白介素-17对破骨前体细胞RAW264.7增殖分化的影响

›› 2018, Vol. 38 ›› Issue (10): 872-875.

白介素-17对破骨前体细胞RAW264.7增殖分化的影响

郭超¹,张勤²,陈晓涛³,王健³

1. 新疆医科大学
2. 新疆医科大学附属中医医院口腔科
3. 新疆维吾尔自治区人民医院口腔科

收稿日期:2018-04-20 修回日期:2018-05-18 出版日期:2018-10-28 发布日期:2018-10-30
通讯作者: 郭超 E-mail:2217072235@qq.com
基金资助:
牙周致病菌信号分子AI-2诱导Th亚群细胞在慢性牙周炎微环境表达的机制研究

Influence of IL-17 on RAW264.7 cells proliferation and differentiation

Received:2018-04-20 Revised:2018-05-18 Online:2018-10-28 Published:2018-10-30

摘要/Abstract

摘要： 探讨白细胞介素-17对破骨前体细胞RAW264.7增殖分化功能的影响。方法：采用RANKL和M-CSF刺激破骨前体细胞系RAW264.7培养24小时后，将IL-17干预细胞培养。采用CCK-8试剂盒，及实时荧光定量PCR，分别评价破骨前体细胞增殖和特征性基因的表达差异。结果：IL-17A促进小鼠RAW264.7细胞增殖，且呈时间、浓度依赖性；IL-17A浓度在50mg/ml时，特征性基因CathK，RANK和NAFTC1 mRNA表达升高，差异均有统计学意义（P<0.05）。结论：IL-17A促进RAW264.7细胞增殖，促进破骨前体细胞分化。

关键词: 白细胞介素-17, RAW264.7细胞, 增殖, 分化

Abstract: To explore the effects of IL-17A to promote proliferation and differentiation of preosteoclasts RAW264.7 cells. Methods: RAW264.7 cells were cultured by RANKL and M-CSF at 24h after treated with IL-17A. CCK-8 kits and RT-PCR were used to evaluate the effects of proliferation and differentiation. Results: IL-17A could promote proliferation of RAW264.7 cells, the proliferation effects of IL-17A had a dose-effect relationship and was in a time-dependent manner; At the concentration of 50ng/ml of IL-17A, the activity of genes including CathK，RANK and NAFTC1 mRNA increased. Conclusion: It is demonstrated that IL-17A mainly promotes RAW264.7 cells proliferation, and promotes RAW264.7 cells differentiation.

Key words: IL-17A, RAW264.7, proliferation, differentiation

中图分类号:

R349.5

郭超张勤陈晓涛王健. 白介素-17对破骨前体细胞RAW264.7增殖分化的影响[J]. , 2018, 38(10): 872-875.

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