基质金属蛋白酶9在慢性牙周炎及类风湿关节炎中的水平及相关性分析

›› 2019, Vol. 39 ›› Issue (9): 793-798.

基质金属蛋白酶9在慢性牙周炎及类风湿关节炎中的水平及相关性分析

赵莹¹,靳赢¹,任宇舒¹,宋利明²,李健³,林晓萍¹

1. 中国医科大学附属盛京医院
2. 辽宁省沈阳市中国医科大学附属盛京医院
3. 中国医科大学附属口腔医院

收稿日期:2019-01-24 修回日期:2019-04-10 出版日期:2019-09-28 发布日期:2019-09-17
通讯作者: 林晓萍 E-mail:xiaoping_ba@126.com
基金资助:
国家自然科学基金;中华口腔医学会专项基金

Correlation analysis of matrix metalloproteinase 9 in chronic periodontitis and rheumatoid arthritis

¹,Ying JIN ³,

Received:2019-01-24 Revised:2019-04-10 Online:2019-09-28 Published:2019-09-17

摘要/Abstract

摘要： 目的　检测慢性牙周炎患者(chronic periodontitis, CP)和类风湿关节炎(rheumatoid arthritis，RA)患者龈沟液及血清中基质金属蛋白酶9(MMP-9)的含量，探讨MMP-9与CP及RA之间的相关关系。方法:按纳入标准选取RA患者(RA组)23人、CP患者(CP组)18人、CP伴RA患者(CP+RA组)24人和健康对照组(H组)16人；记录4组受试者一般信息、牙周探诊深度（PD）、临床附着丧失(CAL)、、龈沟出血指数(SBI)、简化牙石指数(CI-S)、简化软垢指数(DI-S);收集各组受试者龈沟液及血清，采用ELISA法检测分析龈沟液及血清中的MMP-9的表达水平，并分析MMP-9与牙周指标的相关性。采用SPSS20.0软件包对数据进行统计学分析。结果：CP+RA组CAL、SBI、DI-S高于RA组、CP组与H组 (P?0.05)。血清中MMP-9的水平CP+RA组(2671.71±1258.33μg/L)高于RA组(1444.54±770.03μg/L)、CP组(492.41±485.04 μg/L)、H组(67.14±34.95μg/L) (P?0.05)。龈沟液中MMP-9的水平CP+RA组(26.11±5.25μg/L)高于CP组(18.83±11.87μg/L)及H组(1.04±0.58μg/L) (P?0.05)。CP+RA组CAL 、SBI与血清中MMP-9的表达呈正相关，CP组CI-S、DI-S与血清中MMP-9表达呈正相关(P?0.05)。结论：CP与RA之间具有相关性，MMP-9在CP和RA中表达显著升高，MMP-9可能与CP和RA的发生发展存在相关关系。

关键词: 牙龈卟啉单胞菌血凝素2, HA2, 牙龈卟啉单胞菌, 牙周炎

Abstract: Objective　 To detect the contents of matrix metalloproteinase 9(MMP-9) in gingival groove fluid and serum of patients with chronic periodontitis (CP) and rheumatoid arthritis (RA), and discuss the correlation between MMP-9, CP and RA. Methods According to the inclusion criteria, 23 patients with rheumatoid arthritis (RA), 18 patients with chronic periodontitis (CP), 24 patients with chronic periodontitis and rheumatoid arthritis (CP+RA) and 16 healthy control group (H) were selected. The general conditions of patients were recorded, including the probing depth (PD), clinical attachment loss (CAL), sulcus bleeding index (SBI), simplified dental calculus index (CIS), and simplified debris index (DIS). Gingival crevicular fluid and serum were collected from each group. The expression level of MMP-9 in gingival crevicular fluid (GCF) and serum was detected by ELISA. The correlation between MMP-9 and periodontal index was analyzed. SPSS20.0 software package was used for statistical analysis of the data. Results The values of CAL, SBI and DI-S in RA+CP group were higher than RA group, CP group and H group. The level of MMP-9 in serum in CP+RA group (2671.71±1258.33μg/L) was higher than that in the RA group (1444.54±770.03μg/L), CP group (511.38±539.52μg/L), and H group (67.14±34.95μg/L) (P?0.05). The level of MMP-9 in the gingival crevicular fluid was higher in CP+RA group (26.11±5.25μg/L) than in the CP group (18.83±11.87μg/L) and H group (1.04±0.58μg/L) (P?0.05). There was a positive correlation between CAL and SBI in CP+RA group and MMP-9 expression in serum, CI-S and DI-S in CP group were positively correlated with serum MMP-9 . Conclusion There is a correlation between CP and RA, and the expression of MMP-9 significantly increases in CP and RA. MMP-9 may be related to the occurrence and development of CP and RA.

Key words: Porphyromonas gingivalis hemagglutinin 2, HA2, Porphyromonas gingivalis, Periodontitis

中图分类号:

R781.4

赵莹靳赢任宇舒宋利明李健林晓萍. 基质金属蛋白酶9在慢性牙周炎及类风湿关节炎中的水平及相关性分析[J]. , 2019, 39(9): 793-798.

Ying JIN. Correlation analysis of matrix metalloproteinase 9 in chronic periodontitis and rheumatoid arthritis[J]. , 2019, 39(9): 793-798.

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