载银锌介孔钙硅纳米粒子根管密封性能的研究

›› 2021, Vol. 41 ›› Issue (5): 413-417.

载银锌介孔钙硅纳米粒子根管密封性能的研究

李铭铭¹,周杨²,冷迪雅³,王莹¹,吴大明¹,李谨⁴

1. 江苏省口腔医院
2. 苏州市立医院
3. 南京医科大学附属口腔医院
4. 南京医科大学口腔医学院

收稿日期:2020-12-18 修回日期:2021-01-28 出版日期:2021-05-28 发布日期:2021-05-21
通讯作者: 吴大明 E-mail:wudamingdds@163.com
基金资助:
东南大学-南京医科大学合作研究项目

A study of the sealing properties of silver and zinc incorporated mesoporous calciumsilicate nanoparticles as a canal sealer

Received:2020-12-18 Revised:2021-01-28 Online:2021-05-28 Published:2021-05-21

摘要/Abstract

摘要： 目的评价载银锌介孔钙硅纳米粒子（silver and zinc incorporated mesoporous calcium-silicate nanoparticles, Ag-Zn-MCSNs）作为根管封闭剂的理化性能及根管密封性能。方法测定MCSNs、Ag-MCSNs、Zn-MCSNs、Ag-Zn-MCSNs（Ag∶Zn=1∶1，物质的量比）、AH-Plus糊剂的pH值、溶解度及流动性。单根恒牙根管预备后，以MCSNs、Ag-MCSNs、Zn-MCSNs、Ag-Zn-MCSNs、AH-Plus作为根管封闭剂，热牙胶技术充填根管。利用粪肠球菌（Enterococcus faecalis，E. faecalis）（ATCC 29212）渗漏法评估根管密封性能，Micro-CT扫描评估根管充填的空隙率。结果 4种纳米材料糊剂均为强碱性，溶解度小，具有良好的流动性（P＜0.05）；Ag-MCSNs组的渗漏样本最少（25%），其次是Ag-Zn-MCSNs和AH-Plus组（35%），MCSNs组最多（50%）（P＞0.05）；实验组的平均空隙率为2.09%，根尖段空隙率最高，其次是根中段和根上段（P＜0.05）。结论 MCSNs糊剂具有良好的流动性，Ag-MCSNs和Ag-Zn-MCSNs显著减少E. faecalis渗漏，根管密封性能良好。

关键词: 介孔钙硅纳米粒子, 根管封闭剂, 细菌渗漏, 根管密封性

Abstract: Abstract: Objective　To evaluate the physicochemical and sealing properties of silver and zinc incorporated mesoporous calcium-silicate nanoparticles (Ag-Zn-MCSNs) as a canal sealer. Methods The pH values, solubility and flow values of MCSNs, Ag-MCSNs, Zn-MCSNs, Ag-Zn-MCSNs(Ag:Zn=1:1, molar ratio) and AH-Plus were measured. MCSNs, Ag-MCSNs, Zn-MCSNs, Ag-Zn-MCSNs and AH-Plus were used as canal sealers and prepared permanent teeths of single-canal root were obturated using warm vertical compaction technique. Enterococcus faecails (ATCC 29212) leakage test was used to evaluate the sealing properties and Micro-CT was used to calculate the percentage of voids within canals. Results The solution of four groups was strongly alkaline. They had lower solubility and higher flow values (P <0.05). Ag-MCSNs group had least leaked samples(25%), followed by Ag-Zn-MCSNs and AH-Plus groups(35%), while MCSNs group had most leaked samples(50%)(P>0.05). All experimental groups showed low ratios of voids after obturation on average 2.09% (P＞0.05), which had the largest values in the apical sections, followed by the middle and coronal sections (P＜0.05). Conclusions　MCSNs paste possessed good fluidity and Ag-MCSNs, Ag-Zn-MCSNs could reduce E.faecalis leakage, which showed good sealing properties.

Key words: mesoporous calcium-silicate nanoparticles, root canal sealer, bacterial leakage, root canal sealing ability

中图分类号:

R783.1

李铭铭周杨冷迪雅王莹吴大明李谨. 载银锌介孔钙硅纳米粒子根管密封性能的研究[J]. 口腔医学, 2021, 41(5): 413-417.

参考文献 16

[1]	Fan W, Wu D, Ma T, Fan B. Ag-loaded mesoporous bioactive glasses against Enterococcus faecalis biofilm in root canal of human teeth [J]. Dent Mater J, 2015, 34(1): 54-60.
[2]	Fan W, Li Y, Sun Q, Ma T, Fan B. Calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- Enterococcus faecalis and mineralization properties [J]. J Nanobiotechnology, 2016, 14(1): 72.
[3]	Zhu J, Liang R, Sun C, Xie L, Wang J, Leng D, Wu D, Liu W. Effects of nanosilver and nanozinc incorporated mesoporous calcium-silicate nanoparticles on the mechanical properties of dentin [J]. PLoS One, 2017, 12(8): e0182583.
[4]	Bapat RA, Chaubal TV, Joshi CP, Bapat PR, Choudhury H, Pandey M, Gorain B, Kesharwani P. An overview of application of silver nanoparticles for biomaterials in dentistry [J]. Mater Sci Eng C Mater Biol Appl, 2018, 91: 881-98.
[5]	Wang J, Du L, Fu Y, Jiang P, Wang X. ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum [J]. BMC Oral Health, 2019, 19(1): 84.
[6]	Huang CY, Huang TH, Kao CT, Wu YH, Chen WC. Mesoporous calcium silicate nanoparticles with drug delivery and odontogenesis properties [J]. J Endod, 2017, 43(1): 69-76.
[7]	?imundi? MM, Poklepovi? PT, Utrobi?i? A, Bago I, Puljak L. Antimicrobial efficacy of commercially available endodontic bioceramic root canal sealers: A systematic review [J]. PLoS One, 2019, 14(10): e0223575.
[8]	ElReash AA, Hamama H, Eldars W, Lingwei G, Zaen El-Din AM, Xiaoli X. Antimicrobial activity and pH measurement of calcium silicate cements versus new bioactive resin composite restorative material [J]. BMC Oral Health, 2019, 19(1): 235.
[9]	Camps J, Jeanneau C, Ayachi I, Laurent P, About I. Bioactivity of a calcium silicate-based endodontic cement (Bioroot RCS): Interactions with human periodontal ligament cells in vitro [J]. J Endod, 2015, 41(9): 1469–73.
[10]	ISO-6876. Dentistry-Root canal sealing materials [S]. London, England: International Organization for Standardization, 2012.
[11]	Antunes HS, R??as IN, Alves FR, Siqueira JF Jr. Total and specific bacterial levels in the apical root canal system of teeth with post-treatment apical periodontitis [J]. J Endod, 2015, 41(7): 1037–42.
[12]	Lertmalapong P, Jantarat J, Srisatjaluk RL, Komoltri C. Bacterial leakage and marginal adaptation of various bioceramics as apical plug in open apex model [J]. J Investig Clin Dent, 2018, 10(1): e12371.
[13]	Celikten B, Uzuntas CF, Orhan AI, Orhan K, Tufenkci P, Kursun S, DemiralpK?. Evaluation of root canal sealer filling quality using a single-cone technique in oval shaped canals: An In vitro Micro-CT study [J]. Scanning, 2016, 38(2): 133-40.
[14]	Ortiz FG, Jimeno EB. Analysis of the porosity of endodontic sealers through micro-computed tomography: A systematic review [J]. J Conserv Dent, 2018, 21(3): 238-42.
[15]	Moinzadeh AT, Zerbst W, Boutsioukis C, Shemesh H, Zaslansky P. Porosity distribution in root canals filled with gutta-percha and calcium silicate cement [J]. Dent Mater, 2015, 31(9): 1100-8.
[16]	Silva EJ, Perez R, Valentim RM, Belladona FG, De-Deus G, Lima IC, Neves AA. Dissolution, dislocation and dimensional changes of endodontic sealers after a solubility challenge: a micro-CT approach [J]. Int Endod J, 2017, 50(4): 407-14.