Prx1+骨骼干细胞的研究进展

›› 2021, Vol. 41 ›› Issue (1): 83-86.

Prx1+骨骼干细胞的研究进展

曾毓璐¹,唐国华²

1. 上海交通大学医学院附属第九人民医院
2. 上海交通大学口腔医学院附属第九人民医院

收稿日期:2020-03-03 修回日期:2020-04-08 出版日期:2021-01-28 发布日期:2021-01-28
通讯作者: 唐国华 E-mail:drtanggh@163.com
基金资助:
上海市自然科学基金

Research progress of rx1+ skeletal stem cells

Received:2020-03-03 Revised:2020-04-08 Online:2021-01-28 Published:2021-01-28
Supported by:
Natural Science Foundation of Shanghai

摘要/Abstract

摘要： 配对相关同源基因1(paired related homeobox 1, Prx1/Prrx1/MHox）在胚胎肢芽和颅面发育中高表达，成体中的Prx1+细胞分布在长骨的骨膜、骨髓和颅骨的骨缝中，表现出间充质干细胞（mesenchymal stem cells，MSCs）或骨骼干细胞（skeletal stem cells，SSCs）的特征。近期的研究显示，表达Prx1基因的骨骼干细胞（Prx1+ skeletal stem cells, Prx1+SSCs）参与了骨骼的创伤愈合和对机械应力的应答反应。同时，牙周膜中Prx1+细胞在牙周组织正常发育和缺损修复中发挥的作用也受到关注。该文回顾Prx1基因和Prx1+细胞的特征和功能，总结Prx1+SSCs对骨骼改建或修复的作用，有助于SSCs在调控颅面骨生长、促进牙槽骨再生中的深入研究。

关键词: 骨骼干细胞, Prx1, 颅面部, 骨改建, 机械力

Abstract: The paired related homeobox gene Prx1 is highly expressed during embryonic lib formation and craniofacial development. Postnatal Prx1+cells located in periosteum, bone marrow or calvarial sutures display the characteristics of mesenchymal stem cells(MSCs) or skeletal stem cells(SSCs). Recent research has shown that Prx1+SSCs participate in fracture healing and respond to mechanical forces in bone. At the same time, Prx1+cells in the periodontal ligament are noticed for its contribution to the normal development and defect repair of periodontal tissues. This paper reviews the characteristics and function of Prx1 gene and Prx1+cells and gives a summary of the role Prx1+SSCs play in bone remodeling and repair, which is helpful for the further studies of SSCs in regulating orchestrate craniofacial growth and promoting the regeneration of alveolar bones.

Key words: skeletal stem cells, Prx1, craniofacial, bone remodeling, mechanical force

中图分类号:

R349.6

曾毓璐唐国华. Prx1+骨骼干细胞的研究进展[J]. 口腔医学, 2021, 41(1): 83-86.

参考文献

1. Bianco P, Robey PG. Skeletal stem cells[J].Development. 2015,142(6):1023.
2. Ambrosi TH, Longaker MT, Chan CKF. A Revised Perspective of Skeletal Stem Cell Biology[J].Frontiers in cell and developmental biology. 2019,7:189.
3. Doro DH, Grigoriadis AE, Liu KJ. Calvarial Suture-Derived Stem Cells and Their Contribution to Cranial Bone Repair[J].Front Physiol. 2017,8:956.
4. ten Berge D, Brouwer A, Korving J, et al. Prx1 and Prx2 in skeletogenesis: roles in the craniofacial region, inner ear and limbs[J].Development. 1998,125(19):3831-42.
5. Martin JF, Bradley A, Olson EN. The paired-like homeo box gene MHox is required for early events of skeletogenesis in multiple lineages[J].Genes & development. 1995,9(10):1237-49.
6. Lu MF, Cheng HT, Kern MJ, et al. prx-1 functions cooperatively with another paired-related homeobox gene, prx-2, to maintain cell fates within the craniofacial mesenchyme[J].Development. 1999,126(3):495-504.
7. Mitchell JM, Hicklin DM, Doughty PM, et al. The Prx1 homeobox gene is critical for molar tooth morphogenesis[J].Journal of dental research. 2006,85(10):888-93.
8. Balic A, Adams D, Mina M. Prx1 and Prx2 cooperatively regulate the morphogenesis of the medial region of the mandibular process[J].Developmental dynamics : an official publication of the American Association of Anatomists. 2009,238(10):2599-613.
9. Dasouki M, Andrews B, Parimi P, et al. Recurrent agnathia-otocephaly caused by DNA replication slippage in PRRX1[J].American journal of medical genetics Part A. 2013,161a(4):803-8.
10. Guo L, Huang W, Chen B, et al. gga-mir-133a-3p Regulates Myoblasts Proliferation and Differentiation by Targeting PRRX1[J].Frontiers in genetics. 2018,9:577.
11. Du B, Cawthorn WP, Su A, et al. The transcription factor paired-related homeobox 1 (Prrx1) inhibits adipogenesis by activating transforming growth factor-beta (TGFbeta) signaling[J].The Journal of biological chemistry. 2013,288(5):3036-47.
12. Lu X, Beck GR, Jr., Gilbert LC, et al. Identification of the homeobox protein Prx1 (MHox, Prrx-1) as a regulator of osterix expression and mediator of tumor necrosis factor alpha action in osteoblast differentiation[J].J Bone Miner Res. 2011,26(1):209-19.
13. Wang J, Saraswat D, Sinha AK, et al. Paired Related Homeobox Protein 1 Regulates Quiescence in Human Oligodendrocyte Progenitors[J].Cell reports. 2018,25(12):3435-50.e6.
14. Shimozaki K, Clemenson GD, Jr., Gage FH. Paired related homeobox protein 1 is a regulator of stemness in adult neural stem/progenitor cells[J].The Journal of neuroscience : the official journal of the Society for Neuroscience. 2013,33(9):4066-75.
15. Logan M, Martin JF, Nagy A, et al. Expression of Cre Recombinase in the developing mouse limb bud driven by a Prxl enhancer[J].Genesis (New York, NY : 2000). 2002,33(2):77-80.
16. Greenbaum A, Hsu YM, Day RB, et al. CXCL12 in early mesenchymal progenitors is required for haematopoietic stem-cell maintenance[J].Nature. 2013,495(7440):227-30.
17. Duchamp de Lageneste O, Julien A, Abou-Khalil R, et al. Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin[J].Nat Commun. 2018,9(1):773.
18. Kawanami A, Matsushita T, Chan YY, et al. Mice expressing GFP and CreER in osteochondro progenitor cells in the periosteum[J].Biochemical and biophysical research communications. 2009,386(3):477-82.
19. Ouyang Z, Chen Z, Ishikawa M, et al. Prx1 and 3.2kb Col1a1 promoters target distinct bone cell populations in transgenic mice[J].Bone. 2014,58(1):136-45.
20. Moore ER, Zhu YX, Ryu HS, et al. Periosteal progenitors contribute to load-induced bone formation in adult mice and require primary cilia to sense mechanical stimulation[J].Stem cell research & therapy. 2018,9(1):190.
21. Wang Y, Chen L, Kang M, et al. The Fracture Callus Is Formed by Progenitors of Different Skeletal Origins in a Site-Specific Manner[J].JBMR plus. 2019,3(9):e10193.
22. Cabahug-Zuckerman P, Liu C, Cai C, et al. Site-Specific Load-Induced Expansion of Sca-1(+)Prrx1(+) and Sca-1(-)Prrx1(+) Cells in Adult Mouse Long Bone Is Attenuated With Age[J].JBMR plus. 2019,3(9):e10199.
23. Liu C, Cabahug-Zuckerman P, Stubbs C, et al. Mechanical Loading Promotes the Expansion of Primitive Osteoprogenitors and Organizes Matrix and Vascular Morphology in Long Bone Defects[J].J Bone Miner Res. 2019,34(5):896-910.
24. Moore ER, Chen JC, Jacobs CR. Prx1-Expressing Progenitor Primary Cilia Mediate Bone Formation in response to Mechanical Loading in Mice[J].Stem cells international. 2019,2019:3094154.
25. Wilk K, Yeh S-CA, Mortensen LJ, et al. Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration[J].Stem cell reports. 2017,8(4):933-46.
26. Yeh SA, Wilk K, Lin CP, et al. In Vivo 3D Histomorphometry Quantifies Bone Apposition and Skeletal Progenitor Cell Differentiation[J].Scientific reports. 2018,8(1):5580.
27. Bassir SH, Garakani S, Wilk K, et al. Prx1 Expressing Cells Are Required for Periodontal Regeneration of the Mouse Incisor[J].Front Physiol. 2019,10:591.

[1]	蔡昀唐燚康非吾. 下颌升支截骨去血供后牙槽骨内氧水平变化与骨改建的变化研究[J]. , 2020, 40(10): 869-873.
[2]	张瑞林孙瑶. 正畸牙槽骨改建中RANKL/OPG表达动态变化特征及牙周组织细胞凋亡观察[J]. , 2019, 39(6): 488-493.
[3]	杨迷芳马兰严冬琳蒋玲玲. 麦考酚酸对斑马鱼胚胎颅面部发育的影响[J]. , 2019, 39(2): 103-107.
[4]	薛敏郑海英封伟刘国惠. 不同平台转移距离对种植体周围骨改建的影响[J]. , 2018, 38(4): 329-332.
[5]	纪映辰康非吾. 手术先行治疗牙颌面畸形的研究进展[J]. , 2017, 37(8): 742-745.
[6]	杨鑫铭柯杰. 上下颌联合扩弓对牙槽骨改建影响因素的研究现状[J]. , 2017, 37(7): 660-663.
[7]	莫士诚赵春洋王林. 应用功能矫治器下颌前伸髁状突改建的研究进展[J]. , 2014, 34(8): 627-630.
[8]	巫云霞;王林;张卫兵;马俊青;吴蓉宁;张文健. 扩张早期大鼠腭中缝细胞凋亡现象的观察[J]. , 2007, 27(4): 175-177.
[9]	郑茜聪;刘丽. 骨保护素及其配体在口腔领域的研究进展[J]. , 2007, 27(2): 104-106.