三碘甲腺原氨酸对根尖牙乳头干细胞成牙/骨向分化的影响及其机制的初步探究

›› 2020, Vol. 40 ›› Issue (6): 500-505.

三碘甲腺原氨酸对根尖牙乳头干细胞成牙/骨向分化的影响及其机制的初步探究

夏翊博¹,¹,于金华²

1. 南京医科大学
2. 南京医科大学附属口腔医院

收稿日期:2020-02-06 修回日期:2020-03-17 出版日期:2020-06-28 发布日期:2020-06-28
通讯作者: 于金华 E-mail:yujinhua@njmu.edu.cn
基金资助:
国家自然科学基金面上项目;江苏省“科教强卫”医学重点人才项目;江苏省自然科学基金

Effect of Triiodothyronine on the odonto/ostegenic differentiation of stem cells from apical papilla and the preliminary study of its mechanism

Yi-Bo Xia¹,¹,

Received:2020-02-06 Revised:2020-03-17 Online:2020-06-28 Published:2020-06-28

摘要/Abstract

摘要： ［摘要］　目的　探讨三碘甲腺原氨酸（3,5,3'-triiodothyronine?T3）对根尖牙乳头干细胞(stem cell from apical papilla, SCAPs)成骨向分化的影响。方法　酶消化法分离培养SCAPs。制备含不同浓度 T3的条件培养基，培养5d，检测各组碱性磷酸酶表达，选取差异最显著浓度的作为实验浓度。CCK-8法检测增殖能力；real-time RT-PCR、Western Blot检测牙/骨向分化相关基因、蛋白的表达情况。Western blot检测MAPK通路相关蛋白表达情况。结果　T3实验组对增殖能力没有显著影响并促进SCAPs牙、骨向分化，并激活ERK通路；U0126抑制牙、骨向分化相关蛋白表达。结论　T3促进SCAPs牙、骨向分化，这个过程与ERK通路有密切关联。

关键词: 三碘甲腺原氨酸, 根尖牙乳头干细胞, 成牙/骨向分化

Abstract: Abstract：　Objective The aim of this study is to examined the effect of T3 on the odonto/ostegenic differentiation of SCAPs. Methods After treated with T3, the ALP was measured to select the optimum concentration. The CCK‐8 were taken to distinguish the proliferation ability between the T3-treated group and the control group. To evaluate the odonto/ostegenic differentiation potential of the T3-treated SCAPs, RT-PCR, western blot, Alkaline Phosphatase Staining was performed. The MAPK signal pathway was detected via western blot. Results 10-9 mol/L T3-treated group expressed the highest ALP among all groups (p < 0.01) and it was chosen as the optimal concentration. CCK-8 demonstrated that T3 had no significant effect on the proliferation of SCAPs (p > 0.05). Moreover, results of real-time RT-PCR, western blot, showed that 10-9 mol/L T3 increased odonto/ostegenic differentiation of SCAPs. Besides, ERK signal pathway was activated at 15 min after treated with T3. The odonto/ostegenic related protein was suppressed by U0126. Conclusions 10-9 mol/L T3 promote the osteogenic differentiation of SCAPs and this process may be closely realted to the ERK signal pathway.

Key words: T3, SCAPs, odonto/ostegenic differentiation

夏翊博于金华. 三碘甲腺原氨酸对根尖牙乳头干细胞成牙/骨向分化的影响及其机制的初步探究[J]. 口腔医学, 2020, 40(6): 500-505.

Yi-Bo Xia. Effect of Triiodothyronine on the odonto/ostegenic differentiation of stem cells from apical papilla and the preliminary study of its mechanism[J]. Stomatology, 2020, 40(6): 500-505.

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