口腔扁平苔藓黏膜病损区微生物群落分析

摘要/Abstract

摘要： 目的　研究口腔扁平苔藓患者（OLP）相关微生物群的多样性，组成和结构。方法　利用无菌拭子对12名斑纹型OLP患者、12名糜烂型OLP患者和8名正常志愿者的颊或舌黏膜样本进行了16S rDNA基因分析，初步探讨OLP患者黏膜拭子样本微生物群的生态失调。结果　OLP组的微生物群与对照组不同，艾肯菌属（Eikenella）、小链菌属（Alysiella）、脓肿分枝杆菌（morococcus cerebrosus）、马氏普雷沃氏菌（Prevotella marshii）、颊纤毛菌C-1013-b ( Leptotrichia buccalis C-1013-b)和苛养颗粒链球菌（Granulicatella elegans）的相对丰度在OLP组显著较对照组高，其中，马氏普雷沃氏菌的相对丰度在糜烂型OLP组显著较斑纹型OLP组高（P=0.0009）。土壤杆菌属（Sediminibacterium）和唾液链球菌唾液亚种(Streptococcus salivarius subsp. Salivarius)的相对丰度在正常对照组中增加。结论　结果表明OLP组的相关微生物特征与对照组存在差异，为了确定OLP的有效预防和治疗策略，进一步研究OLP相关微生物群落至关重要。

关键词: 口腔扁平苔藓, 微生物群落, 16s rDNA基因, 拭子

Abstract: Objective　 The purpose of this study was to investigate the diversity, composition and structure of related microorganisms in patients with oral lichen planus (OLP). Methods　 The 16S rDNA gene sequencing of buccal or tongue mucosa samples from 12 reticular OLP patients, 12 erosive OLP patients and 8 healthy volunteers was carried out using sterile swabs to preliminarily explore the microbial dysbiosis of OLP patients. Results　 The relative abundance of bacteria of the OLP was significantly higher than that of the control group, including Eikenella, Alysiella, morococcus pneumoniae, Prevotella marshii, Leptotrichia buccalis c-1013-b and Granulicatella elegans. Among them, the relative abundance of Prevotella marshii was significantly higher in erosive OLP than in reticular OLP(P=0.0009). The relative abundance of Sediminibacterium and Streptococcus salivarius subsp. salivarius increased in the control group. Conclusions　The results showed that the characteristics of related microorganisms in the OLP were different from those in the control group. In order to determine the effective prevention and treatment strategies of OLP, further study on the diversity of OLP related microbial communities is of great importance.

Key words: oral lichen planus, microbiome, 16S rDNA gene, swab samples

中图分类号:

R781.59

王雪薇唐楠赵知白范媛. 口腔扁平苔藓黏膜病损区微生物群落分析[J]. 口腔医学, 2020, 40(2): 108-112.

参考文献 28

[1]	Sun, S.L., J.J. Liu, B. Zhong, et al., Topical calcineurin inhibitors in the treatment of oral lichen planus: a systematic review and meta-analysis. Br. J. Dermatol., 2019.
[2]	Mozaffari, H.R., E. Zavattaro, M. Saeedi, et al., Serum and salivary interleukin-4 levels in patients with oral lichen planus: A systematic review and meta-analysis. Oral Surg Oral Med Oral Pathol Oral Radiol, 2019. 128(2): 123-131.
[3]	Arweiler, N.B. and L. Netuschil, The Oral Microbiota. Adv. Exp. Med. Biol., 2016. 902: 45-60.
[4]	Belkaid, Y. and T.W. Hand, Role of the microbiota in immunity and inflammation. Cell, 2014. 157(1): 121-41.
[5]	de Paiva, C.S., D.B. Jones, M.E. Stern, et al., Altered Mucosal Microbiome Diversity and Disease Severity in Sj?gren Syndrome. Sci Rep, 2016. 6: 23561.
[6]	Corrêa, J.D., D.C. Calderaro, G.A. Ferreira, et al., Subgingival microbiota dysbiosis in systemic lupus erythematosus: association with periodontal status. Microbiome, 2017. 5(1): 34.
[7]	Shahi, S.K., S.N. Freedman, and A.K. Mangalam, Gut microbiome in multiple sclerosis: The players involved and the roles they play. Gut Microbes, 2017. 8(6): 607-615.
[8]	Khanna, S. and L.E. Raffals, The Microbiome in Crohn's Disease: Role in Pathogenesis and Role of Microbiome Replacement Therapies. Gastroenterol. Clin. North Am., 2017. 46(3): 481-492.
[9]	Katz-Agranov, N. and G. Zandman-Goddard, The microbiome and systemic lupus erythematosus. Immunol. Res., 2017. 65(2): 432-437.
[10]	He, Y., D. Gong, C. Shi, et al., Dysbiosis of oral buccal mucosa microbiota in patients with oral lichen planus. Oral Dis, 2017. 23(5): 674-682.
[11]	Kramer, I.R., R.B. Lucas, J.J. Pindborg, et al., Definition of leukoplakia and related lesions: an aid to studies on oral precancer. Oral Surg. Oral Med. Oral Pathol., 1978. 46(4): 518-39.
[12]	van der Meij, E.H. and I. van der Waal, Lack of clinicopathologic correlation in the diagnosis of oral lichen planus based on the presently available diagnostic criteria and suggestions for modifications. J. Oral Pathol. Med., 2003. 32(9): 507-12.
[13]	Caporaso, J.G., J. Kuczynski, J. Stombaugh, et al., QIIME allows analysis of high-throughput community sequencing data. Nat. Methods, 2010. 7(5): 335-6.
[14]	Schloss, P.D., S.L. Westcott, T. Ryabin, et al., Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl. Environ. Microbiol., 2009. 75(23): 7537-41.
[15]	Lozupone, C., M.E. Lladser, D. Knights, et al., UniFrac: an effective distance metric for microbial community comparison. Isme j, 2011. 5(2): 169-72.
[16]	Kim, M.H., M. Rho, J.P. Choi, et al., A Metagenomic Analysis Provides a Culture-Independent Pathogen Detection for Atopic Dermatitis. Allergy Asthma Immunol Res, 2017. 9(5): 453-461.
[17]	Downes, J., I. Sutcliffe, A.C. Tanner, et al., Prevotella marshii sp. nov. and Prevotella baroniae sp. nov., isolated from the human oral cavity. Int. J. Syst. Evol. Microbiol., 2005. 55(Pt 4): 1551-5.
[18]	Said, H.S., W. Suda, S. Nakagome, et al., Dysbiosis of salivary microbiota in inflammatory bowel disease and its association with oral immunological biomarkers. DNA Res., 2014. 21(1): 15-25.
[19]	Larsen, J.M., The immune response to Prevotella bacteria in chronic inflammatory disease. Immunology, 2017. 151(4): 363-374.
[20]	Canullo, L., D. Pe?arrocha-Oltra, U. Covani, et al., Microbiologic and Clinical Findings of Implants in Healthy Condition and with Peri-Implantitis. Int J Oral Maxillofac Implants. 30(4): 834-42.
[21]	Tricard, T., L. Bund, A. Alhefzi, et al., Eikenella corrodens bone and hip joint infection. A case report and literature review. Arch Pediatr, 2016. 23(11): 1146-1149.
[22]	Baris, O., T. Demir, and M. Gulluce, Investigation of Mineral forming bacterial isolates from supragingival calculus. Niger J Clin Pract, 2017. 20(12): 1571-1575.
[23]	Rai, N.P., P. Kumar, S.M. Mustafa, et al., Relation Between Periodontal Status and Pre-Cancerous Condition (Oral Lichen Planus): A Pilot Study. Adv Clin Exp Med. 25(4): 763-6.
[24]	Ohara-Nemoto, Y., K. Kishi, M. Satho, et al., Infective endocarditis caused by Granulicatella elegans originating in the oral cavity. J. Clin. Microbiol., 2005. 43(3): 1405-7.
[25]	Paju, S., J.M. Bernstein, E.M. Haase, et al., Molecular analysis of bacterial flora associated with chronically inflamed maxillary sinuses. J. Med. Microbiol., 2003. 52(Pt 7): 591-7.
[26]	Larsen, N., F.K. Vogensen, F.W. van den Berg, et al., Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. PLoS ONE, 2010. 5(2): e9085.
[27]	Zheng, H., H. Liang, Y. Wang, et al., Altered Gut Microbiota Composition Associated with Eczema in Infants. PLoS ONE, 2016. 11(11): e0166026.
[28]	Gao, L., T. Xu, G. Huang, et al., Oral microbiomes: more and more importance in oral cavity and whole body. Protein Cell, 2018. 9(5): 488-500.