关键词: 新型3F-PAc树脂基质, Bis-GMA, TEGDMA, MTT, 细胞毒性

Abstract: Objective To evaluate the cytotoxicity of a new type of low polymerization shrinkage resin matrix material on L929 mouse fibroblast cells. Methods The three fluorine meta-position methyl phenyl acrylate 3F-PAc was synthesized by our experimental group, which is a low polymerization shrinkage resin matrix. Then it was compared with the traditional clinical commonly used resin matrix Bis-GMA, and the experiment group was divided into three groups, Bis-GMA, 3F-PAc 1 and 3F-PAc 0 group, among which 3F-PAc 0 group did not add the TEGＤＭA diluent agent. The immersed solution of the three groups of material was diluted to four grades 100%, 50%,25% and 12.5%; the methyl thiazolyl tetrazolium(MTT) test was used to detect the relative cell proliferation rate of the mouse fibroblast cell line L929 co-cultured with the different solutions for 1, 3, 5 days. Compared with the negative control group, the cell toxicity grade was evaluated. Results All the cytotoxicity of the experimental group extracts was grade 0~1,except that the 100% extract of Bis-GMA and 3F-PAc 1 group were 2. The absorbance value (D) of 3F-PAc 0 groups was significantly higher than that of 3F-PAc 1 group and Bis-GMA group (P<0.05), and the difference was statistically significant. However, the difference between 3F-PAc 1 group and Bis-GMA group was not significant (P>0.05). Conclusions The new 3F-PAc resin matrix material has no cytotoxicity but has comparatively good biosecurity. The TEGＤＭA diluent agent may be a part reason of the cytotoxicity of the resin material.

Key words: New 3F-PAc resin matrix, Bis-GMA, TEGDMA, MTT assay, Cytotoxicity