羟基喜树碱纳米胶束对舌癌RSCC-1细胞体外抑制实验

摘要/Abstract

摘要： 目的探讨HCPT/PEGPLA（羟基喜树碱聚乙二醇聚乳酸）纳米胶束对舌癌RSCC1细胞体外抑制作用。方法兔舌癌RSCC1细胞复苏和培养，传代至对数生长期进行实验。经与PBS、不同浓度的HCPT（羟基喜树碱）和HCPT/PEGPLA共同培养不同时间后， MTT法检测体外细胞毒作用，计算细胞生长抑制率。结果 MTT检测结果显示HCPT市售剂型组呈现明显细胞毒作用：生长抑制率随药物浓度、作用时间增加而升高；HCPT/PEGPLA纳米胶束剂型组48 h时生长抑制率低于HCPT市售剂型组(P<0.05)，至96 h时则与HCPT市售剂型组差别己无统计学差异(P>O.05)。结论 ①HCPT/PEGPLA纳米胶束对RSCC1细胞具有直接抑制作用

关键词: 羟基喜树碱纳米胶束, 舌癌RSCC-1细胞, 细胞毒性

Abstract: Objective To investigate the inhibitory effect of HCPT/PEGPLA nano micelles on RSCC1 cells in vitro. Methods Rabbit tongue cancer cells RSCC1 were recovered, cultured and passed to the logarithmic growth phase experiment. After cocultured for different times with PBS group, different concentrations of HCPT group and HCPT/PEGPLA group, MTT method was used to detect cytotoxic effect in vitro, and cell growth inhibition rate was calculated. Results MTT assay showed that HCPT common dosage group showed significant cytotoxicity and the growth inhibition rate increased with the increase of drug concentration and action time. The growth inhibition rate of HCPT/PEGPLA nano micelles dosage group at 48h was less than HCPT common dosage group (P<0.05), and at 96 h,there was no statistical difference compared with HCPT common dosage (P>0.05). Conclusions ① HCPT/PEGPLA nano micelle has a direct inhibitory effect on the RSCC 1 cells, showing time and dosedependent properties. ② HCPT/PEGPLA nano micelle has sustained release.

Key words: hydroxyeamptotheeine nano micelles, rabbit tougue squamous carcinoma cell RSCC-1 , cytotoxicity

中图分类号:

R739.86

马萍李琦金武龙. 羟基喜树碱纳米胶束对舌癌RSCC-1细胞体外抑制实验[J]. , 2018, 38(2): 120-123.

参考文献

[1]Lee SW，Yun MH，Jeong SW，et al.Development of docetaxel-loaded intravenous formulation，Nanoxel- PM ? using polymer-based delivery system[J].J Control Release, 2011, 155(2):262-271
[2]Mu CF，Balakrishnan P，Cui FD，et al.The effects of mixed MPEG- PLApluronic copolymer micelles on the bioavailability and multidrug resistance of docetaxel[J].Biomaterials, 2010, 31(8):2371-2379
[3]Tran PH, Tran TT, Vo TV, et al.Novel multifunctional biocompatible gelatin-oleic acid conjugate : self-assembled nanoparticles for drug delivery[J].J Biomed Nanotechnol, 2013, 9(8):1416-1431
[4] Augustin MA, Sanguansri L, Lockett T.Nano- and micro-encapsulated systems for enhancing the delivery of resveratrol [J]. Ann N Y Acad Sci, 2013, 1290:107-112.
[5]Snehalatha M, Kolachina V, Saha RN, et al.Enhanced tumor uptake,biodistribution and pharmacokinetics of etoposide loaded nanoparticles in Dalton's lymphoma tumor bearing mice[J].J Pharm Bioallied Sci, 2013, 5(4):290-297
[6]Yang T, Sheng HH, Feng NP, et al.Preparation of andrographolide-loaded solid lipid nanoparticles and their in vitro and in vivo evaluations: characteristics,release,absorption,transports,pharmacokinetics,and antihyperlipidemic activity[J].J Pharm Sci, 2013, 102(12):4414-4425
[7]Wall ME, Wani MC, Cook CE, et al.Plant Antitumor Agent1The isolation and structure of Camptothecin,a novel alkaloidal Leukemia and tumor inhibitor from Camptotheca Acumianta[J].J Am Chem Soc, 1966, 88(16):3888-3890
[8] 朱关平等.生物转化喜树碱为10羟基喜树碱的研究[J]. 科学通报，1978, 23:761-764.
[9]. 徐淑云．中华临床药物学( 下册) ［M］．北京: 人民卫生出版，2003: 1470 － 1472．
[10]谢黎崖，吴永良，朱佩娟，等.载羟基喜树碱聚乳酸微球的制备与体外释药研究[J].中国新药杂志, 2012, 21(21):2557-2561
[11]洪海军.羟基喜树碱脂肪乳在大鼠体内的药动学及组织分布研究[J].中国药房, 2010, 21(13):1184-1186
[12]古锦辉, 凌家俊, 谢毅，等.羟基喜树碱脂质体的制备与性质研究[J].北方药学, 2012, 9(4):23-25
[13]罗颖, 李乐, 王衍戈，等.透析法制备载羟基喜树碱-聚乳酸纳米粒及其理化性质研究[J].厦门大学学报, 2010, 49(6):832-837
[14]刘璐, 姚日生, 尤启冬，等.微凝胶偶联羟基喜树碱的制备及性质[J].高分子材料科学与工程, 2011, 27(4):143-146
[15]ZHANG G，DING L， RENEGAR R， et al.Hydroxycamptothecin-loaded Fe3O4 nanoparticles induce human lung cancer cell apoptosis through caspase- 8 pathway activation and disrupt tight junctions[J].Cancer Sci, 2011, 102(6):1216-
[16]孙明磊，谢卫红，王昌美，等.平阳霉素-活性炭纳米微粒行口腔癌淋巴化疗的靶向性评价[J].华西口腔医学杂志, 2011, 29(3):253-256
[17]李振平，赵建江.平阳霉素量子点聚乳酸羟基乙酸纳米粒的制备[J].广东牙病防治, 2012, 20(5):245-250
[18] 陈渝萍，程江，张艳军，汪辛.叶酸特异性靶向活化态肝星状细胞的聚合物纳米载体及其药用用途.中国发明专利，申请号：201610018293.5.
[19]Guo X, Wei X, Jing Y, et al.Size Changeable Nanocarriers with Nuclear Targeting for Effectively Overcoming Multidrug Resistance in Cancer Therapy[J].Advanced Materials, 2015, 27(41):6450-
[20]Yang J, Liu F, Yang L, et al.Hydrolytic and enzymatic degradation of poly(trimethylene carbonate-co- d,l -lactide) random copolymers with shape memory behavior[J].European Polymer Journal, 2010, 46(4):783-791
[21]Endo K, Ueno T, Kondo S, et al.Tumor-targeted chemotherapy with the nanopolymer-based drug NC-6004 for oral squamous cell carcinoma [J][J].Cancer Sci, 2013, 104(3):369-374
[22]刘杰, 高福平, 唐劲天.肿瘤主动靶向磁性纳米粒子研究现状及其在肿瘤热疗中的应用[J].科技导报, 2010, 28(19):108-112
[23]姚东刚，孙考祥，慕宏杰.环孢素 - 嵌段共聚物胶束的制备及大鼠药动学研究[J].药学学报, 2009, 44(12):1410-1415
[24]. Danhier F, Lecoutufier N, Vroman B．Paclitaxel-loaded PEGylated PLGA-based nanopartieles：in vitro and in vivo evaluation[J]．J Control Release，2009，133：11-17．
[25]马文转，王金铃，屠鹏飞.黄芩苷纳米胶束的制备、表征及其对-细胞抑制作用的研究[J].中草药, 2015, 46(4):507-512
[26]锁爱丽，王何静，钱军民，等.纳米胶束共同递送和- 对-乳腺癌细胞的杀伤作用[J].西安交通大学学报, 2016, 37(4):536-542
[27]苑营芝，马敏，张丹，等.响应性聚合物胶束的制备、表征及对阿霉素的控制释放研究[J].化学研究, 2016, 27(5):630-635