介孔二氧化硅包覆金纳米双锥近红外光响应药物载体的构建及抗菌性能

›› 2019, Vol. 39 ›› Issue (5): 404-408.

介孔二氧化硅包覆金纳米双锥近红外光响应药物载体的构建及抗菌性能

林坚¹,何智琪¹,周云龙²,邓辉¹

1. 温州医科大学附属口腔医院
2. 温州生物材料与工程研究所

收稿日期:2018-12-19 修回日期:2019-01-30 出版日期:2019-05-28 发布日期:2019-05-21
通讯作者: 邓辉 E-mail:dh0726@163.com
基金资助:
国家自然科学基金项目;浙江省医药卫生科技计划项目;浙江省自然科学基金项目

Mesoporous silica coated Au Nanobipyramids nanoparticle for near-infrared responsive drug delivery system and its application in antibacteria

Received:2018-12-19 Revised:2019-01-30 Online:2019-05-28 Published:2019-05-21

摘要/Abstract

摘要： 目的　制备介孔二氧化硅包覆金纳米双锥的药物载体，研究其表征、生物相容性、载药性能、近红外光响应性能及抗菌性能。方法　运用晶种介导方法合成金纳米双锥，并在其表面包覆介孔二氧化硅，采用扫描电镜、透射电镜和紫外分光光度计对其进行表征，并通过MTT实验检测其生物相容性；观察其米诺环素负载率及近红外光照射对其药物累积释放率的影响，探讨其抗菌效果。结果　成功制备介孔二氧化硅包覆金纳米双锥药物载体，透射电镜下可见无序的孔隙结构，对细胞增殖无明显影响，药物负载率为76.5%，经近红外光照射后药物累积释放量上升41.7%。体外抗菌实验显示显著抑制细菌生长达90%。结论　介孔二氧化硅包覆金纳米双锥药物载体具有良好生物相容性、近红外光响应药物释放及抗菌作用，在牙周局部缓控释抗菌中有应用的潜能。

关键词: 牙周炎, 金纳米双锥, 介孔二氧化硅, 近红外光响应, 抗菌性能

Abstract: Objective　 To prepare the drug delivery system of mesoporous silica coated Au Nanobipyramids(Au NBPs@SiO2), and evaluate its characterization, biocompatibility, drug loading, near-infrared responsive property and antibacterial properties. Methods　 The Au Nanobipyramids (Au NBPs) were synthesized by a seed-mediated method, and coated with mesoporous silica (MSNs). It was characterized by scanning electron microscopy, transmission electron microscopy and ultraviolet spectrophotometer, and its biocompatibility was tested by MTT assay. Then, to investigate the Near-Infrared responsive drug release behavior. Finally, the antibacterial properties was evaluated by using E.coli. Results　The drug delivery system of Au NBPs@SiO2 was successfully prepared. The disordered pore structure was observed under transmission electron microscope. And it had no obvious effect on cell proliferation. The loading efficiency reached 76.5%, and the cumulative drug release increased by 41.7% under NIR irradiation. In vitro antibacterial experiments showed it had significantly antibacterial effects. Conclusions　 The drug delivery system of Au NBPs@SiO2 had good biocompatibility, near-infrared responsive drug release behavior and antibacterial properties, and had potential application in periodontal sustained-release antibacterial therapy.

Key words: periodontitis, Au nanobipyramids, mesoporous silica, nearinfrared responsive, antibacterial property

中图分类号:

R781.42

林坚何智琪周云龙邓辉. 介孔二氧化硅包覆金纳米双锥近红外光响应药物载体的构建及抗菌性能[J]. , 2019, 39(5): 404-408.

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