雌激素联合机械张力调控人牙周膜成纤维细胞成骨分化及机制研究

›› 2021, Vol. 41 ›› Issue (1): 6-11.

雌激素联合机械张力调控人牙周膜成纤维细胞成骨分化及机制研究

赵玺¹,李向宇²,丁锐²,赵浩然²,米丛波³,吴晓琴⁴

1. 乌鲁木齐市口腔医院
2. 乌鲁木齐市口腔医院正畸科
3. 新疆医科大学第一附属医院口腔正畸科
4. 新疆医科大学基础医学院病理生理学科

收稿日期:2020-05-26 修回日期:2020-07-21 出版日期:2021-01-28 发布日期:2021-01-28
通讯作者: 赵玺 E-mail:mignon98@163.com
基金资助:
新疆维吾尔自治区自然科学基金

Estrogen combined with mechanical tension to regulate osteogenic differentiation of human periodontal ligament fibroblasts and its mechanism

Received:2020-05-26 Revised:2020-07-21 Online:2021-01-28 Published:2021-01-28

摘要/Abstract

摘要： 目的　探究雌激素联合机械张力对人牙周膜成纤维细胞成骨分化的影响及机制。方法　分离hPDLF细胞并鉴定，将hPDLF细胞分为对照组、17β-雌二醇组、机械张力组、17β-雌二醇联合机械张力组；按细胞给药和加力方案处理后，检测细胞增殖能力，茜素红染色检测hPDLF细胞成骨分化，qRT-PCR检测成骨基因mRNA的表达，Western blot检测成骨蛋白、雌激素受体表达及Akt信号通路的激活。结果　鉴定所提取到的hPDLF细胞符合其特征；17β-雌二醇组和机械张力组细胞增殖能力、茜素红染色OD值均显著高于对照组；雌激素受体ER-β、成骨基因RUNX2、OCN、OSX、OPN表达，Akt磷酸化水平均显著高于对照组；雌激素联合机械张力细胞增殖能力、茜素红染色OD值显著高于17β-雌二醇和机械张力组单独作用组，雌激素受体ER-β、成骨基因RUNX2、OCN、OSX、OPN表达，Akt磷酸化水平均显著高于17β-雌二醇和机械张力组单独作用组。结论　雌激素联合机械张力能够促进hPDLF细胞成骨分化，并且促进雌激素受体和成骨基因的表达，以及Akt信号通路的激活。

关键词: 雌激素联合机械张力, 成骨分化, 机制

Abstract: Objective　To investigate the effect and mechanism of estrogen combined with mechanical tension on human periodontal ligament fibroblasts. Methods　hPDLF cells were sorted and identified by magnetic beads. The hPDLF cells were divided into the control group, 17β-estradiol group, mechanical tension group and 17β-estradiol combined with mechanical tension group. Cell proliferation was measured; alizarin red staining was used to detect osteogenic differentiation of hPDLF cells. qRT-PCR was used to detect osteogenic gene mRNA expression, and Western blot was used to detect osteogenic protein, estrogen receptor expression, and activation of Akt signaling pathway. Results　It was identified that the extracted hPDLF cells conformed to its characteristics. The cell proliferation ability and OD-value of alizarin red staining in the 17β-estradiol and mechanical tension groups were significantly higher than those in the control group. The estrogen receptor ER-β and osteogenic genes RUNX2, OCN, OSX, OPN expression, Akt phosphorylation level were significantly higher than those of control group. Estrogen combined with mechanical tension cell proliferation ability, alizarin red staining OD value was significantly higher than the 17β-estradiol and mechanical tension group. The expression of estrogen receptor ER-β, osteogenic genes RUNX2, OCN, OSX, OPN, Akt phosphorylation level were significantly higher than those of 17β-estradiol and mechanical tension group. Conclusion　Estrogen combined with mechanical tension can promote osteogenic differentiation of hPDLF cells, and promote the expression of estrogen receptors and osteogenic genes, as well as the activation of Akt signaling pathway.

Key words: estrogen combined with mechanical tension, osteogenic differentiation, mechanism

中图分类号:

R349.5

赵玺李向宇丁锐赵浩然米丛波吴晓琴. 雌激素联合机械张力调控人牙周膜成纤维细胞成骨分化及机制研究[J]. 口腔医学, 2021, 41(1): 6-11.

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