DHA对人牙龈成纤维细胞生物学活性及炎症因子表达的作用

›› 2021, Vol. 41 ›› Issue (5): 385-390.

• 基础研究 • 下一篇

DHA对人牙龈成纤维细胞生物学活性及炎症因子表达的作用

孙梦君¹,周可聪²,夏一如²,谢玉峰²,束蓉¹

1. 上海交通大学医学院附属第九人民医院
2. 上海交通大学医学院附属第九人民医院牙周科

收稿日期:2020-12-14 修回日期:2021-02-08 出版日期:2021-05-28 发布日期:2021-05-21
通讯作者: 束蓉 E-mail:shurong123@hotmail.com
基金资助:
国家自然科学基金重大项目;口腔医学重点核心口腔颌面组织再生与功能修复创新团队;上海交通大学医学院附属第九人民医院交叉基金;九院基础研究助推计划

Effects of DHA on biological activity and inflammatory factor expression of human gingival fibroblasts

Received:2020-12-14 Revised:2021-02-08 Online:2021-05-28 Published:2021-05-21

摘要/Abstract

摘要： 目的探索二十二碳六烯酸（docosahexaenoic acid，DHA）对人牙龈成纤维细胞（human gingival fibroblasts，HGFs）生物学活性及炎症因子表达的作用。方法分别采用活死细胞染色法、荧光染色法、流式细胞术观察DHA对细胞活性、细胞形态、细胞周期的影响；DHA预处理HGFs后，利用牙龈卟啉单胞菌（Porphyromonas gingivalis，P.gingivalis）脂多糖（lipopolysaccharides，LPS）或热灭活P.gingivalis刺激细胞，定量PCR和ELISA观察白介素-6（interleukin-6，IL-6）、IL-8、IL-1β基因和蛋白表达的变化；通过定量PCR和Western blot检测DHA对血红素氧合酶-1（heme oxygenase-1，HO-1）基因和蛋白表达的作用。结果 200 μmol/L DHA可降低HGFs的细胞活性，但100 μmol/L DHA不影响细胞活性及形态，对细胞周期也无明显影响（P＞0.05）；DHA可抑制P.gingivalis LPS或热灭活P.gingivalis诱导HGFs表达的IL-6、IL-8、IL-1β mRNA以及IL-6、IL-8蛋白（P＜0.05），并可显著上调HGFs表达的HO-1 mRNA及蛋白（P＜0.05）。结论 DHA可显著抑制HGFs的炎症因子表达，并且不影响其生物学活性，其抗炎作用可能与HO-1上调相关。

关键词: 二十二碳六烯酸, 牙龈成纤维细胞, 生物学活性, 炎症反应, 血红素氧合酶-1

Abstract: ObjectiveTo explore the effects of docosahexaenoic acid (DHA) on the biological activity and inflammatory response of human gingival fibroblasts (HGFs).MethodsThe effects of DHA on cell viability, cell morphology and cell cycle were observed by living and dead cell staining, fluorescent staining and flow cytometry, respectively. After pretreated with DHA, HGFs were stimulated with lipopolysaccharide (LPS) from Porphyromonasgingivalis (P. gingivalis) or with heat- inactivated P. gingivalis. Then the gene and protein expression of interleukin-6 (IL-6), IL-8 and IL-1β was detected by quantitative PCR and ELISA, respectively. The effect of DHA on heme oxygenase-1 (HO-1) gene and protein expression was also observed by quantitative PCR and western blot, respectively. Results200 μmol/L DHA reduced the cell activity of HGFs, but 100 μmol/L DHA had no effect on cell viability and morphology, and had no significant effect on cell cycle (P>0.05); DHA inhibited the expression of IL-6, IL-8, IL-1β mRNA and IL-6, IL-8 protein induced by P. gingivalis LPS or heat- inactivated P. gingivalis in a concentration-dependent manner (P<0.05); DHA significantly induced HO-1 mRNA and protein expression (P<0.05).ConclusionDHA significantly inhibited the inflammatory response of HGFs without affecting the biological activity. The anti-inflammatory effect may be related to the induction of HO-1.

Key words: docosahexaenoic acid, human gingival fibroblasts, biological activity, inflammatory response, heme oxygenase-1

中图分类号:

R781.4

孙梦君周可聪夏一如谢玉峰束蓉. DHA对人牙龈成纤维细胞生物学活性及炎症因子表达的作用[J]. 口腔医学, 2021, 41(5): 385-390.

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DHA对人牙龈成纤维细胞生物学活性及炎症因子表达的作用

Effects of DHA on biological activity and inflammatory factor expression of human gingival fibroblasts

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