牙周炎微环境下静态牵张力对PDLSCs炎症因子分泌的影响

›› 2020, Vol. 40 ›› Issue (12): 1079-1083.

牙周炎微环境下静态牵张力对PDLSCs炎症因子分泌的影响

蔡东轩¹,孙唯夫²,金作林³,刘佳³

1. 第四军医大学
2. 第四军医大学第三附属医院
3. 空军军医大学第三附属医院

收稿日期:2020-06-22 修回日期:2020-07-27 出版日期:2020-12-28 发布日期:2020-12-22
通讯作者: 蔡东轩 E-mail:1091724478@qq.com
基金资助:
国家自然科学基金青年项目

The effect of static mechanical train on the inflammatory cytokines reactions of PDLSCs in periodontitis microenvironment

Received:2020-06-22 Revised:2020-07-27 Online:2020-12-28 Published:2020-12-22
Contact: Dong-Xuan CAI E-mail:1091724478@qq.com
Supported by:
Youth Program of National Natural Science Foundation of China

摘要/Abstract

摘要： 目的　探究不同大小静态牵张力（Static mechanical strain, SMS）对正常来源的牙周膜干细胞（Periodontal ligament stem cells obtained from healthy periodontal tissues, HPDLSCs）和牙周病组织来源的牙周膜干细胞（Periodontal ligament stem cells obtained from periodontal tissues of periodontitis patients, PPDLSCs）炎症因子表达的影响。方法　以HPDLSCs和PPDLSC为对象构建细胞体外培养－力学刺激模型，采用Flexcell牵张力加载装置，分别给予静态牵张力( 频率为 0.1Hz，力值设定为6%，8%，10%，12%和14%) 刺激12 h，并设立正常对照组。采用Elisa检测SMS加载后HPDLSCs和PPDLSCs中炎症因子IL-1β，TNF-α，IL-6 和IL-8分泌水平。结果　未加力情况下PPDLSCs中IL-1β，TNF-α，IL-6 和IL-8分泌水平均高于HPDLSCs（P<0.05），加载SMS后PPDLSCs中IL-1β和TNF-α的表达水平略有升高（P<0.05），而IL-6 和IL-8的表达水平则大幅提升，且在一定的范围内与SMS的大小呈正相关。结论　牙周炎微环境导致PDLSCs中炎症因子的表达情况增强且不同的炎症因子对SMS的敏感性有差异，其中IL-6 和IL-8表达量明显升高，提示其可能参与了牙周炎微环境下PDLSCs对SMS的反应。

关键词: 牙周炎微环境, 静态牵张力, 牙周膜干细胞, 炎性细胞因子

Abstract: Objective　To investigate the effect of different magnitudes of SMS on the inflammatory cytokines reactions of HPDLSCs and PPDLSCs. Methods　In vitro culture-tensile stimulation models of HPDLSCs and PPDLSCs were established using a Flexcell Tension Unit. SMS was applied on the HPDLSCs and PPDLSCs (the load was set for 6%, 8%, 10%, 12%, 14%, with frequency of 0.1 Hz.) for 12hours. At the same time，the normal control group was established. The secretions of inflammatory cytokines IL-6，IL-8，IL-1β and TNF-α were examined by elisa. Results　The secretion of IL-6, IL-8, IL-1β and TNF-α were higher in PPDLSCs than in HPDLSCs without SMS(P<0.05). When SMS was loaded, the expressions of IL-1β and TNF-α increased a little in PPDLSCs(P<0.05). However, the expression of IL-6 and IL-8 was up regulated to a considerable high level and had a positive correlation with the magnitude of SMS in a certain range in PPDLSCs. Conclusions　The periodontitis microenvironment increased the secretion of inflammatory cytokines of PDLSCs. Under SMS, different inflammatory cytokines were up-regulated by different extents, among which IL-6 and IL-8 were increased considerably, which indicates that IL-6 and IL-8 were involved in the reaction of PDLSCs to SMS in periodontitis microenvironment.

Key words: periodontitis microenvironment, static mechanical strain, periodontal ligament stem cells, inflammatory cytokine 

中图分类号:

R781.42

蔡东轩孙唯夫金作林刘佳. 牙周炎微环境下静态牵张力对PDLSCs炎症因子分泌的影响[J]. 口腔医学, 2020, 40(12): 1079-1083.

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