DMAE-CB和NACP改性牙本质粘接剂的胶原纤维保护及生物相容性研究

doi:10.13591/j.cnki.kqyx.2026.10.005

摘要/Abstract

摘要：

目的评价甲基丙烯酰氧乙基-正十六烷基-二甲基氯化铵(methacryloxyethyl cetyldimethyl ammonium chloride,DMAE-CB)与无定型纳米磷酸钙(nanoparticles of amorphous calcium phosphate,NACP)改性的牙本质粘接剂用于牙体缺损粘接修复时的胶原纤维保护及生物相容性情况。方法实验室合成DMAE-CB和NACP,按两种成分在牙本质粘接剂中所占的不同质量分数分为8组(A~H组)。收集新鲜离体第三磨牙30颗,切片后随机分组,通过原位酶谱法用荧光显微镜对8组牙本质粘接剂的粘接剂-牙本质界面进行观察。制备各组牙本质粘接剂的光固化薄片,接触角测试仪测量静态水接触角。利用光固化薄片制取浸提液,CCK-8法评价各组粘接剂浸提液在不同培养时间的细胞毒性。结果原位酶谱实验中荧光条带信号的空间分布范围对比结果显示,实验组(A~G组)粘接剂对胶原酶活性的抑制能力更强。实验组粘接剂的静态水接触角较对照组(H组)降低,差异有统计学意义(P<0.05),表明其润湿性及渗透潜力增强。CCK-8实验结果表明,各组粘接剂均具有良好生物相容性,组间差异无统计学意义(P>0.05)。结论经DMAE-CB和NACP改性的牙本质粘接剂具有更好的润湿性和更强的胶原酶的抑制能力,并表现出良好的生物相容性。

关键词: 牙本质粘接剂, 胶原酶, 润湿性, 细胞毒性

Abstract:

Objective To evaluate the collagen fiber protection and biocompatibility of dental adhesive modified with methacryloxyethyl cetyldimethyl ammonium chloride(DMAE-CB) and nanoparticles of amorphous calcium phosphate(NACP) for dental defect repair. Methods DMAE-CB and NACP were synthesized in the laboratory and divided into 8 groups (Group A-H) based on the different mass fractions of the two components in the dentin adhesive. Thirty fresh extracted third molars were collected, sectioned into slices and randomly divided into groups. The adhesive-dentin interface of 8 groups was observed using in situ zymography and fluorescence microscopy. Various light-cured dentin adhesive films were prepared, and the static water contact angle was measured using a contact angle goniometer. Extraction solutions were prepared from light-cured films, and the cytotoxicity of the 8 dentin adhesive extraction solutions was evaluated using the CCK-8 assay at different culture time points. Results The distribution range of the fluorescent signal indicated that the experimental group (Group A-G) exhibited stronger inhibition of collagenase activity compared with the control group (Group H). The static water contact angle of the experimental group was significantly lower than that of the control group(P<0.05), indicating improved wettability and penetration potential. The CCK-8 assay showed that all adhesive groups exhibited good biocompatibility, with no statistically significant difference among groups(P>0.05). Conclusion The dentin adhesive modified with DMAE-CB and NACP has improved wettability, enhanced collagenase inhibition, and good biocompatibility.

Key words: dentin adhesive, collagenase, wettability, cytotoxicity

中图分类号:

R783.3

周雨凡, 蔡荣政, 郭心睿, 张春雷, 孙宇. DMAE-CB和NACP改性牙本质粘接剂的胶原纤维保护及生物相容性研究[J]. 口腔医学, 2026, 46(10): 751-756.

ZHOU Yufan, CAI Rongzheng, GUO Xinrui, ZHANG Chunlei, SUN Yu. Study on collagen fiber protection and biocompatibility of DMAE-CB and NACP modified dentin adhesive[J]. Stomatology, 2026, 46(10): 751-756.

图/表 3

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