Objective　 To investigate the attitude of five-year undergraduate interns towards rubber dam use and to feedback the problems that exist in the clinical teaching and analyze the causes and countermeasures.Methods　A questionnaire-based survey was conducted to the 102 five-year dental school students (29 male and 73 female) and it consisted of relevant questions to ascertain the attitudes, opinions and practices concerning rubber dam use, including usage rates, reasons for usage and non usage, difficulties during usage, technical proficiency, conception problems, patient acceptance and compliance. Data were analyzed using SPSS 21.0 software. Excel form of Microsoft 2007 was used to make a double entry for checking. And chi-square test was applied to check the difference among different groups(α= 0.05). Results　The results showed that the rubber dam using rate was 65.68%. The reasons for non usage of rubber dam were difficulty in placement, being time-consuming, lack of theoretical basis, insufficient experience, clinical habits and refusal of use. In this study, 64.18% of the students could finish setting rubber dam within 5 min. Whereas 100% of students indicated that they prefer to using rubber dam after graduation and setting technique should be mastered for all students.Conclusions　The extensive use of rubber dam technique is still on the way. Dental schools should take responsibility of training students how to use rubber dam to promote the using rate. There is some reluctance about future integration of this tool in routine practice. Therefore, greater emphasis should be put on rubber dam in clinical treatment and improving the level of clinical treatment.