麻醉对肥胖复合牙周炎模型副作用的研究

›› 2018, Vol. 38 ›› Issue (10): 885-889.

麻醉对肥胖复合牙周炎模型副作用的研究

余挺¹,赵莉²,³,章锦才²,⁴,轩东英⁵

1. 广州医科大学附属口腔医院
2.
3. 中山大学光华口腔医学院?附属口腔医院，广东省口腔医学重点实验室
4. 中国科学院大学·存济医学院
5. 中国科学院大学存济医学院·附属杭州口腔医院

收稿日期:2018-04-02 修回日期:2018-05-15 出版日期:2018-10-28 发布日期:2018-10-30
通讯作者: 轩东英 E-mail:xuanxuan187@126.com
基金资助:
国家自然科学基金;国家自然科学基金;国家自然科学基金

Anesthetic adverse effects in diet-induced obese mice treated for ligation-induced periodontitis

Received:2018-04-02 Revised:2018-05-15 Online:2018-10-28 Published:2018-10-30
Contact: Dong-ying XUAN E-mail:xuanxuan187@126.com

摘要/Abstract

摘要： 目的：探索麻醉副作用相关内脏病变在饮食诱导型肥胖（DIO）联合结扎诱导牙周炎（LIP）模型中的分布情况。方法：建立DIO与LIP（牙周结扎5或10 d）联合小鼠模型（n = 120），LIP于4% 水合氯醛腹腔麻醉下完成，大体解剖评估腹腔内脏病变。结果：麻醉副作用相关内脏病变累及14.8%（17/115）小鼠，涉及附睾、内脏脂肪、肾、脾、肝，且发生于同一侧。DIO小鼠的内脏病变、粘连的占比分别高于体重正常组1倍（18.1% vs. 9.3%，P = 0.31）和2倍（8.3% vs. 2.3%，P = 0.37），尽管无统计学意义。10 d组的内脏病变、粘连的占比分别高于5 d组1倍（19.0% vs. 10.5%，P = 0.31）和4倍（10.3% vs. 1.8%，P = 0.12），尽管无统计学意义。结论：建立肥胖与牙周炎的联合模型时，应慎重评估AAEs对两者相关性的混杂影响。

关键词: 麻醉, 动物模型, 肥胖, 牙周炎, 副作用

Abstract: Objective To explore anesthetic adverse effects (AAEs) related visceral organ lesions in combined diet-induced obese (DIO) and ligation-induced periodontitis (LIP) models. Methods Combined DIO (16-week on diet) and LIP (5- or 10-day ligation) mouse (C57 BL/6J, male) models were studied (n = 120). Following DIO period, LIP was established under anesthesia with 4% w/v chloral hydrate (i.p.), with sham-ligation as the control. Abdominal organ lesions related to AAEs were assessed by general necropsy. Results AAEs-related organ lesions were found in 14.8% (17/115) of the mice. The involved organs included epididymis, visceral adipose tissue, kidney, spleen and liver, which were all lesioned on the same side. Despite no significance, the proportions of organ lesions and adhesion were 1 (18.1% vs. 9.3%, P = 0.31) and 2 (8.3% vs. 2.3%, P = 0.37) times higher, respectively, in DIO mice than those in normal-weight controls and were 1 (19.0% vs. 10.5%, P = 0.31) and 4 (10.3% vs. 1.8%, P = 0.12) times higher, respectively, after 10 days of ligation than those after 5 days of ligation. Conclusions Potential confounding effects of AAEs should be critically assessed when studying the correlation between obesity and periodontitis.

Key words: Anesthesiology, Models, animal, Obesity, Periodontal diseases, Side effects

余挺赵莉章锦才轩东英. 麻醉对肥胖复合牙周炎模型副作用的研究[J]. , 2018, 38(10): 885-889.

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