赤芍的抑菌作用及其防治慢性牙周炎的机制研究

摘要/Abstract

摘要： 目的研究赤芍提取物对牙龈卟啉单胞菌（Porphyromonas gingivalis，P.g）、具核梭杆菌（Fusobacterium nucleatum，F.n）和伴放线聚集杆菌（Actinobacillus Actinomycetes，A.a）的抑菌作用，并通过网络药理学方法分析赤芍防治慢性牙周炎（chronic periodontitis，CP）的潜在分子机制和药理活性。方法采用倍比稀释法测定赤芍提取物对3种牙周致病菌（P.g、F.n、A.a）的最低抑菌浓度（minimum inhibitory concentration，MIC）及最低杀菌浓度（minimal bactericidal concentration，MBC）。借助TCMSP数据库获取赤芍化学成分及作用靶点，通过Genecards等数据库获取CP疾病靶点，韦恩分析取交集。利用Cytoscape 3.7.2软件构建赤芍防治CP的“成分-靶点-疾病”网络，采用STRING数据库构建蛋白互作网络（Protein protein interaction network，PPI network），并进行基因本体（Gene Ontology，GO）和京都基因和基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）富集分析。结果赤芍提取物对P.g、F.n、A.a的MIC分别为2.0、4.0、4.0 g/L，MBC分别为4.0、8.0、8.0 g/L。分析获得赤芍活性成分29个，CP疾病靶点2 132个，交集靶点89个。赤芍治疗CP的主要活性成分有黄芩苷、鞣花酸等，关键靶点包括白细胞介素-6（Interleukin-6，IL-6）等。GO富集分析得到蛋白质异构化活性等126个GO条目，KEGG富集分析得到丝裂原活化蛋白激酶（Mitogen-activated protein kinase，MAPK）等110条通路。结论赤芍提取物对3种牙周致病菌均有不同程度的抑菌作用，初步阐述了赤芍的主要成分、作用靶点和相关通路在防治CP中具有重要的研究价值，为进一步深入临床研究提供实验依据和理论支持。

关键词: 赤芍, 慢性牙周炎, 抑菌活性, 网络药理学, 作用机制

Abstract: 　Objective　 Objective to study the effect of Radix Paeoniae Rubra extract on Porphyromonas gingivalis (P.g), Fusobacterium nucleatum (F.n) and Actinobacillus actinomycetes (A.a) antibacterial activity of three periodontal pathogenic bacteria, and the potential molecular mechanism and pharmacological activity of Radix Paeoniae Rubra in the prevention and treatment of chronic periodontitis (CP) were studied by network pharmacology method. Methods　 The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of three periodontal pathogens were determined by multiple dilution method. Tcmsp database was used to obtain chemical components and action targets of Radix Paeoniae Rubra, genecards and other databases were used to obtain CP disease targets, and Wayne analysis was used to take intersection. The component-target-disease of Radix Paeoniae Rubra for prevention and treatment of CP was constructed by Cytoscape 3.7.2 software. The protein protein interaction network （PPI network） was constructed by STRING database, and the enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) was carried out. Results　 The MIC of Radix Paeoniae Rubra extract against P.g, F.n, A.a were 2.0, 4.0 and 4.0 g / L respectively, and MBC were 4.0, 8.0 and 8.0 g / L, respectively. 29 active components, 2132 disease targets and 89 intersection targets were obtained. The main active components of CP treated by Radix Paeoniae Rubra are Baicalin, ellagic acid, and so on. The key targets include Interleukin-6 (IL-6) etc. By GO enrichment analysis, 126 GO items were obtained, such as protein isomerization activity etc. KEGG enrichment analysis showed that there were 110 pathways including mitogen-activated protein kinase (MAPK) signal pathway. Conclusions　 The crude extract of Radix Paeoniae Rubra has different degrees of antibacterial activity against three kinds of periodontal pathogenic bacteria. It is preliminarily expounded that the main active components, action targets and related pathways of Radix Paeoniae Rubra have important research value in the treatment of CP, which provides reference and reference for further expanding the clinical application of Radix Paeoniae Rubra.

Key words: Radix Paeoniae Rubra, chronic periodontitis, antibacterial activity, network pharmacology, action mechanism

中图分类号:

R781.4

龚怡吴泽钰王琛赵今. 赤芍的抑菌作用及其防治慢性牙周炎的机制研究[J]. 口腔医学, 2021, 41(5): 391-397.

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