高通量测序方法分析实验室培养对人体口腔唾液菌群的影响

›› 2018, Vol. 38 ›› Issue (12): 1062-1067.

高通量测序方法分析实验室培养对人体口腔唾液菌群的影响

石雨婷¹,夏文君¹,邹岩¹,漆正楠²,段胜仲³,唐子圣¹

1. 上海交通大学医学院附属第九人民医院
2. 上海市口腔病防治院
3. 上海市口腔医学重点实验室/上海市口腔医学研究所，国家口腔疾病临床研究中心

收稿日期:2018-05-17 修回日期:2018-06-07 出版日期:2018-12-28 发布日期:2018-12-17
通讯作者: 唐子圣 E-mail:sweetyshi49@gmail.com
基金资助:
国家重点研发计划项目;中国科学院重大科技基础设施开放研究项目

Effect of culture in vitro on human salivary microflora by high throughput sequencing

Received:2018-05-17 Revised:2018-06-07 Online:2018-12-28 Published:2018-12-17

摘要/Abstract

摘要： 目的　研究实验室培养对口腔唾液菌群结构及其多样性的影响。方法　本实验选取6个健康且符合纳入排除标准的志愿者，采集口腔唾液菌群样本并进行实验室培养。基于Illumina Hiseq测序平台，我们利用16S rDNA高通量测序技术，研究实验室培养对人体口腔唾液细菌的影响，分析了培养前后的菌群物种丰度和菌群结构差异。结果　高通量测序一共得到618个OTUs，测序分析显示人体口腔唾液菌群在实验室培养前后，菌群生物多样性的差异无统计学意义（P＞0.05），菌群构成的差异无统计学意义（P＞0.05）。OTUs Venn图显示实验室培养前后，人体口腔唾液菌群拥有大约78.4%相同物种。结论　16S rDNA高通量测序分析显示，人体口腔唾液菌群在实验室培养前后具有相似性。

关键词: 16S rRNA, 口腔微生态, 唾液, 高通量测序

Abstract: Objective To study the effect of culture in vitro on oral microfloral structure and biodiversity. Methods Six healthy and eligible volunteers were selected. Oral bacterial samples were collected and cultured in vitro. Based on Illumina Hiseq sequencing platform, 16S rDNA high throughput sequencing technology was used to study the effect of culture in vitro on human oral microflora, and then the structure and relative abundance of microflora before and after culture were analyzed. Results There were a total of 618 OTUs in this sequencing. Alpha diversity showed that the difference of biodiversity had no statistical significance before and after culture in vitro. Beta diversity showed that no significant difference was observed in bacterial structure. According to the OTUs Venn diagram, there were about 78.4% identical OTUs before and after culture in human oral salivary flora. Conclusion 16S rDNA high throughput sequencing analysis shows that human oral salivary flora has similarity before and after culture in vitro.

Key words: 16S rRNA, oral bacteria flora, saliva, high throughput sequencing

中图分类号:

R466.5

石雨婷夏文君邹岩漆正楠段胜仲唐子圣. 高通量测序方法分析实验室培养对人体口腔唾液菌群的影响[J]. , 2018, 38(12): 1062-1067.

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