消退素E1对氧化应激下人牙髓干细胞生物学性能的影响

›› 2020, Vol. 40 ›› Issue (12): 1061-1066.

• 基础研究 • 下一篇

消退素E1对氧化应激下人牙髓干细胞生物学性能的影响

杨婷,陶硕,张旗

同济大学口腔医（学）院

收稿日期:2020-05-29 修回日期:2020-08-04 出版日期:2020-12-28 发布日期:2020-12-22
通讯作者: 张旗 E-mail:qizhang@tongji.edu.cn
基金资助:
骨保护素在牙根吸收中的多重保护作用和机制的研究;牙周膜成纤维细胞外泌体递送的骨保护素对再植牙根吸收的保护作用及机制研究

Effects of Resolvin E1 on the biological performance of human dental pulp stem cells under oxidative stress

Ting Yang¹,Shuo TAO²,

Received:2020-05-29 Revised:2020-08-04 Online:2020-12-28 Published:2020-12-22

摘要/Abstract

摘要： [摘要] 目的探讨消退素E1（Resolvin E1,RvE1）对过氧化氢（hydrogen peroxide,H2O2）诱导的氧化应激下人牙髓干细胞（human dental pulp stem cells,hDPSCs）生物学性能的影响。方法采用酶解组织块法体外分离培养hDPSCS，免疫荧光染色检测hDPSCs中RvE1受体ChemR23的表达情况；hDPSCs随机分为3组，对照组、H2O2组和H2O2+RvE1组，H2O2组和H2O2+RvE1组加入200μM H2O2预处理建立细胞氧化应激模型，24h后，对照组和H2O2组换用完全培养基继续培养，H2O2+RvE1组则换用加入100nMRvE1的完全培养基继续培养，苏木素-伊红（HE）染色和鬼笔环肽染色观察hDPSCs形态学改变；cck-8、划痕实验、碱性磷酸酶（Alkaline phosphatase,ALP）染色分别检测hDPSCs增殖、迁移和成牙向分化能力的改变。结果 ChemR23在hDPSCs中广泛表达；与对照组相比，H2O2组的hDPSCs形态异常，增殖、迁移和成牙向分化能力显著降低（P<0.05），与H2O2组相比，H2O2+RvE1组的hDPSCs增殖、迁移和分化能力明显增强（P<0.05）。结论 RvE1促进氧化应激下的hDPSCs增殖、迁移及成牙向分化。

关键词: 消退素E1, 牙髓干细胞, 氧化应激

Abstract: [Abstract] Objective: To investigate the effect of Resolvin E1 (RvE1) on the biological performance of human dental pulp stem cells (hDPSCs) under hydrogen peroxide-induced (H2O2) oxidative stress. Methods: The hDPSCS were isolated and cultured in vitro by enzymatic tissue block method, and the expression of RvE1 receptor ChemR23 in hDPSCs was detected by immunofluorescence staining; hDPSCs were randomly divided into 3 groups, control group, H2O2 group and H2O2+RvE1 group, H2O2 group and H2O2+RvE1 group were pretreated with 200μM H2O2 for 24h to establish a model of cell oxidative stress. After 24h, the control group and H2O2 group continued to use complete culture medium, and the H2O2+RvE1 group continued to use complete medium containing 100nMRvE1. Hematoxylin-eosin(HE) staining and phalloidin staining were used to observe the morphological changes of hDPSCs; cck-8, scratch test and alkaline phosphatase (ALP) staining were used to detect the changes of hDPSCs proliferation, migration and odontogenic differentiation ability. Results: ChemR23 was widely expressed in hDPSCs; Compared with the control group, hDPSCs in the H2O2 group had abnormal morphology and the ability of proliferation, migration, and odontogenic differentiation was significantly decreased (P <0.05). Compared with the H2O2 group, hDPSCs in the H2O2+RvE1 group had significantly enhanced ability of proliferation, migration and odontogenic differentiation(P <0.05). Conclusion: RvE1 promotes the proliferation, migration and odontogenic differentiation of hDPSCs under oxidative stress.

Key words: resolvin E1, dental pulp stem cells, oxidative stress

中图分类号:

R349.5

杨婷陶硕张旗. 消退素E1对氧化应激下人牙髓干细胞生物学性能的影响[J]. 口腔医学, 2020, 40(12): 1061-1066.

Ting Yang Shuo TAO. Effects of Resolvin E1 on the biological performance of human dental pulp stem cells under oxidative stress[J]. Stomatology, 2020, 40(12): 1061-1066.

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消退素E1对氧化应激下人牙髓干细胞生物学性能的影响

Effects of Resolvin E1 on the biological performance of human dental pulp stem cells under oxidative stress

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