periodontal ligament cells under hypoxia

Received:2016-11-25 Revised:2016-12-29 Online:2017-05-28 Published:2017-05-19

摘要/Abstract

摘要： 目的探究不同乏氧条件下IL-17对人牙周膜成纤维细胞RANKL及OPG表达的影响。方法在常氧（氧体积分数20%）及不同乏氧条件下:轻度乏氧(氧体积分数10%)、中度乏氧（氧体积分数5%）、重度乏氧（氧体积分数2%) IL-17处理人牙周膜成纤维细胞24 h。通过CCK8比色法检测细胞增殖情况，实时定量RT-PCR和Western-blot方法检测细胞中骨吸收相关因子RANKL及骨保护因子OPG的表达水平。结果氧体积分数10%、5%时，人牙周膜成纤维细胞的增殖与时间呈正相关关系，氧体积分数2%时，细胞增殖受到明显抑制。乏氧培养后随氧体积分数的降低，人牙周膜成纤维细胞RANKL蛋白及mRNA的表达升高，OPG的蛋白及mRNA表达呈现先升后降的趋势。结论当氧体积分数在5%左右时最大程度上调RANKL/OPG比例，提示中度乏氧可促进IL-17调控人牙周膜成纤维细胞骨吸收相关因子的表达，并参与牙槽骨的改建。

关键词: 乏氧, 牙周膜成纤维细胞, 白介素17 , 低氧诱导因子, 骨吸收相关因子, 骨保护因子

Abstract: Objective To investigate the effect of IL-17 on modulating the expression of RANKL and OPG in human periodontal ligament cells（hPDLCs）under hypoxia. Methods Human PDLCs were incubated in the normoxia (normal oxygen tension) and different hypoxic atmospheres (oxygen volume fraction 10%, 5%, 2%) for 24 hours. After that, cell proliferation assay was determined using CCK-8 technique. The expression levels of RANKL and OPG were determined by western blotting and real-time PCR. Results Human PDLCs proliferation increased in the normoxia and the hypoxic atmosphere of 10% O and 5% O and had a positive correlation with time in a time-dependent manner while the hPDLCs proliferation was inhibited in the hypoxic atmosphere of 2% O. With the decrease of oxygen concentration under hypoxia, OPG mRNA and protein levels were up-regulated at first and then down-regulated meanwhile RANKL mRNA and protein levels were up-regulated after stimulated by hypoxia. Conclusions The relative RANKL/OPG expression ratios increase mostly in a hypoxic atmosphere of 5% O, suggesting that moderate hypoxia can modulate the alveolar remodeling via regulating the expressions of pro-osteoplastic molecules of hPDLCs through IL-17.

Key words: Hypoxia, periodontal ligament cells, IL-17, HIF-1α, RANKL, OPG

中图分类号:

R329.28

茅飞飞李璐王振婷王玉珍徐艳. 乏氧对IL-17调控牙周膜细胞RANKL及OPG表达的影响[J]. , 2017, 37(5): 385-389.

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乏氧对IL-17调控牙周膜细胞RANKL及OPG表达的影响

Effect of IL-17 on modulating the expression of RANKL and OPG in periodontal ligament cells under hypoxia

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