bFGF-胶原蛋白缓释系统促进兔下颌骨缺损修复的研究

›› 2014, Vol. 34 ›› Issue (10): 737-742.

bFGF-胶原蛋白缓释系统促进兔下颌骨缺损修复的研究

范伟伟¹,麻健丰²,朱莉³,黄志峰⁴,⁵,朱雁林⁴,⁵,周稚辉²,张金桂⁴,⁵,王燕⁴,⁵,王彦亮²

1. 温州医科大学附属口腔医院
2. 温州医学院附属口腔医院
3. 温州医学院口腔医学院
4. 温州医科大学药学院
5. 温州医科大学药学院

收稿日期:2014-02-11 修回日期:2014-03-24 出版日期:2014-10-28 发布日期:2014-10-15
通讯作者: 范伟伟 E-mail:ffaannwweeii@163.com
基金资助:
2013年浙江省大学生科技创新活动计划（新苗人才计划）资助项目;2012年浙江省医药卫生一般研究计划项目

The study of bone formation of bFGFcollagen slow release system in mandibular defects of rabbit

Received:2014-02-11 Revised:2014-03-24 Online:2014-10-28 Published:2014-10-15
Contact: Wei-wei Fan E-mail:ffaannwweeii@163.com

摘要/Abstract

摘要： 目的观察评估bFGF-胶原蛋白缓释系统作为骨修复材料的可能性。方法 60只日本大耳白兔随机分成3组：bFGF胶原海绵组，胶原海绵组及空白对照组。手术建立兔双侧下颌骨洞穿性骨缺损模型并植入相应生物材料。分别在2、6、8、12周对造模部位取材，行大体观察、CT影像学检测和组织学观察。结果大体观察和CT影像学检测显示实验组骨创愈合速度较快。组织学观察可见实验组成骨情况优于同期对照组，胶原蛋白在体内作用时间持续至6周以前，其持续释放的bFGF颗粒对骨缺损修复有明显的促进作用，但发挥作用时间主要在12周前。结论 bFGF-胶原蛋白缓释系统对兔下颌骨缺损具有良好的修复效果，其发挥作用时间主要在骨缺损修复早期。此外，胶原蛋白海绵本身对骨缺损修复早期亦具有一定促进作用。

关键词: bFGF, 胶原蛋白海绵, 缓释, 临界性骨缺损

Abstract: Objective To study the possibility of using bFGFcollagen slow release system for repairing bone defect.Methods 60 Japanese white rabbits were randomly divided into three groups: the bFGF collagen sponge group,the collagen sponge group and the control group.Then the bone defects were made in the rabbits′ bilateral mandibles,and the biological materials corresponding with each group were inserted into defects.15 of animals were sacrificed at 2、6、8、12 weeks to be evaluated with anatomy,CT studies and histology.Results General observation and CT imaging showed a faster velocity of bone formation in the control groups.Histological observation showed that the effect of bone repairing in the experimental group was much better than that in the control group.The collagen in vivo lasted until 6 weeks ago,and the bFGF which was continuously released from it had obvious promoting effect on bone defect repair,while the action time was mainly 12 weeks ago.Conclusions The bFGFcollagen slow release system has great promoting effect on repairing rabbit mandibular defect,while the action time is mainly in the early stage of bone defect repairing.Besides,the collagen sponge itself also has promoting effect on mandibular defect repair.

Key words: basic fibroblast growth factor, collagen sponge, slow-release, critical size defect

中图分类号:

R329.29

范伟伟麻健丰朱莉黄志峰朱雁林周稚辉张金桂王燕王彦亮. bFGF-胶原蛋白缓释系统促进兔下颌骨缺损修复的研究[J]. 口腔医学, 2014, 34(10): 737-742.

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