富血小板纤维蛋白联合天然煅烧骨对颅骨缺损修复作用研究

›› 2018, Vol. 38 ›› Issue (11): 970-975.

富血小板纤维蛋白联合天然煅烧骨对颅骨缺损修复作用研究

陈铁楼¹,张新海²,许兵³,徐东升⁴,王世峰⁵,秦卫民⁶,刘进⁷,王海彗⁸,王晓熙⁹,任正波⁸

1. 海军军医大学第一附属医院全军口腔疾病诊治中心牙周科
2. 海军军医大学解放军411医院全军口腔疾病诊治中心牙周科
3. 上海同济大学
4. 上海宝山区中西医结合医院
5. 解放军海军医学研究所
6. 海军军医大学第一附属医院全军口腔中心牙周科,
7. 上海解放军411医院口腔牙周科
8. 海军军医大学第一附属医院全军口腔中心牙周科
9. 海军军医大学第一附属医院病理科

收稿日期:2018-04-23 修回日期:2018-06-10 出版日期:2018-11-28 发布日期:2018-11-30
通讯作者: 陈铁楼 E-mail:xbctl@sh163.net
基金资助:
上海市卫计委项目;总后面上项目

Effects of combination platelet rich fibrin with calcined natural bovine bone on repairing rabbit skull defect

Received:2018-04-23 Revised:2018-06-10 Online:2018-11-28 Published:2018-11-30
Contact: Tie-Lou CHEN E-mail:xbctl@sh163.net

摘要/Abstract

摘要： ［摘要］目的探讨富血小板纤维蛋白（platelet rich fibrin,PRF）和天然煅烧骨（calcined natural bovine bone，CBB）对颅骨缺损修复疗效，为骨缺损修复提供依据。方法选18只成年兔在颅骨左右侧分别制作10 mm直径大小圆形骨缺损，分为实验1组（植入CBB）、实验2组（植入PRF和CBB）和对照组（不植骨）。在术后4周、12周、20周取颅骨标本，分别行甲苯胺蓝染色、四环素荧光染色、影像学CT测定各组骨缺损区新骨形成面积、成骨速度、移植物吸收和骨密度值。结果在术后4周、12周和20周时实验1组和实验2组新骨形成速度、成骨面积、成骨钙化程度及骨密度值均大于对照组；实验2组成骨面积和骨密度大于实验1组（P<0.05），中央网孔内成骨明显；对照组在12周和20周新骨形成明显减少。结论 CBB对颅骨缺损修复有明显疗效，PRF可促进CBB对骨缺损修复作用，可能与PRF的骨诱导作用有关。

关键词: 天然煅烧骨, 富血小板纤维蛋白, 颅骨缺损, 骨移植, 疗效

Abstract: Objective　To investigate the effects of combination platelet-rich fibrin (PRF) with calcined natural bovine bone(CBB) on repairing rabbit skull defect to provide evidence for treating bone defect. Methods: Eighteen adult rabbits were selected to make 10mm diameter circular bone defects in the left and right sides of the skull respectively. Bone defects were divided into three groups: experimental group 1 (CBB implantation), experimental group 2 (implantation of PRF and CBB) and control group (no bone graft). The skull specimens were collected at the 4th, 12th and 20th weeks after operation, respectively. Toluidine blue staining, tetracycline fluorescence staining and CT imaging were used to determine the osteogenic area, osteogenic velocity, graft absorption and bone mineral density. Results: 4, 12 and 20 weeks after operation, the rate of new bone formation, the degree of new bone calcification, the osteogenic area and the bone mineral density of experimental group 1 and experimental group 2 were all higher than those of control group. The bone area and bone density in experiment group 2 were larger than those in group 1 (P < 0.05), and the osteogenesis in central reticulum was obvious. In the control group, the formation of new bone obviously decreased at the 12th and 20th weeks. Conclusion: CBB has strong therapeutic effects on skull defect. PRF can improve the repair effects of bone defect by CBB, which may be related to the bone induction of PRF.

Key words: platelet-rich fibrin, calcined natural bovine bone, skull defect, curative effect, bone grafts.

中图分类号:

R782.4

陈铁楼张新海许兵徐东升王世峰秦卫民刘进王海彗王晓熙任正波. 富血小板纤维蛋白联合天然煅烧骨对颅骨缺损修复作用研究[J]. , 2018, 38(11): 970-975.

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