丹参素在体外条件下对口腔鳞癌细胞  CAL-27抑制作用的研究

›› 2019, Vol. 39 ›› Issue (2): 113-116.

丹参素在体外条件下对口腔鳞癌细胞 CAL-27抑制作用的研究

陈钱¹,林慧²,于敬伟³,李国林⁴

1. 哈尔滨医科大学附属第一医院口腔颌面外科
2. 哈尔滨医科大学附属口腔医院口腔颌面外科
3. 哈尔滨医科大学附属第一医院口腔医院
4. 哈尔滨医科大学附属口腔医院

收稿日期:2018-08-27 修回日期:2018-10-18 出版日期:2019-02-28 发布日期:2019-03-05
通讯作者: 李国林 E-mail:liguolin@126.com
基金资助:
超支化聚合物骨靶向药物递送系统的构建及相关评价;不同粒径纳米粒子对肿瘤转移淋巴结靶向效果的实时评价

Inhibitory effect of danshensu on oral squamous cell carcinoma CAL-27 cells in vitro

Received:2018-08-27 Revised:2018-10-18 Online:2019-02-28 Published:2019-03-05

摘要/Abstract

摘要： [摘要] 目的探讨丹参素在体外条件下对口腔鳞癌细胞CAL-27的增殖、迁移及侵袭能力的影响。方法丹参素处理CAL-27细胞，MTT法检测丹参素对CAL-27细胞活性的影响。划痕实验检测丹参素对CAL-27细胞的迁移能力的作用。Transwell实验检测其对细胞的侵袭能力的作用。结果 MTT显示，在丹参素浓度为40 μg/mL、60 μg/mL和80 μg/mL时，丹参素对CAL-27细胞增殖的抑制可呈剂量和时间依赖性。划痕实验中，24h组与对照组相比，具有显著差异性（P<0.01）。Transwell侵袭实验结果表明，丹参素可降低CAL-27细胞的侵袭能力。结论在体外条件下，丹参素在一定浓度范围内可抑制CAL-27细胞的增殖，降低CAL-27细胞的迁移及侵袭能力。

关键词: 丹参素, 口腔鳞癌, 增殖, 迁移, 侵袭

Abstract: Abstract： Objective To investigate the effects of Danshensu on the proliferation, migration and invasion of oral squamous cell carcinoma CAL-27 in vitro. Methods Danshensu was used to treat CAL-27 cells, and the effect of Danshensu on the activity of CAL-27 cells were detected by MTT assay. The scratch test determined the effect of Danshensu on the migration ability of CAL-27 cells. The transwell assay inspected its effect on the invasive ability of cells.Results MTT showed that Danshensu inhibited the proliferation of CAL-27 cells in a dose- and time-dependent manner at concentrations of Danshensu at 40 μg/mL, 60 μg/mL and 80 μg/mL. In the scratch test, the difference between the 24h group and the control group was statistically significant. (P<0.01). The results of Transwell invasion assay showed that Danshensu can reduce the invasive ability of CAL-27 cells. Conclusion In vitro ,danshensu inhibits the proliferation of CAL-27 cells and reduces the migration and invasion of CAL-27 cells.

Key words: Danshensu, oral squamous cell carcinoma, proliferation, migration, invasion

中图分类号:

R739.8

陈钱林慧于敬伟李国林. 丹参素在体外条件下对口腔鳞癌细胞 CAL-27抑制作用的研究[J]. , 2019, 39(2): 113-116.

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