不同咀嚼压力对幼兔髁突软骨成骨的影响研究

摘要/Abstract

摘要： 目的：分析生长发育期不同咀嚼模式下兔髁突软骨成骨形态学的差异，研究咀嚼压力对幼兔髁突生长发育的影响和作用机制。方法：选取15天龄的未断奶幼兔16只，随机分为硬食组和软食组各8只，硬食组提供颗粒饲料，软食组提供由同种颗粒饲料碾磨而成的粉末状饲料。幼兔28天龄断奶，分别于28天龄、33天龄肌肉注射四环素、钙黄绿素，38天龄处死幼兔。制作硬组织切片，荧光显微镜观察，独立t检验分析比较两组髁突软骨成骨厚度，骨形态学参数。结果：硬食组的软骨成骨厚度、骨小梁相对体积、骨小梁数量、骨小梁厚度均大于软食组（P<0.05），硬食组骨小梁分离度小于软食组（P<0.05）。相同时间硬食组骨形成沉积率[（3.25±0.02）μm / d ]大于软食组[（2.37±0.02）μm / d ]。结论：生长期软食组幼兔的髁突软骨成骨能力较硬食组弱，咀嚼负荷力是影响髁突软骨成骨生长的重要因素。

关键词: 髁突, 软骨成骨, 咀嚼力

Abstract: Objective To analyze the histomorphometry differences of condylar cartilage osteogenesis under altered masticatory loading, and to study the mechanism of masticatory loading on condylar development in growing rabbits. Methods sixteen 15-day-old rabbits were randomly divided into two groups and fed different kinds of food, such as solid diet (n=8) and soft diet (n=8). ALL the growing rabbits were weaned at 28-day-old. Tetracycline (50mg/kg), calcein (20mg/kg) and were given intramuscularly on the 28-day-old and 33-day-old. The rabbits were sacrificed on the 38-day-old. The specimens of condyles were embedded in methylmethacrylate. Undecalcified hard tissue slicings (20mm) were then made and observed with a fluorescence microscope. We measured the cartilage osteogenesis thickness and the histomorphometry parameters of the condyle in two groups and did statistical analysis. Results The statistical analysis showed that there was significant difference in the cartilage osteogenesis thickness, percent trabecular area, trabecular number, trabecular thickness and trabecular separation in the two groups (P<0.05). The solid diet groups exceeded those of the soft diet groups except trabecular separation. The mineral appositeion rate of solid diet groups [ (3.25±0.02)μm / d ] was higher than soft diet groups [ (2.37±0.02)μm / d ]. Conclusion The condylar cartilage osteogenesis in growing rabbits soft diet groups was weaker than hard diet groups. Mastication is an important factor which affecting the growth of the condylar cartilage osteogenesis.

Key words: Condyle, Cartilage osteogenesis, Mastication

中图分类号:

R782.6

牟婷琛冯剑颖阎帆杨艺. 不同咀嚼压力对幼兔髁突软骨成骨的影响研究[J]. , 2018, 38(10): 868-871.

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