川续断皂苷VI对大鼠正畸牙周组织改建影响的研究

›› 2018, Vol. 38 ›› Issue (5): 410-416.

川续断皂苷VI对大鼠正畸牙周组织改建影响的研究

陈佩佩¹,王旭霞¹,王媛²,崔金婕¹,马丹¹,张君¹

1. 山东大学口腔医学院
2. 浙江大学附属口腔医学院正畸科

收稿日期:2017-11-24 修回日期:2018-01-22 出版日期:2018-05-28 发布日期:2018-05-23
通讯作者: 张君 E-mail:zhangj@sdu.edu.cn
基金资助:
山东省科技发展计划课题;国家自然科学基金

Effect of Asperosaponin D on periodontium remodeling of orthodontic teeth movement in rats

Received:2017-11-24 Revised:2018-01-22 Online:2018-05-28 Published:2018-05-23

摘要/Abstract

摘要： ［摘要］目的探究局部注射川续断皂苷VI(ASD)是否通过促进大鼠正畸牙移动过程中破骨细胞分化而影响牙周组织改建。方法选择40只6周龄雌性Wistar大鼠，随机分为4组（10只/组）：A组：空白对照组(局部注射生理盐水)，B组：ASD低浓度组(局部注射5 mg/kg ASD)，C组ASD高浓度组(局部注射10 mg/kg ASD)，D组：前列腺素E2组(局部注射适宜浓度PGE2)。实验分别于第3、7、14、21及28天5个时间点处死2只大鼠，对大鼠上颌第一磨牙近中压力侧牙周组织进行TRAP染色以观察破骨细胞数及免疫组化以观察破骨细胞分化因子（ODF）表达情况。结果从第3天到第21天，4组上颌第一磨牙近中压力侧TRAP阳性细胞的数目及ODF的表达随加力时间不断增多，第28天时出现下降。从第7天开始，与A组相比，实验组破骨细胞数量和ODF表达均增加，B组比C组、D组减少且有统计学差异，C组与D组无统计学差异。结论局部注射ASD和适宜浓度的PGE2可有效促进压力侧牙周组织破骨细胞的分化，加快牙周改建。局部注射10 mg/kg ASD与PGE2效果基本相近，而稍低浓度ASD效果弱于PGE2和稍高浓度ASD。

关键词: 川续断皂苷VI , 正畸牙移动, 破骨细胞分化, 牙周改建

Abstract: Objective The aim of this study was to evaluate the effects of Asperosaponin D(ASD) on periodontium remodeling as well as the mechanism of effects on osteoclast. Methods In all, forty Wistar rats were selected and randomly divided to four groups (n=10). The control group (Group A) received no drug, the experimental Group B received a low concentration ASD (5 mg/kg) injection, the experimental Group C received a high concentration ASD (10 mg/kg) injection and group D received an appropriate concentration PGE2 (25μg/kg). The 5 groups of rats were sacrificed in batch on the 3st, 7th, 14th, 21st and 28th day with cardiac perfusion. Osteoclastic activity in the first molar periodontal tissue was evaluated by TRAP stainings and immunohistochemical staining. All statistical analysis was performed using SPSS software version 13.0. Results From 3rd to 21st, TRAP staining and immunohistochemical analysis of periodontium by the expression of ODF showed that the osteoclast numbers of every groups increased. From 7th days to 28th days, compared with group A, the number of osteoclast in the experimental group(Group B, C, D) increased. the number of osteoclast in group B was less than in group C and group D, and there were similar with group C and group D. Conclusions Local injection of both low and high concentrations of ASD and PGE2 may promote OTM by enhancing osteoclastic activity. The effect of local injection of high concentrations of ASD and PGE2 is similar, however The effect of high concentrations of ASD solution is more obvious than the effect of concentrations of ASD solition.

Key words: Asperosaponin D, orthodontic tooth movement, osteoclast differentiation, periodontium remodeling

中图分类号:

R783.5

陈佩佩王旭霞王媛崔金婕马丹张君. 川续断皂苷VI对大鼠正畸牙周组织改建影响的研究[J]. , 2018, 38(5): 410-416.

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