纳米羟基磷灰石在骨重建中的效应和机理

摘要/Abstract

摘要： 由于与骨基质矿物质成份的相似性，纳米羟基磷灰石（nano- hydroxyapatite, nano-HAP）及其复合物在生物医疗再生领域引起了广泛关注。然而，我们对于nano-HAP在骨再生过程中的作用及机制尚未明确。此外，对于nano-HAP对机体是否有毒性仍有争议。本文就其在骨缺损修复方面的应用，促进骨再生的机制和生物安全性作一综述。

关键词: 纳米羟基磷灰石, 骨再生, 机制, 生物安全性

Abstract: Due to chemical similarity of nano-hydroxyapatite (nano-HAP) to inorganic component of bone matrix, nano-HAP and its composites have attracted particular attention in the field of regenerative medicine. However, our understanding of the effect and mechanism of nano-HAP on bone regeneration is still fragmentary. In addition, very little is known about the interaction of nano-hydroxyapatite with the body with regards to its toxicity. In this article, the application, mechanism and biological safety of nano-HAP in bone repair will be reviewed.

Key words: nanophase hydroxyapatite, bone regeneration, mechanism, biological safety

中图分类号:

R783.1

周凯黄菲张娟王琛. 纳米羟基磷灰石在骨重建中的效应和机理[J]. , 2017, 37(5): 453-456.

参考文献

[1]Ratnayake J T B,Mucalo M,Dias G J.Substituted hydroxyapatites for bone regeneration: A review of current trends[J].J Biomed Mater Res B Appl Biomater,2016,100B(6):1-15
[2]Ohare P,Meenan B J,Burke G A,et al.Biological responses to hydroxyapatite surfaces deposited via a co-incident microblasting technique[J].Biomaterials,2010,31(3):515-522
[3]Shi Z,Huang X,Cai Y,et al.Size effect of hydroxyapatite nanoparticles on proliferation and apoptosis of osteoblast-like cells[J].ActaBiomaterialia,2009,5(1):338-345
[4]Wang Z X,Yi H,Wei H,et al.Effects of Hydroxyapatite Nanoparticles on Apoptosis and Invasion of Human Renal Cell Carcinoma 786-0 Cells[J].Chemical Research in Chinese Universities,2011,27(1):94-98
[5]Do T N,Lee W H,Loo C Y,et al.Hydroxyapatite nanoparticles as vectors for gene delivery[J].Therapeutic Delivery,2012,3(5):623-632
[6]Dorozhkin S V.Nanosized and nanocrystalline calcium orthophosphates[J].ActaBiomaterialia,2010,6(3):715-734
[7]Giannoudis P V,Dinopoulos H,Tsiridis E.Bone substitutes: An update[J].Injury-international Journal of the Care of the Injured,2005,36 Suppl 3(3):20-27
[8]Nandi S K,Roy S,Mukherjee P,et al.Orthopaedic applications of bone graft & graft substitutes: a review[J].Indian J Med Res,2010,132(7):15-30
[9]Akram M,Ahmed R,Shakir I,et al.Extracting hydroxyapatite and its precursors from natural resources[J].J Mater Sci Mater Med,2014,49(4):1461-1475
[10]Sun W,Chu C,Wang J,et al.Comparison of periodontal ligament cells responses to dense and nanophase hydroxyapatite[J].J Mater Sci Mater Med,2007,18(5):677-683
[11]Lim P N,Lei C,Thian E S.Development of nanosized silver-substituted apatite for biomedical applications: A review[J].Nanomedicine Nanotechnology Biology & Medicine,2015,11(6):1331-1344
[12]Bral A,Mommaerts M Y.In vivo biofunctionalization of titanium patient-specific implants with nano hydroxyapatite and other nano calcium phosphate coatings: A systematic review[J].J Craniomaxillofac Surg,2016,44(4):400-412
[13]Abd E H,Helmy Y,El-Kholy B,et al.In vivo animal histomorphometric study for evaluating biocompatibility and osteointegration of nano-hydroxyapatite as biomaterials in t4 engineering[J].J Egypt Natl Canc Inst,2010,22(4):241-250
[14]Zhu X,Eibl O,Scheideler L,et al.Characterization of nano hydroxyapatite/collagen surfaces and cellular behaviors[J].Journal of Biomedical Materials Research Part A,2006,79(1):114-127
[15]Bhattacharyya S,Kumbar S G,Khan Y M,et al.Biodegradable polyphosphazene-nanohydroxyapatite composite nanofibers: scaffolds for bone t1 engineering[J].J Biomed Nanotechnol,2009,5(1):69-75
[16]Ribeiro N,Sousa S R,van Blitterswijk C A,et al.A biocomposite of collagen nanofibers and nanohydroxyapatite for bone regeneration[J].Biofabrication,2014,6(6):035015-035015
[17]Ning L,Malmstrom H,Ren Y F.Porous collagen-hydroxyapatite scaffolds with mesenchymal stem cells for bone regeneration[J].J Oral Implantol,2013,41(1):45-49
[18]Lunov O,SyrovetsT,LoosC,et al.Differential uptake of functionalized polystyrene nanoparticles by human macrophages and a monocytic cell line[J].ACS nano,2011,5(3):1657-1669
[19]Marsh M,Mcmahon H T.The Structural Era of Endocytosis[J].Science,1999,285(5425):215-220
[20]Wang C,Liu D,Zhang C,et al.Defect-Related Luminescent Hydroxyapatite-Enhanced Osteogenic Differentiation of Bone Mesenchymal Stem Cells Via an ATP-Induced cAMP/PKA Pathway[J].ACS Appl Mater Interfaces,2016,8:11262-11271
[21]Ha S W,Jang H L,Nam K T,et al.Nano-hydroxyapatite modulates osteoblast lineage commitment by stimulation of DNA methylation and regulation of gene expression[J].Biomaterials,2015,65:32-42
[22]Zhao Y,Zhang Y,Ning F,et al.Synthesis and cellular biocompatibility of two kinds of HAP with different nanocrystal morphology[J].J Biomed Mater Res B Appl Biomater,2007,83B(1):121-126
[23]Yang W,Han W,He W,et al.Surface topography of hydroxyapatite promotes osteogenic differentiation of human bone marrow mesenchymal stem cells[J].Mater Sci Eng C Mater Biol Appl,2016,60:45-53
[24]Xia L,Lin K,Jiang X,et al.Enhanced osteogenesis through nano-structured surface design of macroporous hydroxyapatite bioceramic scaffolds via activation of ERK and p38 MAPK signaling pathways[J].J.mater.chem.b,2013,1(40):5403-5416
[25]Liu H,Peng H,Wu Y,et al.The promotion of bone regeneration by nanofibrous hydroxyapatite/chitosan scaffolds by effects on integrin-BMP/Smad signaling pathway in BMSCs[J].Biomaterials,2013,34(18):4404-4417
[26]Mao L,Liu J,Zhao J,et al.Effect of micro-nano-hybrid structured hydroxyapatite bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via Wnt signaling pathway[J].Int J Nanomedicine,2015,10(1):7031-7044
[27]Lim P N,Lei C,Thian E S.Development of nanosized silver-substituted apatite for biomedical applications: A review[J].Nanomedicine Nanotechnology Biology & Medicine,2015,11(6):1331-1344
[28]Tan Y Y,Zhang D,Li Y X,et al.Relative biological evaluation of hydroxyapatite nanocrystals surface-grafted with L-lactic acid oligomer/poly(lactide-co-glycolde) cultured with bone marrow mesenchymal stem cells[J].Journal of Clinical Rehabilitative T25 Engineering Research,2011,15(25):4619-4622
[29]Zhang B P,Tang S H,Zhang L,et al.Treatment of osteomyelitis in rabbits with plastic nanohydroxyapatite/poly (3-hydroxybutyrate-hydroxyvalerate)-polyethylene glycol drug delivery system[J].Journal of Clinical Rehabilitative T21 Engineering Research,2011,15(21):3871-3876
[30]Jeanne S,Tricot-Doleux S,Pellen-Mussi P,et al.Effect of hydroxyapatite and beta-tricalcium phosphate nanoparticles on promonocytic U937 cells[J].J Biomed Nanotechnol,2010,6(2):158-165
[31]Sha J M,Yan Z Y,Cheng G C,et al.In-vitro seeding of human umbilical cord vein endothelial cells on hydroxyapatite for mechanical heart valve applications[J].J Heart Valve Dis,2010,19(4):506-512
[32]Hong L,Li,Wang M,et al.Preparation and Biological Safety Evaluation of Porous n-HA/PA66 Composite[J].Journal of Biomedical Engineering,2008,25(5):1126-1129
[33]Sotome S,Ae K,Okawa A,et al.Efficacy and safety of porous hydroxyapatite/type 1 collagen composite implantation for bone regeneration: A randomized controlled study[J].J Orthop Sci,2016,21(3):373-380
[34]Napierska D,Thomassen L C J,Rabolli V,et al.Size-Dependent Cytotoxicity of Monodisperse Silica Nanoparticles in Human Endothelial Cells[J].Small,2009,5(7):846-853
[35]Oberd?rster G,Sharp Z,Atudorei V,et al.Extrapulmonary translocation of ultrafine carbon particles following whole-body inhalation exposure of rats[J].Journal of Toxicology & Environmental Health Part A,2002,65(20):1531-1543
[36]Liu L,Xiao Z,Xiao Y,et al.Combination of high-intensity focused ultrasound irradiation and hydroxyapatite nanoparticle injection to injure normal goat liver t4 in vivo without costal bone incision[J].Genetics & Molecular Research Gmr,2014,13(4):8301-8308
[37]Ding T,Xue Y,Lu H,et al.Effect of particle size of hydroxyapatite nanoparticles on its biocompatibility[J].IEEE Trans Nanobioscience,2012,11(4):336-340
[38]Wang L,Zhou G,Liu H,et al.Nano-hydroxyapatite particles induce apoptosis on MC3T3-E1 cells and t9 cells in SD rats[J].Nanoscale,2012,4(9):2894-2899
[39]Wennerberg A,Jimbo R,Allard S,et al.In vivo stability of hydroxyapatite nanoparticles coated on titanium implant surfaces[J].Int J Oral Maxillofac Implants,2011,26(6):1161-1166