牙龈卟啉单胞菌脂多糖对人脐静脉内皮细胞MMP-9表达影响的研究

›› 2017, Vol. 37 ›› Issue (5): 394-397.

牙龈卟啉单胞菌脂多糖对人脐静脉内皮细胞MMP-9表达影响的研究

刘岩¹,李维善¹,苏红影²,邵伟然³,王泽⁴

1. 佳木斯大学附属口腔医院
2. 黑龙江省佳木斯市佳木斯大学附属第二医院口腔医院
3. 佳木斯大学
4. 佳木斯大学口腔医学院

收稿日期:2016-09-01 修回日期:2016-10-31 出版日期:2017-05-28 发布日期:2017-05-19
通讯作者: 李维善 E-mail:weishanli666@126.com
基金资助:
佳木斯大学研究生科技创新项目

Studies on the influence of P.g-LPS on the expression of MMP-9 in HUVECS

Received:2016-09-01 Revised:2016-10-31 Online:2017-05-28 Published:2017-05-19

摘要/Abstract

摘要： 目的采用牙龈卟啉单胞菌脂多糖（Porphysomanas gingivalis lipopolysaccharide, P.gingivalis-LPS)感染人脐静脉内皮细胞（HUVECs），观察感染的内皮细胞中金属基质蛋白酶-9（MMP-9）的变化,探讨P.gingivalis与动脉粥样硬化(atherosclerosis，AS)的关系。方法体外培养HUVECs，将不同浓度的P.gingivalis-LPS分别加入到单层融合后的HUVECs中, 共同培养6、18、24 h,采用酶联免疫吸附实验（enzyme linked immunosorbent assay,ELISA）检测HUVECs表达MMP-9的量。MTT法检测P.gingivalis-LPS对HUVECs增殖的影响。结果本研究条件下,应用不同浓度的P.gingivalis-LPS刺激HUVECs 6、18、24 h后，细胞表达MMP-9的量升高(P<0.05)。经P.gingivalis-LPS处理后，HUVECs增殖活力较对照组明显下降(P<0.01)。结论 P.gingivalis-LPS能够上调HUVECs表达MMP-9的水平，进而加速AS的发生与发展。

关键词: 牙龈卟啉单胞菌脂多糖, 人脐静脉内皮细胞, 基质金属蛋白酶-9, 动脉粥样硬化

Abstract: Objective To use the Porphyromonas gingivalis ipopolysaccharide(P.gingivalis-LPS) to infect human u-mbilical vein endothelial cells (HUVECs), then to observe the change of matrix metalloproteinase-9(MMP-9) in infected endothelial and to discuss the relationship between P.gingivalis and atherosclerosis (atherosclerosis,AS) at the same time. Methods First HUVECs were cultured in vitro, and different concentrations of P.gingivalis-LPS were put into the cultivated cell solution after fusion to monolayers. Then enzyme linked immunosorbent assay (enzyme linked immunosorbent assay, ELISA) was technically used for detecting the expression of MMP-9 in HUVECs as co-cultured for 6,18,24 hours respectively. The influence of P.gingivalis-LPS on HUVECs proliferation was detected by MTT assay. Results Under the environments of our experiment, the expression levels of MMP-9 increased (P<0.05) after the stimulation on HUVECs with different concentrations of P.gingivalis-LPS for 6,18, 24 hours. Compared with control group, after the treatment of P.gingivalis-LPS, HUVECs showed obviously decreased proliferation viability(P<0.01). Conclusion P.gingivalis-LPS can increase the expression level of MMP-9 in HUVECs, and then accelerate the occurrence and development of AS.

Key words: P.gingivalis-LPS, human umbilical vein endothelial cell, MMP9, atherosclerosis

中图分类号:

R780.2

刘岩李维善苏红影邵伟然王泽. 牙龈卟啉单胞菌脂多糖对人脐静脉内皮细胞MMP-9表达影响的研究[J]. , 2017, 37(5): 394-397.

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