长链非编码RNA MEG3对头颈鳞状细胞癌增殖和侵袭的影响

›› 2019, Vol. 39 ›› Issue (5): 400-403.

长链非编码RNA MEG3对头颈鳞状细胞癌增殖和侵袭的影响

季叶枫¹,于洋苗利民杨建荣²,袁华³

1. 江苏省口腔医院
2. 南京医科大学口腔医学研究所，南京医科大学口腔医院
3. 南京医科大学附属口腔医院

收稿日期:2018-11-19 修回日期:2018-12-29 出版日期:2019-05-28 发布日期:2019-05-21
通讯作者: 杨建荣 E-mail:jrjskq@163.com
基金资助:
国家自然科学基金;国家自然科学基金;江苏高校优势学科建设工程资助项目;江苏省科教强卫工程青年医学人才项目

Influences of long non-coding RNA MEG3 on the proliferation and invasion of HNSCC

Received:2018-11-19 Revised:2018-12-29 Online:2019-05-28 Published:2019-05-21

摘要/Abstract

摘要： 目的：探究长链非编码ＲNA母系表达基因3(lncRNA MEG3) 对头颈部鳞癌细胞增殖和侵袭的影响。方法：体外培养头颈部鳞癌细胞系Cal27转染MEG3,分为MEG3阴性对照组( MEG3对照组) 、MEG3慢病毒过表达转染组( MEG3 过表达组)。实时荧光定量PCR( qRT-PCR) 检测MEG3 mRNA表达; CCK-8法检测细胞增殖情况；transwell检测细胞迁移情况; 蛋白免疫印迹法(western blotting WB) 检测细胞中ZEB2 、波形蛋白( vimentin) 、E-钙黏着蛋白( E-cadherin) 蛋白表达情况。结果:在Cal27细胞系中，与MEG3对照组相比，MEG3过表达组细胞中MEG3mＲNA 表达升高( P＜0.05) 。与阴性对照组相比，MEG3过表达组细胞迁移、侵袭数量均降低( P＜0．05) 。与阴性对照组相比，MEG3 转染组ZEB2、vimentin 蛋白表达降低，E-cadherin蛋白表达升高( P＜0.05) 。结论：MEG3 表达升高后可抑制头颈部鳞癌细胞的增殖、侵袭，其机制可能与抑制细胞上皮－间充质转化有关。

关键词: 头颈部鳞癌（HNSCC）, 母系表达基因3（MEG3）, 增殖, 侵袭, 上皮-间充质转化（EMT）

Abstract: Abstract Objective: To investigate the effects of long non-coding RNA maternally expression gene 3 (MEG3) on proliferation and invasion of head and neck squamous cell carcinoma (HNSCC). Methods: Cultured the head and neck squamous cell carcinomacell line Cal27 in vitro，and they were divided into negative transfection group (MEG3 control ) and MEG3 Lentivirus overexpression transfection group (MEG3 overexpression) after the transfection of MEG3，real-time quantitative PCR(qRT－PCR) was used to detect MEG3 mRNA expression，CCK-8 assay was used to detect cell proliferation; transwell was used to detect cell migration; western blotting (WB) was used to detect the expressions of ZEB2，Vimentin and E-cadherin in cells．Results: In Cal27 cell， MEG3 mRNA expression was increased in the MEG3 overexpression group compared with the MEG3 control group (P<0.05).Compared with the control group, cell migration and invasion in the MEG3 overexpression group were decreased (P＜0.05)．Compared with control group，the expressions of ZEB2 and vimentin protein in MEG3 overexpression group were decreased，and the expression of E-cadherin protein was increased (P＜0.05)．Conclusion: The increase of MEG3 expression can inhibit the proliferation and invasion of head and neck squamous cell carcinomacells，the mechanism may be related to the inhibition of epithelial cell mesenchymal transition ( EMT)．

Key words: head and neck squamous cell carcinoma (HNSCC), maternally expression gene 3 (MEG3), proliferation, invasion, epithelial cell mesenchymal transition （EMT)

中图分类号:

R739.9

季叶枫于洋苗利民杨建荣袁华. 长链非编码RNA MEG3对头颈鳞状细胞癌增殖和侵袭的影响[J]. , 2019, 39(5): 400-403.

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