新型饵激光与超声对根管荡洗作用的对比研究

摘要/Abstract

摘要： 目的比较Er:YAG激光中的SWEEPS 模式（shock wave enhanced emission photoacoustic streaming，SWEEPS）和超声非切割(passive ultrasonic irrigation，PUI) 两种荡洗方式对根管内粪肠球菌（Enterococcus faecalis，E. faecalis）生物膜的清除作用。方法选择人新鲜拔除的单根前磨牙共250颗，去除管壁玷污层，高温高压灭菌，建立E.faecalis感染模型。随机分为6组（n=40），SWEEPS-Er:YAG激光联合3%NaClO，SWEEPS-Er:YAG 激光联合0.9%生理盐水,PUI联合3%NaClO，PUI联合0.9%生理盐水作为实验组，3%NaClO和0.9%生理盐水组作为对照组。扫描电镜(scanning electron microscopy,SEM)定性观察两种方式细菌清除作用,平板细菌计数法定量检测两种方式细菌减少率，非参数检验进行统计学分析。结果与荡洗前比，SWEEPS+NaClO组、SWEEPS+生理盐水、PUI+NaClO组、PUI+生理盐水组、3%NaClO组在荡洗后E. faecalis菌量显著降低（P＜0.05），组间比较有统计学差异（P＜0.05），细菌减少率SWEEPS+NaClO组>SWEEPS+生理盐水组>PUI+NaClO组>PUI+生理盐水组>NaClO组>生理盐水组。结论 SWEEPS-Er:YAG激光荡洗对于根管内粪肠球菌生物膜的清除作用明显优于超声非切割荡洗。

关键词: SWEEPS-Er:YAG激光, 超声非切割荡洗, 根管荡洗, 粪肠球菌生物膜

Abstract: Abstract：　Objective　 This study was designed to compare the clearning efficiency between shock wave enhanced emission photoacoustic streaming (SWEEPS) and Passive Ultrasonic Irrigation（PUI）Methods　250 single premolar from freshly extracted human were collected, the smear layers were removed. After sterilization, the model of E.faecalis infection was established in vitro. Samples were randomly divided into 6 groups(n=40) respectively, SWEEPS-Er:YAG laser with 3% NaOCl, SWEEPS-Er:YAG laser with 0.9% normal saline, PUI with 3% NaOCl , PUI with 0.9% normal saline as the experimental group, 3% NaOCl group and 0.9% normal saline group were the control groups. After irrigation, the bacterial clearing effectiveness were qualitatively observed through a scanning electron microscope, and the rate of bacterial reduction was quantitatively determined by the plate bacterial counting method, and all data were statistically analyzed by a Mann-Whitney U test. Results　 The amount of E.faecalis was significantly decreased in SWEEPS+NaOCl group, SWEEPS+NS group, PUI+NaOCl group, PUI+NS group and 3% NaOCl group (P < 0.05). The difference between the each two groups was statistically significant (P < 0.05). The efficiency of bacterial reduction was SWEEPS+NaOCl group > SWEEPS+NS group > PUI+NaOCl group > PUI+NS group > NaOCl group > NS group. Conclusions　 The clearning efficiency of SWEEPS-Er:YAG irrigation was significantly better than that of Passive Ultrasonic Irrigation.

Key words: SWEEPS-Er:YAG laser, Passive Ultrasonic Irrigation, root canal Irrigation, Enterococcus faecalis biofilm

中图分类号:

R781.05

王晓娜石晶. 新型饵激光与超声对根管荡洗作用的对比研究[J]. 口腔医学, 2021, 41(4): 328-332.

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