转化生长因子β3促进兔牙髓干细胞成骨向分化机制的初步研究

›› 2019, Vol. 39 ›› Issue (2): 97-102.

• 基础研究 • 下一篇

转化生长因子β3促进兔牙髓干细胞成骨向分化机制的初步研究

艾力麦尔旦·艾尼瓦尔¹,木合塔尔·霍加²

1. 新疆维吾尔自治区人民医院口腔科
2. 深圳市罗湖区人民医院

收稿日期:2018-08-30 修回日期:2018-10-09 出版日期:2019-02-28 发布日期:2019-03-05
通讯作者: 艾力麦尔旦·艾尼瓦尔 E-mail:trailblazer1006@163.com
基金资助:
国家自然科学基金

Preliminary study on the mechanism of TGF-β3 in promoting osteogenic differentiation of rabbit dental pulp stem cells

,Muhetaer Huojia

Received:2018-08-30 Revised:2018-10-09 Online:2019-02-28 Published:2019-03-05
Supported by:
Natural Science Foundation of China

摘要/Abstract

摘要： ［摘要］目的探讨转化生长因子β3（transforming growth factor β3,TGF-β3 ）体外联合兔牙髓干细胞(rabbit dental pulp stem cells,rDPSCs)诱导其成骨向分化的作用机制。方法酶解组织块法分离获得rDPSCs；将rDPSCs分为实验组（rDPSCs+80ng/ml TGF-β3）,对照组（rDPSCs+矿化液）和空白组（rDPSCs）,三组细胞分别培养第7、14d行ALP活性定量检测；RT—qPCR检测COL-IA1、Runx-2, OPN, BSP和Smad4基因的表达；western blot法检测COL-1A1,Runx-2蛋白的表达情况；茜素红染色观察矿化结节形成情况。结果成功分离获得rDPSCs；实验组ALP含量明显高于对照组和空白组（P＜0.05）；COL-1A1、Runx-2、OPN、BSP、Smad4基因的表达均高于空白组（P＜0.05）COL-1A1、Smad4基因的表达高于对照组（P＜0.05）; Western blot结果示实验组COL-1A1,Runx-2蛋白的表达均强于其余两组（P＜0.05）；实验组矿化结节较其余两组明显。结论 TGF-β3体外促进rDPSCs成骨向分化；TGF-β3促进rDPSCs成骨向分化可能通过激活Smad通路来实现。

关键词: 转化生长因子β3, 兔牙髓干细胞, 成骨向分化, Smad通路

Abstract: Abstract：0bjective To investigate the effect and the mechanism of TGF-β3 on the osteogenic differentiation of rabbit dental pulp stem cells(rDPSCs).Methods rDPSCs in third passage were divided into the test group(rDPSCs+80ng/ml TGF-β3),control group (rDPSCs+osteogenic medium)and blank group(rDPSCs)；For each group after 7,14d, ALP activity was measured, RT—qPCR was carried out to measure the expression of COL-1A1,Runx-2,OPN,BSP and Smad4,and alizarin red S staining(ARS) was held after14,21 day to testify mineralized matrix productivity. Results ALP activity showed significant difference in each two groups（P＜0.05）. Experimental group showed higher expression of COL-1A1,Runx-2,OPN,BSP and Smad4 than the blank group（P＜0.05） and higher expression of COL-1A1 and Smad4 than the control group（P＜0.05）. Conclusion TGF-β3 can improve the osteogenic differentiation of rDPSCs and this process maybe through activating Smad pathway.

Key words: transforming growth factor-β3（TGF-β3）, rabbit dental pulp stem cells(rDPSCs), osteogenic differentiation, Smad pathway

艾力麦尔旦·艾尼瓦尔木合塔尔·霍加. 转化生长因子β3促进兔牙髓干细胞成骨向分化机制的初步研究[J]. , 2019, 39(2): 97-102.

Muhetaer Huojia. Preliminary study on the mechanism of TGF-β3 in promoting osteogenic differentiation of rabbit dental pulp stem cells[J]. , 2019, 39(2): 97-102.

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转化生长因子β3促进兔牙髓干细胞成骨向分化机制的初步研究

Preliminary study on the mechanism of TGF-β3 in promoting osteogenic differentiation of rabbit dental pulp stem cells

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