关键词: 光固化壳聚糖, 胶原, 支架, 位点保存

Abstract: Objective To analyze the application of the novel chitosangelatin 3D scaffold in socket preservation therapy. Methods The novel chitosangelatin 3D scaffold was composed by dimethyl sulfoxide (DMSO)/UV crosslink. Then the scaffold was compounded with rhBMP2. Eight beagle dogs, sixteen sockets after teethextraction in the left and right mandibles were selected. The sockets were divide into control group, empty scaffold group (S0 group), scaffold+ heparin group (SH group), scaffold+BMP2 group (SB group) by random. The scaffolds were implanted in each group following the group division. The animals were put into death 90 days after the surgery. The heights and widths of each socket’s alveolar bone were measured. Then the socket’s alveolar bones were analyzed by micro CT and histology. Results The alveolar bone width and height loss in S0, SH, SB groups were less than those of control group (P<0.05). By comparison between groups, SB group and SH group were less than S0 (P<0.05). Micro CT 3D reconstruction showed that S0 group, SH group and SB group were better in retaining the original alveolar ridge width and shape. The control group obviously had narrow alveolar ridge, and there were some local absorptions; histologically, in SB group no bone was absorbed, a mature bone trabeculae formed and bone calcification degree was higher than other groups. Conclusion The new threedimensional scaffold can achieve good bone formation in animal models, which can maintain the height and width of alveolar ridge after tooth extraction.

Key words: photocured chitosan, gelatin, scaffold, alveolar ridge preservation therapy