一种国产菌斑产酸龋活性培养基与标准试剂一致性检测实验的有效性检测

›› 2020, Vol. 40 ›› Issue (7): 602-605.

一种国产菌斑产酸龋活性培养基与标准试剂一致性检测实验的有效性检测

VIVIEN FENG¹,陈曦¹,张羽¹,吴日玥²,曹桂芝³

1. 上海交通大学医学院附属第九人民医院
2. 安徽医科大学第一附属医院
3. 上海交通大学附属第九人民口腔医学院

收稿日期:2019-12-23 修回日期:2020-02-17 出版日期:2020-07-28 发布日期:2020-07-20
通讯作者: 陈曦 E-mail:cherry_cxnet@hotmail.com
基金资助:
国家自然科学基金项目

The practicability test of consistency between domestic caries active medium and standard caries active medium by acid production.

Received:2019-12-23 Revised:2020-02-17 Online:2020-07-28 Published:2020-07-20

摘要/Abstract

摘要： ［摘要］　目的　比较一种国产龋活性培养基和日本Cariostat龋活性培养基在菌斑产酸性方面的一致性、敏感性和准确性，借此评估国产龋活性培养基的临床可行性。方法　对变异链球菌、血链球菌、唾液链球菌、远缘链球菌、嗜酸乳杆菌、牙龈卟啉单胞菌、具核梭杆菌、粘性放线菌8种测试菌种4个浓度梯度以及空白对照样本，采用两种培养基分别培养48小时，相同条件下，采用单盲法分别由3位检查者进行读数，并采用SPSS19.0统计软件对结果进行统计分析。结果　两种培养基Kappa值大于0.8，国产试剂盒相对于日本试剂盒的敏感度为94.7%，特异度为85.6%，准确率为90.4%。结论　本次试验选择的国产菌斑产酸检测技术可能是一种非常有希望的替代品，龋病为多因素致病疾病，仍需要寻求更多高准确度、高性价比、易获取的实验产品

关键词: 龋病, 龋活性测试, Cariostat, 国产培养基

Abstract: Abstract：　Objective: To compare the consistency, sensitivity and accuracy between a caries activity test from China and Cariostat test from Japan and to evaluate the clinical feasibility of the former one. Material and methods: Eight species of oral bacteria, including S. mutans, S. sanguinis, S. salivarius, S. sobrinus, L. acidophilus, P. gingivalis, F. nucleatum and A. viscosus, were diluted in four concentrations and cultured in these two mediums separately for 48 hours. Blank negative control was also applied. Three blind tester reported the results under same condition, which were then analyzed by SPSS 19.0. Results: The kappa value between these two tests was over 0.8. The sensibility of the test kit was 94.7%, specificity was 85.6% and accuracy was 90.4%. Conclusion: The acid-production detection technique from oral plaque selected in this study is a promising substitute for Cariostat test. Products with better accuracy, higher cost performance and easier accessibility are still needed.

Key words: caries, caries activity test, Cariostat, domestic medium

中图分类号:

R781.1

VIVIEN FENG 陈曦张羽吴日玥曹桂芝. 一种国产菌斑产酸龋活性培养基与标准试剂一致性检测实验的有效性检测[J]. 口腔医学, 2020, 40(7): 602-605.

参考文献 21

[1]	王兴，冯希平，李志新.第四次全国口腔健康流行病学[M] . 第一版.北京:人民卫生出版社,2018: 40-50
[2]	毛培芬,汪俊.龋患风险预测的方法[J].口腔材料器械杂志,2009,18(02):89-91.
[3]	Rita S. Villena,Eraldo Pesaressi,Jo E. Frencken. Reducing carious lesions during the first 4 years of life[J]. The Journal of the American Dental Association,2019,150(12)．
[4]	台保军.第四次全国口腔健康流行病学调查知信行结果报告[C].中华口腔医学会口腔预防医学专业委员会.2018年中华口腔医学会第十八次口腔预防医学学术年会论文汇编.中华口腔医学会口腔预防医学专业委员会:中华口腔医学会,2018:15
[5]	石四箴,药师寺仁,町田幸雄.龋病活跃性试验的应用研究[J].国外医学口腔医学分册,1997(04):204-207.
[6]	李健,刘璐.应用Cariogram系统评估龋病风险的研究进展[J].中国医科大学学报,2018,47(05):461-463.
[7]	樊明文．牙体牙髓病学[Ｍ]第４版.北京：人民卫生出版社，2012:3
[8]	张举之.口腔内科学[M].第3 版.北京:人民卫生出版社, 1997.
[9]	袁峻伟,玄松玉,王珏,关雪琳.北京市海淀区3岁儿童龋病患病状况及龋病活跃性检测的研究[J].北京口腔医学,2017,25(03):166-168
[10]	Ramamurthy PH, Swamy H S, Bennete F,et al. Relationship between severe-early childhood caries, salivary mutans streptococci, and lactobacilli in preschool children of low socioeconomic status in Bengaluru city[J]. Journal of the Indian Society of Pedodontics & Preventive Dentistry, 2014;32(1):44-47.
[11]	van Ruyven FO , Lingstr?m P, van H out e J, et al.Relationship among mutans strept ococci , “ low-pH” bacteria, and lodophilic polysaccharide-producing bacteria in dent al plaque and early enamel caries in humans[J] Journal of Dental Research, 2000, 79:778-784 .
[12]	Rather Shabeer A,Sharma Sukesh C,Mahmood Akhtar. pH dependent effects of sodium ions on dextransucrase activity in Streptococcus mutans[J]. Biochemistry and biophysics reports,2019,20.
[13]	刘芸,张强,杨秀巧,孟玉生.唾液变形链球菌感染水平与乳牙龋病现状及发展的相关性分析[J].口腔生物医学,2019,10(03):131-134
[14]	赵岩,靳趁心.龋病高危人群预测和个体化预防措施的研究进展[J].全科口腔医学电子杂志,2019,6(09):35-36+49.
[15]	许世梃,石四箴,梁勤.两种龋病活跃性检测法的比较研究[J].上海医学,2010,33(07):663-666
[16]	兰育林,冯嘉健,陈培培.低龄儿童龋危险因素分析[J].深圳中西医结合杂志,2019,29(11):91-92.
[17]	梁勤,石四箴.幼儿龋病活跃性的三年追踪研究——CRT bacteria的临床应用[J].同济大学学报(医学版),2014,35(06):136.
[18]	刘梁婧.6-17岁青少年龋活跃性之研究分析[J].全科口腔医学电子杂志,2019,6(29):156.
[19]	梁勤,石四箴.CRT~@ bacteria检测幼儿龋病活跃性的研究[J].同济大学学报(医学版),2015,36(01):105-109.
[20]	林晓华,王文红,邹晓璇,苗江霞,崔秀丽.北京市0～2岁低龄儿童龋与龋活跃性Cariostat值的相关关系[J].中国儿童保健杂志,2019,27(02):222-225
[21]	刘怡然,张年保,蔡晨星,刘茜.婴幼儿龋口腔致龋菌活性的研究[J].口腔生物医学,2018,9(01):33-35.