正畸骨改建中Hedgehog通路下游信号的表达变化

摘要/Abstract

摘要： [摘要]目的 Hedgehog信号通路在骨发育中有重要作用。本研究探讨Hedgehog通路下游信号在正畸骨改建中的表达变化。方法建立小鼠上颌正畸模型，在加力后0天、7、14天进行观察。micro-CT测量牙齿移动距离；HE染色和Trap染色观察正畸骨改建和破骨细胞生成状况；免疫荧光染色观察骨改建中Gli1阳性细胞表达变化；Real time-PCR检测Hedgehog信号通路分子表达水平及纤毛相关转运蛋白表达变化。结果正畸加力牙移动距离随时间逐渐增加；HE染色和Trap染色示加力后牙槽骨改建明显且破骨细胞生成活跃；免疫荧光染色示Gli1阳性细胞在牙槽骨压力侧聚集表达，在加力第7天达到峰值（P<0.05）；Hedgehog通路下游信号关键分子Shh，Smo，Gli1表达水平高于正畸前（P<0.05）；各时间点纤毛相关转运蛋白表达水平变化有统计学意义（P<0.05）。结论 Hedgehog信号通路下游分子和纤毛相关转运蛋白参与正畸骨改建过程。

关键词: [关键词]正畸骨改建, 破骨细胞, Hedgehog信号通路, 纤毛转运蛋白

Abstract: Abstract: Objective Hedgehog pathway play a vital role in bone development. This study investigated the expression of Hedgehog pathway signals in orthodontic bone remodeling. Methods We established the mice orthodontic model and observed on day 0, day 7, day 14 after orthodontic treatment. Tooth movement distance measured by micro-CT. HE&TRAP staining were used to investigated the orthodontic bone remodeling and osteoclastogenesis. Immunofluorescence staining was used for the expression of Gli1 positive cells in bone remodeling. Hedgehog pathway molecules mRNA expression levels and the expression changes of Intraflagellar transport(IFT) protein detected by Real time-PCR. Results The orthodontic tooth movement distance gradually increased. HE&TRAP staining showed the alveolar bone remodeling is obviously and osteoclastogenesis actively after treatment. Immunofluorescence showed a significant increased in the expression of Gli1 positive cells, which was mainly expressed in the alveolar bone pressure side, and peaked on the 7th day of orthodontic force(P<0.05). The mRNA expression of Hedgehog pathway vital molecules Shh, Smo, Gli were higher than control group(P<0.05). The changes of intraflagellar transport protein mRNA expression levels at each time point were statistically significant (P<0.05). Conclusion The Hedgehog pathway signals and Intraflagellar transport (IFT) protein participate in the orthodontic alveolar bone remolding.

Key words: Key words: orthodontic bone remodeling, osteoclast, hedgehog pathway, intraflagellar transport (IFT) protein

中图分类号:

R783.5

荣崧汐周梦琪孙瑶. 正畸骨改建中Hedgehog通路下游信号的表达变化[J]. 口腔医学, 2020, 40(4): 293-298.

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