CpG 2006对大鼠实验性牙移动骨改建影响的研究

摘要/Abstract

摘要： 目的探究CpG 2006对大鼠实验性牙移动及牙槽骨改建的影响。方法选择雄性Wistar大鼠，建立上颌牙齿移动模型，左侧作为实验组，于第一磨牙颊黏膜下局部注射CpG 2006，右侧作为对照组，对应部位注射等量PBS。每3天给药一次，14 d后处死大鼠，测量大鼠上颌第一磨牙移动距离，并拍摄X线片，采用Realtime PCR技术检测牙周组织中成骨、破骨及炎症相关因子表达，通过HE染色检测CpG 2006对各脏器的影响。结果大鼠实验侧第一磨牙移动距离明显小于对照侧（P<0.05），实验侧牙周组织中Runx2、SP7、OCN mRNA表达水平高于对照侧（P<0.01），而IL1、IL6、TNFα mRNA表达水平明显低于对照侧（P< 0.01），双侧Nfatc、CTSK、MMP9 mRNA表达水平无统计学差异，脏器组织学检测未见明显异常。结论 CpG 2006可通过促进牙周组织成骨活化来调控牙齿移动，并降低该过程中炎症因子的表达。

关键词: CpG 2006, 成骨, 炎症, 正畸牙移动, 大鼠

Abstract: Objective To investigate the influence of CpG 2006 on tooth movement and alveolar bone remodeling in rats. Methods Male Wistar rats were chosen. Orthodontic tooth movement devices were installed at both maxillary of each rat, and the left side was chosen to be the treatment lateral, while the right side the control lateral. CpG 2006 or PBS was injected under the buccal mucosa of the maxillary first molar. Injection repeated every 72 h to all the animals sacrificed 14 days later. The migration distance of the molar was measured depending on specimen and Xray. The expression levels of osteoclast and inflammatory related factors were detected by Realtime PCR. HE staining was used for detecting the effects of CpG 2006 on the organ. Results The migration distance of the molar in treatment lateral was smaller and the expression levels of IL1,IL6,TNFɑ mRNA were lower than the control lateral, while the expression levels of Runx2,SP7,OCN mRNA were higher than the control lateral, and there were no differences among the expression levels of Nfatc,CTSK, MMP9 mRNA between the two laterals. No pathological changes were found in their organs. Conclusion CpG 2006 can regulate the distance of tooth movement by promoting the osteogenic activation of periodontal tissue, and reduce the expression of inflammatory factors during tooth movement.

Key words: CpG 2006, osteogenesis, inflammation, orthodontic tooth movement, rats

中图分类号:

R783.5

秦甜甜于文雯汪洋刘宇妍黄蕾周雪纯孙新华. CpG 2006对大鼠实验性牙移动骨改建影响的研究[J]. , 2019, 39(9): 769-773.

参考文献 23

[1]	Krishnan V, Davidovitch Z, et al.Cellular,molecular,and tissure-level reactions to orthodontic force[J].American Journal of Orthodontics and Dentofacial Orthopedics, 2006, 129(4):469e1-469e32
[2]	Ildeu Andrade Jr; Silvana R.A. Taddei,et alInflammation and Tooth Movement: The Role of Cytokines,Chemokines,and Growth Factors[J].Seminars in Orthodontics, 2012, 18(4):257-269
[3]	赵云鹤, 李娇, 雷浪, 李厚轩.牙槽骨微穿孔术促进大鼠正畸牙移动的机制研究[J].口腔医学研究, 2018, 34(09):960-963
[4]	Ge MK, He WL, Chen J, et al.Efficacy of low-level laser therapy for accelerating tooth movement during orthodontic treatment:a systematic review and meta-analysis[J].Lasers Med Sci, 2015, 30(5):1609-1618
[5]	Iglesias-Linares A, Moreno-Fernandez AM, Yaez-Vico R, et al.The use of gene therapy vscorticotomy surgery in accelerating orthodontic tooth movement[J].Orthod Craniofac Res, 2011, 14(3):138-148
[6]	朱文倩, 唐国华.药物对正畸牙移动的影响[J].口腔医学研究, 2013, 29(06):590-592
[7]	Dileo J, Banerjee R, et al.Lipid-protamine-DNA-mediated antigen delivery to antigen-presenting cells results in enhanced anti-tumor immune responses[J].Molecular Therapy, 2003, 7(5):640-648
[8]	Suman Gupta; Shraddha A.Sane, et al.CpG Oligodeoxynucleotide 2006 and Miltefosine, a Potential Combination for Treatment of Experimental Visceral Leishmaniasis [J].Antimicrobial Agents and Chemotherapy, 2011, 55(7):3461-3464
[9]	Ellis RD, Martin LB, Shaffer D, et al.Phase 1 trial of the Plasmodium falciparum blood stage vaccine MSP1(42)-C1 Alhydrogel with and without CPG 7909 in malaria na?ve adults[ J][J].PLoSOne, 2010, 5(1):e8787-e8787
[10]	Abe N, Kodama S, Hirano T, et al.Nasal vaccination with CpG oligodeoxynucleotide induces protective immunity against non-typeable Haemophilus influenzae in the nasopharynx [ J ][J].Laryngoscope, 2006, 116(3):407-412
[11]	Shen Y，Feng Z，Lin C，et al.An oligodeoxynucleotide that induces differentiation of bone marrow mesenchymal stem cells to osteoblasts in vitro and reduces alveolar bone loss in rats with periodontitis[J].Int J Mol Sci, 2012, 13(3):2877-2892
[12]	Feng ZY, Shen YQ, Wang L, Cheng L, Wang J, Li Q, Shi W, Sun X.An oligodeoxynucleotide with promising modulation activity for the proliferation and activation of osteoblast[J].International Journal of Molecular Sciences, 2011, 12(4):2543-2555
[13]	王静, 孙新华, 冯志远, 申玉芹.特定寡核苷酸对大鼠实验性牙移动的影响[J].吉林大学学报医学版, 2011, 37(05):812-816
[14]	Hartmann, Gunther; Weeratna, Risini D.et alDelineation of a CpG Phosphorothioate Oligodeoxynucleotide for Activating Primate Immune Responses In Vitro and In Vivo[J].Journal of Immunology, 2000, 164(3):1617-1624
[15]	Hartmann, Gunther; Krieg, Arthur M.et alMechanism and Function of a Newly Identified CpG DNA Motif in Human Primary B Cells[J].Journal of Immunology, 2000, 164(2):944-953
[16]	Wilson Heather L, Dar Arshud et al.Immune Mechanisms and Therapeutic Potential of CpG Oligodeoxynucleotides[J].International Reviews of Immunology, 2006, 25(3-4):183-213
[17]	Blazar BR, Krieg AM, Taylor PA.et alSynthetic unmethylated cytosine-phosphate-guanosine oligodeoxynucleotidesare potent stimulators of antileukemia responses in naive and bone marrow transplant recipients[J].BLOOD, 2001, 98(4):1217-1225
[18]	Rashid H, Ma C, Chen H, et al.Sp7 and Runx 2 molecular complex synergistically regulate expression of target genes[J].Connect Tissure Res, 2004, 55(Suppl 1):83-87
[19]	Ren DP, Wei FL, Hu LH, et al.Phosphorylation of Runx2, Induced by Cyclic Mechanical Tension via ERK1/2 Pathway, Contributes to Osteodifferentiation of Human Periodontal Ligament Fibroblasts[J].Journal of Cellular Physiology, 2015, 230(10):2426-2436
[20]	Masako Fujioka-Kobayashi; Jordi Caballé-Serrano; Dieter D.Bosshardt; Reinhard Gruber; Daniel Buser; Richard J. Miron Bone conditioned media (BCM) improves osteoblast adhesion and differentiation on collagen barrier membranes [H].BMC Oral Health, 2016, 17(1):7.
[21]	Tian J, Qi W, Zhang Y, et al.Bioaggregate Inhibits Osteoclast Differentiation,Fusion,and Bone Resorption In Vitro[J].J Endod, 2015, 41(9):1500-1506
[22]	Roberts WE and Ferguson DJ.Cell kinetics of periodontal ligament[M].Boca Raton, Fla:CRC Press, 1995
[23]	Nonnenmacher C, Dalpke A, Zimmermann S, et al.DNA from periodontopathogenic bacteria is immunosimulatory for mouse and human immune cells. [J] Infect Immun. 2003, 71(2): 850-856.[J].Infection and Immunity, 2003, 71(2):850-856